Production of Fumonisin B Analogues and Related Compounds by <i>Fusarium globosum</i>, a Newly Described Species from Corn

Sydenham, Eric W.; Shephard, Gordon S.; Stockenström, Sonja; Rheeder, John P.; Marasas, and Walter F. O.; Merwe, Margaretha J. van der

doi:10.1021/jf9607066

Cited by 14 publications

(7 citation statements)

References 40 publications

(52 reference statements)

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“…Previous studies on isolates from Europe and the U.S. have noted a similar pattern of toxin production (Moretti et al, 1996). F. globosum is a recently described species, and its profile of toxin production has previously been restricted to a report of fumonisin but not MON production (Sydenham et al, 1997). This study confirms these previous results as to its production of fumonisins at relatively low levels in comparison to that of F. verticillioides and F. proliferatum and shows further for the first time that F. globosum is also a weak producer of FUS and BEA.…”

Section: Resultssupporting

confidence: 88%

“…Within this classification, F. proliferatum corresponds to the D mating population and F. subglutinans isolated predominantly from corn corresponds to the E mating (Rheeder et al, 1996). Of 17 strains investigated, all produced fumonisins but none produced MON when grown in culture on corn kernels (Sydenham et al, 1997). Fusaproliferin (FUS) is a recently identified sesterterpene purified from corn cultures of F. proliferatum ITEM-1494 (isolated from corn ear rot in northern Italy) using toxicity to brine shrimp larvae (Artemia salina L.) as a guide to extract fractionation (Ritieni et al, 1995;Santini et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

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Production of the Mycotoxins Fusaproliferin and Beauvericin by South African Isolates in the Fusarium Section Liseola

Shephard

Sewram

Nieuwoudt

et al. 1999

J. Agric. Food Chem.

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The production of fusaproliferin (FUS), a recently described mycotoxin, and beauvericin (BEA), a mycotoxin recently reported to co-occur with FUS in Fusarium-infected corn, by South African isolates in the Fusarium section Liseola, was investigated. Five isolates each of F. verticillioides, F. proliferatum, F. subglutinans, and F. globosum were cultured on corn kernels. Four each of the five South African isolates of F. proliferatum and F. subglutinans produced FUS (10-1725 and 330-2630 mg/kg, respectively). BEA was produced by four of the F. proliferatum strains (310-1130 mg/kg) and three of the F. subglutinans strains (140-700 mg/kg). The isolates of F. verticillioides failed to produce significant levels of either of these secondary metabolites. F. globosum was a weak producer of both in that one isolate of five produced 25 mg/kg FUS and five out of five produced BEA at levels ranging between 10 and 110 mg/kg. To further characterize these strains, their production of fumonisins B(1), B(2), and B(3), as well as moniliformin, was investigated. Of the four species investigated, fumonisins were produced by all except F. subglutinans, which in turn was the only species whose isolates in this study produced moniliformin (four of five isolates, ranging from 155 to 2095 mg/kg). Analysis of visibly Fusarium-infected home-grown corn collected in the Transkei region of the Eastern Cape Province of South Africa showed that nine of the ten samples contained low levels of FUS (up to 62 microg/kg), whereas all ten samples showed BEA contamination ranging from 8 to 1734 microg/kg with a mean of 258 microg/kg.

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Section: Resultssupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Production of the Mycotoxins Fusaproliferin and Beauvericin by South African Isolates in the Fusarium Section Liseola

Shephard

Sewram

Nieuwoudt

et al. 1999

J. Agric. Food Chem.

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“…globosum is morphologically and phylogenetically related to other species within the G. fujikuroi complex (41,45). This affiliation is supported by its production of fumonisin mycotoxins (48). Interestingly, all tested strains of F. globosum were very sensitive to BOA even though they were isolated from maize and wheat, which are producers of MBOA and BOA.…”

Section: Discussionmentioning

confidence: 87%

Detoxification of Corn Antimicrobial Compounds as the Basis for Isolating Fusarium verticillioides and Some Other Fusarium Species from Corn

Glenn

Hinton

Yates

et al. 2001

Appl Environ Microbiol

115

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The preformed antimicrobial compounds produced by maize, 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3-one and its desmethoxy derivative 2,4-dihydroxy-2H-1,4-benzoxazin-3-one, are highly reactive benzoxazinoids that quickly degrade to the antimicrobials 6-methoxy-2-benzoxazolinone (MBOA) and 2-benzoxazolinone (BOA), respectively. Fusarium verticillioides ‫؍(‬ F. moniliforme) is highly tolerant to MBOA and BOA and can actively transform these compounds to nontoxic metabolites. Eleven of 29 Fusarium species had some level of tolerance to MBOA and BOA; the most tolerant, in decreasing order, were F. verticillioides, F. subglutinans, F. cerealis ‫؍(‬ F. crookwellense), and F. graminearum. The difference in tolerance among species was due to their ability to detoxify the antimicrobials. The limited number of species having tolerance suggested the potential utility of these compounds as biologically active agents for inclusion within a semiselective isolation medium. By replacing the pentachloronitrobenzene in Nash-Snyder medium with 1.0 mg of BOA per ml, we developed a medium that resulted in superior frequencies of isolation of F. verticillioides from corn while effectively suppressing competing fungi. Since the BOA medium provided consistent, quantitative results with reduced in vitro and taxonomic efforts, it should prove useful for surveys of F. verticillioides infection in field samples.

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“…, 1996). Of these, F. globosum isolates were subsequently shown to produce fumonisins (Sydenham et al. , 1997; Moses et al.…”

Section: Introductionmentioning

confidence: 99%

Quantitative detection ofFusariumpathogens and their mycotoxins in South African maize

et al. 2011

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The distribution and co-occurrence of four Fusarium species and their mycotoxins were investigated in maize samples from two susceptible cultivars collected at 14 localities in South Africa during 2008 and 2009. Real-time PCR was used to quantify the respective Fusarium species in maize grain, and mycotoxins were quantified by multi-toxin analysis using HPLC-MS. In 2008, F. graminearum was the predominant species associated with maize ear rot in the eastern Free State, Mpumalanga and KwaZulu-Natal provinces, while F. verticillioides was predominant in the Northwest, the western Free State and the Northern Cape provinces. In 2009, maize ear rot infection was higher and F. graminearum became the predominant species found in the Northwest province. Fusarium subglutinans was associated with maize ear rot in both years at most of the localities, while F. proliferatum was not detected from any of the localities. Type B trichothecenes, especially deoxynivalenol, and zearalenone were well correlated with the amount of F. graminearum, fumonisins with F. verticillioides, and moniliformin and beauvericin with F. subglutinans. This information is of great importance to aid understanding of the distribution and epidemiology of Fusarium species in South Africa, and for predicting mycotoxin contamination risks and implementing preventative disease management strategies.

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Production of Fumonisin B Analogues and Related Compounds by Fusarium globosum, a Newly Described Species from Corn

Cited by 14 publications

References 40 publications

Production of the Mycotoxins Fusaproliferin and Beauvericin by South African Isolates in the Fusarium Section Liseola

Production of the Mycotoxins Fusaproliferin and Beauvericin by South African Isolates in the Fusarium Section Liseola

Detoxification of Corn Antimicrobial Compounds as the Basis for Isolating Fusarium verticillioides and Some Other Fusarium Species from Corn

Quantitative detection ofFusariumpathogens and their mycotoxins in South African maize

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