Production of fumaric acid by immobilized Rhizopus arrhizus RH 7-13-9# on loofah fiber in a stirred-tank reactor

Liu, Huan; Zhao, Shijie; Jin, Yuhan; Yue, Xuemin; Deng, Li; Wang, Fang; Tan, Tianwei

doi:10.1016/j.biortech.2017.07.185

Cited by 28 publications

(14 citation statements)

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“…Stirred tanks are widely used in research and industry due to their ease of control and handling during operation and the fact that they have low operation costs at the industrial scale, providing the process with great capacity and reliability [70].…”

Section: Stirred Tanksmentioning

confidence: 99%

“…The simplest use of immobilized biomass is suspending the particles in a stirrer-tank reactor (slurry reactor). In this process, many natural solids used as supports where the immobilization of microorganisms has been studied, for example, eggshell [75] or loofah fiber [70]. Some synthetic supports have been tried as well: plastic or metal nets [76].…”

Section: Stirred Tank With Immobilized Biomassmentioning

confidence: 99%

“…Some synthetic supports have been tried as well: plastic or metal nets [76]. Using loofah fiber as a support, promising yields (0.21 g of fumaric acid per gram of glucose) and concentrations (30.3 g·L −1 in the broth) were obtained, proving the potential use of loofah fiber to immobilize biomass in the fermentative fumaric acid production process [70].…”

Section: Stirred Tank With Immobilized Biomassmentioning

confidence: 99%

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Fumaric Acid Production: A Biorefinery Perspective

et al. 2018

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The increasing scarcity of fossil raw materials, together with the need to develop new processes and technology based on renewable sources, and the need to dispose of an increasing amount of biomass-derived waste, have boosted the concept of biorefineries. Both 1G and 2G biorefineries are focused on the obtention of biofuels, chemicals, materials, food and feed from biomass, a renewable resource. Fumaric acid, and most compounds involved in the Kreb cycle, are considered key platform chemicals, not only for being acidulants and additives in the food industry, but also for their prospective use as monomers. This review is focused on the biotechnological processes based on fungi, mainly of the Rhizopus genus, whose main product is fumaric acid, on the process conditions, the bioreactors and modes of operation and on the purification of the acid once it is produced.

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Section: Stirred Tanksmentioning

confidence: 99%

Section: Stirred Tank With Immobilized Biomassmentioning

confidence: 99%

Section: Stirred Tank With Immobilized Biomassmentioning

confidence: 99%

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Fumaric Acid Production: A Biorefinery Perspective

et al. 2018

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“…Mycelia immobilized fermentation was utilized for fumaric acid production in the one-step fermentation. Loofah fiber (prepared from dried Luffa cylindrica and purchased at Jinhua in the Zhejiang Province, China) was shown to be an available carrier for R. arrhizus RH-7-13-9# in the authors' previous work (Liu et al 2017). Small loofah fiber cubes of 0.5 to 1 cm 3 were prepared, and 6.5 g/L loofah fiber cubes were added into a 5 L stirred-tank reactor as mycelia immobilized carriers.…”

Section: One-step Fermentation Processmentioning

confidence: 99%

“…In addition to the nitrogen source, the agitation speed of the stirred-tank reactor is important in fumaric acid production. A higher agitation speed destroys the growth of the cells due to the shear force, while a lower agitation speed influences the oxygen transfer and inhibits the accumulation of fumaric acid (Fu et al 2010;Liu et al 2017). Therefore, different agitation speeds are applied in the seed culture phase (200 rpm) and fumaric acid production phase (400 rpm) during two-step fermentation (Singh et al 2021;Wu et al 2018).…”

Section: Two-stage Regulation Of Agitation Speed In the Stirred-tank Reactormentioning

confidence: 99%

Applications of one-Step environmentally-friendly fermentation method to produce fumaric acid with immobilized Rhizopus arrhizus in stirred-tank reactor

Ning

Yang

Zhao

et al. 2021

BioRes

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The traditional two-step cultivation mode for fungal cultivation is commonly divided into the seed stage (usually 24 to 48 h) and the production stage (usually 96 to 144 h). The use of two stages prolongs the total production cycle and generates excess wastewater. In this work, an efficient and environmentally friendly one-step fermentation method was applied for the production of fumaric acid by immobilized Rhizopus arrhizus in a stirred-tank reactor. The nitrogen source content and the agitation speed of the reactor were optimized as the two critical factors in the one-step fermentation process; the fumaric acid production of 51 g/L with a yield of 0.42 g/g glucose was comparable with the product from the traditional two-step fermentation process. Furthermore, the total production cycle was shortened to 96 h, and the amount of wastewater was reduced due to the avoidance of the seed culture process. Thus, utilization of the one-step fermentation method to produce fumaric acid was shown to be preferable feasibility and environmental-friendliness. This is a promising method for industrial production of fumaric acid and other high-value biochemicals by fungus.

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