Production of fuel ethanol from softwood by simultaneous saccharification and fermentation at high dry matter content

Hoyer, Kerstin; Galbe, Mats; Zacchi, Guido

doi:10.1002/jctb.2082

Cited by 81 publications

(67 citation statements)

References 36 publications

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“…These findings were in agreement with Romaní et al (2014) and Hoyer et al (2009), who reported better performance for SSF than PSSF. The PSSF process significantly increased the % cellulose conversion only when the % cellulose conversion and ethanol concentration were low in the SSF process, but otherwise, it did not have a significant effect, or even had a negative effect when a high cellulose conversion and ethanol content were obtained without a pre-hydrolysis step (Hoyer et al 2013).…”

Section: Pssf Vs Ssfsupporting

confidence: 92%

“…Moreover, according to Hoyer et al (2009) andLópez-Linares et al (2014a), the use of higher pretreated biomass loadings (up to 10%) resulted in a higher ethanol concentration and, at same time, no significant decrease in the cellulose conversion occurred. These results confirmed that there was no end-product accumulation during the SSF process when using a less than 10% biomass concentration, which was the limit to obtain good cellulose conversion (Olofsson et al 2008).…”

Section: Simultaneous Saccharification and Fermentation (Ssf)mentioning

confidence: 98%

“…Environmentally friendly energy resources for alternative fuels have increased in the last decade, with the aim of progressively replacing fossil fuels and reducing the amount of greenhouse gases released into the atmosphere (Hoyer et al 2009;Menegol et al 2016;Ventorino et al 2017). Bioethanol, produced from lignocellulosic biomass, is a possible alternative to fossil fuels used in transportation (Saini et al 2015).…”

Section: Introductionmentioning

confidence: 99%

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Effect of Cellulase, Substrate Concentrations, and Configuration Processes on Cellulosic Ethanol Production from Pretreated Arundo donax

Aliberti¹,

Ventorino²,

Robertiello³

et al. 2017

BioResources

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Arundo donax was used to investigate the effect of the enzyme and substrate concentrations on hydrolysis, pre-hydrolysis and simultaneous saccharification and fermentation (PSSF), in comparison to simultaneous saccharification and fermentation (SSF). Hydrolysis was performed at 37 and 50 °C. At the highest biomass (10%) and enzyme (69.6 FPU/g cellulose) loadings, the highest glucose concentration (32.4 g/L) was obtained (at 50 °C). SSF resulted in a cellulose conversion (91.9%) and an ethanol concentration (19.8 g/L) higher than what was obtained using PSSF at 37 °C (86.9% and 18.8 g/L, respectively) and PSSF at 50 °C (81.6% and 17.7 g/L, respectively). A positive correlation between the cellulase concentration, cellulose conversion, and ethanol content was observed. In PSSF, the increase in the solids loadings caused a reduction in the % cellulose conversion, but the ethanol concentration in PSSF and SSF increased. SSF appeared to be the most advantageous process for bioethanol production from A. donax.

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Section: Pssf Vs Ssfsupporting

confidence: 92%

Section: Simultaneous Saccharification and Fermentation (Ssf)mentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Effect of Cellulase, Substrate Concentrations, and Configuration Processes on Cellulosic Ethanol Production from Pretreated Arundo donax

Aliberti¹,

Ventorino²,

Robertiello³

et al. 2017

BioResources

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“…24,25 Very recently, interesting results were discussed on SSF in a fluidized bed reactor (MSFBR) using glucose. Molasses gave comparative ethanol productivity in comparison to glucose in the MSFBR.…”

Section: Introductionmentioning

confidence: 99%

Diluted Acid Pretreatment of Pinus Radiata for Bioethanol Production Using Immobilized Saccharomyces Cerevisiae Ir2-9 in a Simultaneous Saccharification and Fermentation Process

Franco¹,

Mendonça²,

Marcato

et al. 2011

J. Chil. Chem. Soc.

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The production of bioethanol from pretreated lignocellulosic materials requires the utilization of microorganisms adapted to ferment the materials in conditions were high consistency, temperatures and inhibitors concentrations were commonly found. The yeast immobilization in calcium alginate capsules has been reported to enhance the yeast protection and increase the efficiency in the fermentation process. In this work, it was investigated the use Saccharomyces cerevisiae immobilized in calcium alginate and its performance in simultaneous saccharification and fermentation (SSF) of diluted-acid-pretreated Pinus radiata. Results showed that when immobilized yeast was used, the bioethanol yield from pretreated wood was higher than with free yeast cells during a SSF process. Maximum ethanol yield obtained from the acid pretreated and milled wood chips was 153 L/ton wood, while from the hydrolysate fraction it was 18 L/ton wood. The sum of ethanol produced from dilute acid pretreated P. radiata for both solid and liquid fractions was 171 L ethanol/ton wood from a maximum theoretical of 236 L/ ton pretretated wood (or 72% of conversion).

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“…Manzanares et al [29] also found that with increasing initial DM content, a separate saccharification step improves fermentation of liquid hot waterpretreated olive-pruning biomass. Finally, Hoyer et al [30] reported that with increasing DM content, even 4 h of saccharification could significantly improve fermentation of softwoods.…”

Section: Resultsmentioning

confidence: 99%

Utilization Of Household Food Waste For The Production Of Ethanol At High Dry Material Content

Μάτσακας¹,

Kekos²,

Loizidou³

et al. 2015

Solid Waste as a Renewable Resource

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Background: Environmental issues and shortage of fossil fuels have turned the public interest to the utilization of renewable, environmentally friendly fuels, such as ethanol. In order to minimize the competition between fuels and food production, researchers are focusing their efforts to the utilization of wastes and by-products as raw materials for the production of ethanol. household food wastes are being produced in great quantities in European Union and their handling can be a challenge. Moreover, their disposal can cause severe environmental issues (for example emission of greenhouse gasses). On the other hand, they contain significant amounts of sugars (both soluble and insoluble) and they can be used as raw material for the production of ethanol. Results: Household food wastes were utilized as raw material for the production of ethanol at high dry material consistencies. A distinct liquefaction/saccharification step has been included to the process, which rapidly reduced the viscosity of the high solid content substrate, resulting in better mixing of the fermenting microorganism. This step had a positive effect in both ethanol production and productivity, leading to a significant increase in both values, which was up to 40.81% and 4.46 fold, respectively. Remaining solids (residue) after fermentation at 45% w/v dry material (which contained also the unhydrolyzed fraction of cellulose), were subjected to a hydrothermal pretreatment in order to be utilized as raw material for a subsequent ethanol fermentation. This led to an increase of 13.16% in the ethanol production levels achieving a final ethanol yield of 107.58 g/kg dry material. Conclusions: In conclusion, the ability of utilizing household food waste for the production of ethanol at elevated dry material content has been demonstrated. A separate liquefaction/saccharification process can increase both ethanol production and productivity. Finally, subsequent fermentation of the remaining solids could lead to an increase of the overall ethanol production yield.

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Production of fuel ethanol from softwood by simultaneous saccharification and fermentation at high dry matter content

Cited by 81 publications

References 36 publications

Effect of Cellulase, Substrate Concentrations, and Configuration Processes on Cellulosic Ethanol Production from Pretreated Arundo donax

Effect of Cellulase, Substrate Concentrations, and Configuration Processes on Cellulosic Ethanol Production from Pretreated Arundo donax

Diluted Acid Pretreatment of Pinus Radiata for Bioethanol Production Using Immobilized Saccharomyces Cerevisiae Ir2-9 in a Simultaneous Saccharification and Fermentation Process

Utilization Of Household Food Waste For The Production Of Ethanol At High Dry Material Content

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