Production of fructooligosaccharides by β‐fructofuranosidase from Aspergillus sp 27H

Abstract

β-fructofuranosidase (EC 3.2.1.26) from Aspergillus sp 27H isolated from soil was investigated for production of fructooligosaccharides (FOS) using whole cells. It possesses hydrolytic and transfructosylating activities that can be altered by modifying the reaction conditions. The optimal conditions for the transfructosylating activity occur in the pH range 5.5-6.0 and at 60• C, while hydrolytic activity was highest at pH 4.0 and 55• C. At low sucrose concentration (10 g dm −3 ) there was rapid conversion of sucrose to glucose and fructose and very low concentrations of FOS were obtained. However, at sucrose concentrations higher than 216 g dm −3 the concentrations of hydrolysis products were reduced. Under the following conditions: pH 5.5, temperature 40• C, sucrose concentration 615 g dmand enzyme concentration 20β-fructofuranosidase units g −1 of sucrose, the FOS concentration reached a maximum value of 376 g dm −3 (234 g dm −3 1-kestose and 142 g dm −3 nystose) and the proportion of FOS in the solids in the reaction mixture was 600-620 g kg −1 at 6 h. These results suggest that β-fructofuranosidase from Aspergillus sp 27H could be an appropriate enzyme for the commercial production of FOS.

Efficient Fructooligosaccharide Synthesis with a Fructosyltransferase fromAspergillus aculeatus

“…The rest of the carbohydrates in the mixture were fructose (marginal), glucose, and the remaining sucrose. These values compared well with those reported using the crude Pectinex Ultra SP -L under similar experimental conditions [32,56] and with fructosyltransferases from other Aspergillus strains [8,10,64,65] . …”

Beet Sugar Syrup and Molasses as Low-Cost Feedstock for the Enzymatic Production of Fructo-oligosaccharides

“…As syrup and molasses are coloured materials and the FOS specifications for human 25 nutrition are very restrictive in this subject, the FOS obtained by the methods described in We are very grateful to Azucarera Ebro (Valladolid, Spain) for supplying beet sugar syrup 10 and molasses. We thank Drs.…”

Separation of fructooligosaccharides using zeolite fixed bed columns

“…They are also known as neo-sugars and have numerous beneficial, favorable functional properties, including improvement of the intestinal microflora [2], non-cariogenicity, being diabetic-friendly products, there are clinical studies showing that in some situations they can decrease the serum levels of cholesterol, phospholipids and triglycerides [3,4] and of relieving constipation [5]. FOS have been widely used in bio-industries as sweeteners because of their favorable functional properties [6].…”