Production of fructooligosaccharide syrup from sucrose in combination with palatinose and trehalose

Markosyan, A. A.; Abelyan, L. A.; Adamyan, M. O.; Ekazhev, Z. D.; Akopyan, Zh. I.; Abelyan, V. A.

doi:10.1134/s0003683807040047

Cited by 8 publications

(5 citation statements)

References 16 publications

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“…In previous report, maltooligosylsucrose with 81.7% yield was synthesized by this amylomaltase using raw tapioca starch as a glucosyl donor and sucrose as acceptor [34]. For synthesis of other functional oligosaccharides, IMOs produced by maltogenic amylase in combination with ␣-glucanotransferase gave 68.0% product yield [55] and fructooligosaccharides synthesis catalyzed by fructofuranosidase gave 49.8% yield [56]. Hence, the PGs yield obtained in this study is quite good.…”

Section: Optimization Of the Synthesis Of Glucoside Productssupporting

confidence: 53%

Acceptor specificity of amylomaltase from Corynebacterium glutamicum and transglucosylation reaction to synthesize palatinose glucosides

Naumthong

Pongsawasdi

2015

Process Biochemistry

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Section: Optimization Of the Synthesis Of Glucoside Productssupporting

confidence: 53%

Acceptor specificity of amylomaltase from Corynebacterium glutamicum and transglucosylation reaction to synthesize palatinose glucosides

Naumthong

Pongsawasdi

2015

Process Biochemistry

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“…pullulans, etc.) capable of producing intracellular FTase, purified FTase from culture media, immobilized whole cells, and immobilized FTase in supporting materials such as calcium alginate and ligonocellulosic materials. ,, Among them, immobilization technology was extensively employed to enhance enzyme stability and successive recycling capacity. However, from the view of industrial applications, the high immobilized carrier cost and multistep operations are limited factors.…”

Section: Discussionmentioning

confidence: 99%

Highly Efficient Fructooligosaccharides Production by an Erythritol-Producing Yeast Yarrowia lipolytica Displaying Fructosyltransferase

Zhang¹,

An²,

Li³

et al. 2016

J. Agric. Food Chem.

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Currently, fructooligosaccharides (FOS) are industrially transformed from sucrose by purified enzymes or fungi cells. However, these methods are expensive and time-consuming. An economical approach to producing FOS using erythritol-producing yeast cells was described in this study. Fructosyltransferase from Aspergillus oryzae was displayed on the cell surface of Yarrowia lipolytica, resulting in an engineered strain capable of transforming sucrose to FOS. An amount of 480 g/L FOS was produced within 3 h in a solution of 800 g/L sucrose and 5 g/L cells (dry cell weight, DCW) at pH 6.0 and 60 °C, with a yield of 60% of total sucrose and a productivity of 160 g/(L·h). The yeast pastes from the erythritol industry can be repeatedly used as the whole-cell catalysts at least 10 times by this newly developed approach. This efficient method is attractive for the large-scale production of FOS from sucrose.

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“…Considering literature data, this enzyme has the same optimum temperature of some Aspergillus strains, but the optimum pH value is considerably lower (Ghazi et al. 2007; Markosyan et al. 2007).…”

Section: Discussionmentioning

confidence: 78%

“…Under these conditions, the FTA was more than 10 times higher than the hydrolytic activity. Considering literature data, this enzyme has the same optimum temperature of some Aspergillus strains, but the optimum pH value is considerably lower (Ghazi et al 2007;Markosyan et al 2007). Chen and Liu (1996) studied the b-fructofuranosidase from Aspergillus japonicus, an enzyme showing both hydrolytic and transfructosilating activities, optimum activity being achieved at pH 5.0-6.0 and temperatures between 55 and 65C for fructose transfer, and at pH 4.0 and 65C for the hydrolytic activity.…”

mentioning

confidence: 79%

SYNTHESIS OF FRUCTOOLIGOSACCHARIDES USING EXTRACELLULAR ENZYMES FROMRHODOTORULASP

Hernalsteens

Maugeri

2010

Journal of Food Biochemistry

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Considering the great biodiversity of the still unexploited Brazilian environment, efforts were made to select microorganisms capable of producing fructooligosaccharide (FOS). Amongst 495 interesting isolated yeast strains, one in particular, identified as a Rhodotorula red yeast strain isolated from flowers from the Brazilian tropical biomass, was able to produce an effective thermostable extracellular enzyme, with both sucrose hydrolytic and transfructosylating activities. The best operational conditions for this enzyme were determined to be in a pH range from 3.8 to 4.3 and at temperatures of around 65C. Under these conditions, the transfructosylating activity reached its maximum, being more than 10 times higher than the hydrolytic activity. The synthesis of fructooligosaccharides from sucrose resulted in total conversion of the substrate into FOS (48%), glucose (31.3%) and fructose (20.7%). In addition, it was shown that the synthesized FOS had a prebiotic effect, promoting the growth of probiotic bacteria. PRACTICAL APPLICATIONS The search for new enzymes for oligosaccharide production, using either microbial screening or molecular engineering, became necessary as a result of the increasing number of applications of these carbohydrates in the cosmetic, agrochemical, pharmaceutical and food industries. In this work, the Rhodotorula sp. isolated from Brazilian biomes was shown to be capable of producing an extracellular fructosyltransferase enzyme with good prospects for further studies aiming an industrial application, because it can convert sucrose into fructooligosaccharide (FOS), giving high yields (50%). It is worthwhile to emphasize the high optimum temperature and low pH value of the enzyme operation, which make it possible to work in a nonsterile environment for FOS production. It is also remarkable the ability of this yeast type (Rhodotorula), of growing on diverse alternative media and agricultural by‐products (molasses, corn steel liquor, grape must, whey ultrafiltrate).

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Production of fructooligosaccharide syrup from sucrose in combination with palatinose and trehalose

Cited by 8 publications

References 16 publications

Acceptor specificity of amylomaltase from Corynebacterium glutamicum and transglucosylation reaction to synthesize palatinose glucosides

Acceptor specificity of amylomaltase from Corynebacterium glutamicum and transglucosylation reaction to synthesize palatinose glucosides

Highly Efficient Fructooligosaccharides Production by an Erythritol-Producing Yeast Yarrowia lipolytica Displaying Fructosyltransferase

SYNTHESIS OF FRUCTOOLIGOSACCHARIDES USING EXTRACELLULAR ENZYMES FROMRHODOTORULASP

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