Production of filmable medium-chain-length polyhydroxyalkanoates produced from glycerol by Pseudomonas mediterranea

Impact of different by-products from the biodiesel industry and bacterial strains on the production, composition, and properties of novel polyhydroxyalkanoates containing achiral building blocks

“…Similarly, Kawata and Aiba (2010) obtained 0.13 g PHA per gram of CG in an orbital shaker at 30 • C/60 h/150 rpm using Halomonas sp. Some strains have been screened for the production of PHAs in culture with CG: Haloferax mediterranei (Hermann- Krauss et al, 2013), Pseudomonas mediterranea (Pappalardo et al, 2014), Bacillus megaterium (Naranjo et al, 2013), and Bacillus sphaericus NII 0838 (Sindhu et al, 2011).…”

“…The detection of these building blocks, especially the first two building blocks, is a genuine scientific novelty given that they are absolutely novel PHA constituents. Pappalardo et al (2014) also determined the composition of polyesters obtained from CG by P. mediterranea, and used GC-MS and nuclear magnetic resonance (NMR) analysis to verify that the PHAs were composed of six blocks for the construction of long and medium chains: 3-hydroxyhexanoate (C6), 3-hydroxyoctanoate (C8), 3-hydroxydecanoate (C10), 3-hydroxydodecanoate (C12), cis-3-hydroxydodec-5-enoate (C12: 5 ), and cis-3-hydroxydodec-6-enoate (C12:1 6 ). Campos et al (2014) optimized the production of biopolyesters from C. necator IPT 026 with CG II and primarily identified building blocks of short chain of PHAs, such as 3-hydroxybutyrate (23.80-56.05 mol%) and 3-hydroxypentanoate (7.85 mol%), and blocks of medium chain, such as 8-hydroxynonanoate (27.82-55.74 mol%).…”

Bioconversion of Styrene to Poly(hydroxyalkanoate) (PHA) by the New Bacterial Strain <i>Pseudomonas putida</i> NBUS12

“…In spite of the wide application potentials of PHAs, most, particularly medium-chain length PHA (mcl- PHA), have yet to be commercialized due to their high production costs. One effective way to reduce production costs is the use of less expensive carbon substrates (3, 4, 23, 24, 31). The pyrolysis of styrene-based plastic waste, particularly PS, can produce pyrolytic oil that comprises up to 75% styrene (22).…”

Bioconversion technologies of crude glycerol to value added industrial products

“…mcl-PHA produced through microbial fermentation by Pseudomonas spp. P. mediterranea 9.1 (CFBP 5447) using crude glycerol as a carbon source is a potential to use as a softener for biopolymeric blends of food packaging and medical devices material [46]. Bacillus thuringiensis EGU45 also reported for its efficiency in production of different PHA’s under optimized nitrogen conditions using crude glycerol as a carbon source.…”