Production of fibronectin by HUH6 Cl5 cell line established from a human hepatoblastoma

Tanaka, Masanobu; Kawamura, Kazuo; Fang, Meiqi; Higashino, Kazuya; Kishimoto, Seiji; Nakabayashi, Hidekazu; Sato, Jiro

doi:10.1016/0006-291x(83)91037-9

Cited by 10 publications

(5 citation statements)

References 11 publications

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“…Clonal derivative of HepG2-strong contact inhibition, high albumin production and high ␣-fetoprotein (Wang et al, 1998) HuH-7 Frequently used in viral infection studies-initially grown in fully defined synthetic medium (Nakabayashi et al, 1982) HuH-6 C15 Possesses Fibronectin producing enzyme, mainly used in a limited number of viral infection studies (Hepatitis B) (Tanaka et al, 1983) HLE/HLF Isolated from a HCC, HLE are epithel like cells and produces ␣-fetoprotein-HLF cells resemble fibroblasts and do not produce ␣-fetoprotein (Dor et al, 1975) NPLC A non parenchymal liver cell line, rarely used (Carlin et al, 1988) Tong Established from a HCC of an HBsAg-positive Italian male-secretes ␣-fetoprotein, albumin and ␣-1-antitrypsin (Stevenson et al, 1987) A number of other cell lines, which have been isolated from hepatic human tissue are listed in Table 2. They have been used occasionally in acute toxicity experiments, but not in genotoxicity studies.…”

Section: Transfectants Of Hepg2 Cells and New Hepatoma Lines Which Armentioning

confidence: 99%

Use of human-derived liver cell lines for the detection of environmental and dietary genotoxicants; current state of knowledge

et al. 2004

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Section: Transfectants Of Hepg2 Cells and New Hepatoma Lines Which Armentioning

confidence: 99%

Use of human-derived liver cell lines for the detection of environmental and dietary genotoxicants; current state of knowledge

et al. 2004

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“…The human HB cell lines HuH6 and HepT1 were used for all experiments [5] – [7] . HepT1was derived from a multifocal embryonal HB.…”

Section: Methodsmentioning

confidence: 99%

“…Experimental anti-tumor therapies are frequently tested for efficacy in murine subcutaneous tumor models. Established HB cell lines that are currently used include the mixed HB cell line HuH6 and the embryonal HB cell line HepT1 [5] – [7] . For example studies of blockade of VEGF [8] , or of modulation of multidrug resistance [9] are carried out using these models.…”

Section: Introductionmentioning

confidence: 99%

Successful Establishment of an Orthotopic Hepatoblastoma In Vivo Model in NOD/LtSz-scid IL2Rγnull Mice

et al. 2011

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Investigation of hepatoblastoma in experimental conditions contributes relevantly to a detailed understanding of tumor biology and the investigation of new treatment approaches. Most systematical analyses currently use subcutaneous xenografts. We established a reproducible intrahepatic model with the hepatoblastoma-cell lines HuH6 and HepT1. The cells were stably transfected with a plasmid vector encoding for Gaussia luciferase. HuH6 and HepT1 were injected intrasplenically in NOD/LtSz-scid IL2Rγnull mice. Mice were splenectomized in order to avoid intrasplenical tumor growth. Multifocal intrahepatic tumor growth was observed in 85% (11/13) of HuH6 tumors and 55% (5/9) of HepT1 tumors. Serum Alpha-fetoprotein and Gaussia luciferase increased 5 weeks after tumor-cell inoculation. Tumors were detected by MRI at this time point. Immunhistochemical analysis such as vascularity (CD31), proliferation index (Ki-67), cytokeratin 7 and distribution of β -catenin in intrahepatic tumors were different to subcutaneous tumors. We established a reproducible xenograft model for intrahepatic hepatoblastoma growth with a high tumor incidence. Monitoring of tumor cell viability was optimized by measuring GLuc. This model enables further experimental investigations of HB in a more physiological milieu as emphasized by the β-catenin distribution.

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“…The aggressive nature of high-risk HB concomitant with the relatively low overall disease incidence has historically prevented substantial study of more effective chemotherapy regimens and targeted therapies due to the lack of available samples. Only three cell lines, HepG2, Huh-6, and HepT1, are widely available for laboratory studies [13][14][15][16]. Relevant animal models have been developed more recently as a response to this historical difficulty, allowing researchers to accurately recapitulate human tumors in murine models to further study tumor biology and new treatment algorithms in an effort to increase overall survival of patients with high-risk disease.…”

Section: Introductionmentioning

confidence: 99%

Animal Modeling of Pediatric Liver Cancer

Whitlock

Yang

Vasudevan

et al. 2020

Cancers

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Hepatoblastoma (HB) is the most common pediatric liver malignancy. Management of HB requires multidisciplinary efforts. The 5-year overall survival of this disease is about 80% in developed countries. Despite advances in the care of these patients, survival in recurrent or treatment-refractory disease is lower than 50%. This is due to more complex tumor biology, including hepatocellular carcinoma (HCC)-like mutations and expression of aggressive gene signatures leading to chemoresistance, vascular invasion, and metastatic spread. The current treatment protocols for pediatric liver cancer do not incorporate targeted therapies, and the ability to test these therapies is limited due to the inaccessibility of cell lines and mouse models. In this review, we discuss the current status of preclinical animal modeling in pediatric liver cancer, primarily HB. Although HB is a rare cancer, the research community has worked together to develop a range of interesting and relevant mouse models for diverse preclinical studies.

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Production of fibronectin by HUH6 Cl5 cell line established from a human hepatoblastoma

Cited by 10 publications

References 11 publications

Use of human-derived liver cell lines for the detection of environmental and dietary genotoxicants; current state of knowledge

Use of human-derived liver cell lines for the detection of environmental and dietary genotoxicants; current state of knowledge

Successful Establishment of an Orthotopic Hepatoblastoma In Vivo Model in NOD/LtSz-scid IL2Rγnull Mice

Animal Modeling of Pediatric Liver Cancer

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