Production of Fibrinolytic Enzyme from Bacillus amyloliquefaciens by Fermentation of Chickpeas, with the Evaluation of the Anticoagulant and Antioxidant Properties of Chickpeas

Abstract

To develop safe and cheap thrombolytic agents, a fibrinolytic enzyme productive strain of LSSE-62 was isolated from Chinese soybean paste. This strain was identified as Bacillus amyloliquefaciens by 16S rDNA sequence analysis. Nucleotide and amino acid sequence analysis showed that this fibrinolytic enzyme was identical to subtilisin DJ-4. Chickpeas were used as the substrate for fibrinolytic enzyme production from B. amyloliquefaciens in solid-state fermentation. Under the optimized conditions (34 °C and 50% initial moisture content), the fibrinolytic activity of fermented chickpeas reached 39.28 fibrin degradation units (FU)/g. Additionally, the fermented chickpeas showed anticoagulant activity, and the purified anticoagulant component showed higher anticoagulant activity than heparin sodium. After fermentation, the total phenolic and total flavonoid contents increased by 222 and 71%, respectively, and then the antioxidant activities were improved significantly. This study provided a novel method for the preparation of multifunctional food of chickpeas or raw materials for the preparation of functional food additives and potential drugs.

Stepwise modifications of genetic parts reinforce the secretory production of nattokinase in Bacillus subtilis

“…An array of NK‐producing Bacillus strains was originally screened for producing NK under naturally fermented conditions (Inatsu et al ., ; Wang et al ., ; Wei et al ., ; Kumar et al ., ). In recent years, along with the cloning of the aprN gene from Bacillus natto, NK has been overexpressed in a variety of hosts, including Bacillus subtilis , Escherichia coli and Lactococcus lactis (Liang et al ., ,b; Chen et al ., ; Degering et al ., ; Nguyen et al ., ; Guan et al ., ,b).…”

Novel Sequential Screening and Enhanced Production of Fibrinolytic Enzyme byBacillussp. IND12 Using Response Surface Methodology in Solid-State Fermentation

“…The antibiotic, Cephamycin C, was produced in SSF using wheat straw, deoiled cake of cotton seed, sunflower cake, and corn steep liquor [18] and wheat bran and sweet lemon peel was used as the substrate for the production of nigerloxin in SSF [19]. Apple pomace and chickpeas were used as the substrate for fibrinolytic enzyme production in SSF by Bacillus cereus GD55 [20] and Bacillus amyloliquefaciens [21]. …”

A low cost fermentation medium for potential fibrinolytic enzyme production by a newly isolated marine bacterium, Shewanella sp. IND20

“…The agro-wastes such as Ficus nitida waste [14], shrimp shell [36], cotton seed cake [6] and corn cob [8] were reported as the substrate for the production of proteolytic enzymes. The substrates such as soybean grits [36], chickpeas [37], wheat bran [35], spent brewery yeast sludge [38] and soybean meal [39] have been already utilized as potential substrates for fibrinolytic enzyme production. Among all substrates, cow dung is a cheap substrate.…”