Production of Fermentable Sugars and Hydrogen-Rich Gas from Agave tequilana Biomass

Farías-Sánchez, Juan Carlos; Velázquez-Valadez, Ulises; Vargas-Santillán, Alfonso; Pineda-Pimentel, María Guadalupe; Mendoza-Chávez, Erick Alejandro; Rutiaga-Quiñones, José Guadalupe; Saucedo-Luna, Jaime; Castro‐Montoya, Agustín Jaime

doi:10.1007/s12155-016-9799-y

Cited by 7 publications

(3 citation statements)

References 12 publications

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“…The genus Agave is cultivated to produce fiber, food, beverages, fuel, etc., and has great socioeconomic and agroecological value, especially in hot and drought‐prone regions of the world; despite its adaptability, these plants suffer from diseases, such as those caused by insects and fungus. Within this genus, Agave tequilana is primarily used for the elaboration of beverages, in soil conservation; furthermore, it has also been indicated that this plant can potentially be an important source of lignocellulosic bioenergy feedstocks .…”

Section: Introductionmentioning

confidence: 99%

A biophysical and structural study of two chitinases from Agave tequilana and their potential role as defense proteins

et al. 2019

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Plant chitinases are enzymes that have several functions, including providing protection against pathogens. Agave tequilana is an economically important plant that is poorly studied. Here, we identified a chitinase from short reads of the A. tequilana transcriptome (AtChi1). A second chitinase, differing by only six residues from the first, was isolated from total RNA of plants infected with Fusarium oxysporum (AtChi2). Both enzymes were overexpressed in Escherichia coli and analysis of their sequences indicated that they belong to the class I glycoside hydrolase family19, whose members exhibit two domains: a carbohydrate‐binding module and a catalytic domain, connected by a flexible linker. Activity assays and thermal shift experiments demonstrated that the recombinant Agave enzymes are highly thermostable acidic endochitinases with Tm values of 75 °C and 71 °C. Both exhibit a molecular mass close to 32 kDa, as determined by MALDI‐TOF, and experimental pIs of 3.7 and 3.9. Coupling small‐angle x‐ray scattering information with homology modeling and docking simulations allowed us to structurally characterize both chitinases, which notably show different interactions in the binding groove. Even when the six different amino acids are all exposed to solvent in the loops located near the linker and opposite to the binding site, they confer distinct kinetic parameters against colloidal chitin and similar affinity for (GlnNAc)6, as shown by isothermal titration calorimetry. Interestingly, binding is more enthalpy‐driven for AtChi2. Whereas the physiological role of these chitinases remains unknown, we demonstrate that they exhibit important antifungal activity against chitin‐rich fungi such as Aspergillus sp. Database SAXS structural data are available in the SASBDB database with accession numbers SASDDE7 and SASDDA6. Enzymes Chitinases (http://www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/14.html).

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Section: Introductionmentioning

confidence: 99%

A biophysical and structural study of two chitinases from Agave tequilana and their potential role as defense proteins

et al. 2019

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“…Therefore, it is necessary to apply speci c treatments to these materials, prior to the hydrolysis and fermentation stages, to modify the chemical and structural composition of the lignocellulosic biomass, improving the access of the hydrolytic agents to cellulose bers [4], [5]. There are several kinds of pre-treatments: physical (crushing, hydrothermolysis) [6], chemical (acid, alkaline, solvents and ozone) [7], physicochemical (steam explosion, ammonium ber explosion) [8] and biological [9], or a combination of these [10] .The choice of the treatment to be applied depends on the biomass to be treated Farias [11].…”

Section: Introductionmentioning

confidence: 99%

Effects of pretreatments on the bioavailability of sugars on discarded melons to obtain bioethanol.

Montoro¹,

Herrero²,

Mamaní³

et al. 2020

Preprint

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Melon is one of the main horticultural crops in the province of San Juan, Argentina. An excess of 20% of its production is not marketed for various reasons. To take advantage of this biomass, acid pretreatments (PA), acid combined with ultrasound (PAU), were applied to evaluate increases in the bioavailability of sugars for the production of 2G bioethanol. The results showed that under the optimal conditions of both pretreatments: concentration of 2% sulfuric acid, time of 30 minutes, the temperature of 55 °C and solid: liquid ratio11:1, followed by enzymatic hydrolysis using cellulase, hemicellulose and pectinase, increases of 111 and 576% are achieved in the content of fermentable sugars, compared to untreated melon. Acid pretreatment combined with ultrasound and enzymatically hydrolyzed, using cellulase, hemicellulase, and pectinase, turned out to be the best alternative in terms of increasing fermentable sugars, obtaining 38.99 mL of bioethanol per kg of melon.

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“…The concentration of ethanol was 30.8 g/L, representing 78.5% of the theoretical maximum. Farías-Sánchez et al [18] studied the production of fermentable sugars and hydrogenrich gas from Agave tequilana biomass. They performed a process simulation to determine the optimum process and operating conditions for effective production of H 2 .…”

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Bioenergy Potential, Energy Crops, and Biofuel Production in Mexico

2016

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The negative impact of burning fossil fuels on the global climate, uncertainty over the long-term price of fossil fuelscaused in part by geopolitics and pending decisions on the exploration of oil shale and gas, tar sands and arctic oil reserves, and in part by uncertainty over future regulations on greenhouse gas emissions-and the increasing global demand for energy resulting from population growth and economic development drive the need to deploy renewable energy on a large scale. Mexico is a promising country in the development of renewable energy due to its warm and sunny climate, which supports solar energy generation and crop cultivation throughout the year, and its relative abundance of agricultural land that is suitable for energy crops, but not for food crops, thus minimizing competition between food and energy production. With a well developed infrastructure of roads, ports, and industrial centers, and a relatively low cost of labor, Mexico is among the most relevant emerging bioeconomies.In a recent study, Alemán-Nava et al.[1] reported the current use and future potential of renewable sources in the production of renewable energy, which included solar, hydroelectric, geothermal and wind energy, and bioenergy. However, these alternative sources of energy have not yet been fully exploited in Mexico. According to the Mexican Ministry of Energy (SENER) and the National Energy Balance databases [2], in 2014, Mexico produced 8826 PetaJoule (PJ = 10 15

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Production of Fermentable Sugars and Hydrogen-Rich Gas from Agave tequilana Biomass

Cited by 7 publications

References 12 publications

A biophysical and structural study of two chitinases from Agave tequilana and their potential role as defense proteins

A biophysical and structural study of two chitinases from Agave tequilana and their potential role as defense proteins

Effects of pretreatments on the bioavailability of sugars on discarded melons to obtain bioethanol.

Bioenergy Potential, Energy Crops, and Biofuel Production in Mexico

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