Production of feather hydrolysate by Elizabethkingia meningoseptica KB042 (MTCC 8360) in submerged fermentation

Abstract

A keratinolytic bacterium Elizabethkingia meningoseptica KB042 was isolated from dropped off feathers. The bacterium showed 82.50 ± 0.3% feather degradation when grown on medium containing 10 g/l chicken feathers with initial pH 7.0 at 37°C, 150 rpm in 6 days. The pH of the medium was increased up to 10.02 ± 0.10 during 6 days of incubation. Soluble protein and amino acids concentration in the culture fluid was also found increased until the end of incubation. During the cultivation of strain KB042 on feather as sole source of carbon and nitrogen, the maximum cysteine release was noted on the 3rd day. Varying feather concentration 1.0-2.0% in basal medium resulted in soluble protein release between 1814.42 and 1954.61 μg/ml. The amino acid concentration was found to be maximum, i.e. 937.85 ± 11.9 μg/ml in the cultures grown with 2% feather. The hydrolysate was also found rich in essential amino acids valine, tryptophan, threonine, leucine and cysteine and contains minor amount of methionine and arginine. These data indicate a potential biotechnology for biotransformation and utilization of feather keratin as a source of protein which can be used as animal feed after successful animal trials.

Production of alkaline protease from Elizabethkingia meningoseptica KB042 using chicken feathers

“…Previously, we isolated and characterized a featherdegrading bacterium, Elizabethkingia meningoseptica KB042, from dropped off feathers (Nagal and Jain 2010a). In the present study, we describe the production and some properties of the alkaline protease from E. meningoseptica KB042 using chicken feathers as a growth substrate.…”

“…Two millilitres of a 24-h-old bacterial culture grown on nutrient broth at 37°C and 150 rpm was used as inoculum (2% v/v). The flasks were then incubated at 37°C, 150 rpm for 6 days (Nagal and Jain 2010a).…”

Isolation, Identification and Characterization of Keratin degrading microorganisms from Poultry soil and their Feather degradation Potential

“…Keratinolytic enzymes are widespread in nature and are produced by several microorganisms including bacteria such as Bacillus sp. [3][4][5][6][7][8], Fervidobacterium islandicum [9], Elizabethkingia meningoseptica KB042 [10], Pseudomonas aeruginosaKS1 [11] and Actinomycetes such as Streptomyces sp. [12][13][14] and fungi such as Chrysosporium tropicum [15], Trichoderma atroviridae [16], Doratomyces microsporus [17]; Paecilomyces marquandii [18]; Scopulariopsis brevicaulis [19]; Alternaria, Paecilomyces, Penicillium, Curvularia and several Aspergillus sp.…”

A Statistical-Mathematical Model to Optimize Chicken Feather Waste Bioconversion via Bacillus licheniformis SHG10: A Low Cost Effective and Ecologically Safe Approach

“…In this respect, microbial feather biodegradation is considered the substantial solution to overcome all the drawbacks encountered in traditional methods for feather recycling. The up to date literature contains plethora of feather-degrading microorganisms synonymously called keratinase-producing microorganisms and their keratinolytic enzymes belonging to bacteria ,actinomycetes in majority and fungi in minority [5,7,[16][17][18][19][20][21][22][23]. Seeking for novel microbes with promising feather-degrading capabilities is currently the main concern of numerous researches in this respect [24][25][26][27][28].…”