Production of Fatty Acid Ethyl Esters from Waste Cooking Oil Using Novozym 435 in a Solvent-Free System

Kochepka, Débora Merediane; Dill, Laís Pastre; Couto, Gustavo Henrique; Krieger, Nádia; Ramos, Luiz Pereira

doi:10.1021/acs.energyfuels.5b02116

Cited by 23 publications

(16 citation statements)

References 33 publications

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“…For the accomplishment of the experiments, an experimental design was performed, evaluating the molar ratio (FFAs/alcohol), mass of biocatalyst, temperature and reaction time [101].…”

Section: Design Of Experimentsmentioning

confidence: 99%

Optimization of the Production of Enzymatic Biodiesel from Residual Babassu Oil (Orbignya sp.) via RSM

et al. 2020

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Residual oil from babassu (Orbignya sp.), a low-cost raw material, was used in the enzymatic esterification for biodiesel production, using lipase B from Candida antarctica (Novozym® 435) and ethanol. For the first time in the literature, residual babassu oil and Novozym® 435 are being investigated to obtain biodiesel. In this communication, response surface methodology (RSM) and a central composite design (CCD) were used to optimize the esterification and study the effects of four factors (molar ratio (1:1–1:16, free fatty acids (FFAs) /alcohol), temperature (30–50 °C), biocatalyst content (0.05–0.15 g) and reaction time (2–6 h)) in the conversion into fatty acid ethyl esters. Under optimized conditions (1:18 molar ratio (FFAs/alcohol), 0.14 g of Novozym® 435, 48 °C and 4 h), the conversion into ethyl esters was 96.8%. It was found that after 10 consecutive cycles of esterification under optimal conditions, Novozym® 435 showed a maximum loss of activity of 5.8%, suggesting a very small change in the support/enzyme ratio proved by Fourier Transform Infrared (FTIR) spectroscopy and insignificant changes in the surface of Novozym® 435 proved by scanning electron microscopy (SEM) after the 10 consecutive cycles of esterification.

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“…For the accomplishment of the experiments, an experimental design was performed, evaluating the molar ratio (FFAs/alcohol), mass of biocatalyst, temperature and reaction time [101].…”

Section: Design Of Experimentsmentioning

confidence: 99%

Optimization of the Production of Enzymatic Biodiesel from Residual Babassu Oil (Orbignya sp.) via RSM

et al. 2020

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“…Matérias graxas utilizadas repetidamente em processos de fritura por imersão sofrem degradação por reações hidrolíticas e oxidativas que as tornam inadequadas para o processamento de alimentos. [27][28][29] Nesse caso, a oxidação, que é acelerada pela alta temperatura do processo, é a principal responsável pela modificação das características físico-químicas e organolépticas do óleo. O óleo torna-se escuro, viscoso, tem sua acidez aumentada e desenvolve odor desagradável, comumente chamado de ranço.…”

Section: Matérias-primas Para a Produção De Biodieselunclassified

“…Isto pode ser atingido pelo isolamento de novos micro-organismos produtores de lipases, por técnicas de melhoramento das propriedades de lipases já existentes ou ainda pela produção de lipases recombinantes com altas atividades e estabilidades nos meios de reação. 28,101,[126][127][128]…”

Section: Produção De Biodiesel Por Catálise Enzimáticaunclassified

Biodiesel: Raw Materials, Production Technologies and Fuel Properties

Ramos

2017

Rev. Virtual Quim.

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The entire biodiesel production chain developed tremendously in regions all over the world and this biofuel has established itself as one of the main alternatives to replace petroleumderived fuels. Several studies have already demonstrated its compatibility with diesel oil and the international standards have evolved to ensure safety and satisfaction to the end-users. Although already losing priority in stronger economies such as United States, Germany and France, this production chain is still expanding considerably in emerging economies such as Brazil, where it has been established as a project of great scientific, technological, political and economic interests. In this article, important aspects of the biodiesel production and use are revised in detail, particularly in relation to the availability of suitable raw materials in large scale as well as to the chemistry involved in the technologies available to date for its conversion to biofuels that are fully compatible with diesel fuel applications.

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“…It has been demonstrated that both chemical ways and enzymatic ways can be used for biodiesel conversion with microalgae oil [5][6][7]. Compared with the conventional chemical catalysis, enzymatic ways have shown distinctive features of mild reaction conditions, a low ratio of alcohol to oil and waste-free during the process [8][9][10][11]. Among different forms of biocatalysts, immobilized lipase has been widely investigated for biodiesel preparation, due to the fact that the immobilized lipase can be easily reused and has a good thermal as well as pH stability [12,13].…”

Section: Introductionmentioning

confidence: 99%

Efficient Biodiesel Conversion from Microalgae Oil of Schizochytrium sp.

et al. 2019

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Microalgae oil has been regarded as a promising feedstock for biodiesel production. However, microalgae oil usually contains some non-lipid components, such as pigments. Microalgae oil could be converted to biodiesel effectively with a two-step process to decrease the negative effect caused by by-product glycerol generated in traditional biodiesel production process. Firstly, microalgae oil was hydrolysed to free fatty acids (FFAs) and then FFAs were converted to methyl ester. In this study, the hydrolysis of microalgae oil from Schizochytrium sp. was systematically investigated and microalgae oil could be hydrolysed effectively to FFAs at both non-catalytic and acid-catalytic conditions. The hydrolysis degree of 97.5% was obtained under non-catalytic conditions of 220 °C and a water to oil ratio of 10:1 (w:w). The hydrolysis degree of 97.1% was obtained with the optimized sulphuric acid catalytic conditions of 95 °C, and a ratio of water to oil 3:1. The lipase Novozym435-mediated esterification with the hydrolysed FFAs was explored and a FAME (Fatty Acids Methyl Ester) yield of 95.1% was achieved. The conversion of different FFAs also was compared and the results indicated that lipase Novozym435-mediated methanolysis was effective for the preparation of biodiesel as well as poly unsaturated fatty acids (PUFAs).

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Production of Fatty Acid Ethyl Esters from Waste Cooking Oil Using Novozym 435 in a Solvent-Free System

Cited by 23 publications

References 33 publications

Optimization of the Production of Enzymatic Biodiesel from Residual Babassu Oil (Orbignya sp.) via RSM

Optimization of the Production of Enzymatic Biodiesel from Residual Babassu Oil (Orbignya sp.) via RSM

Biodiesel: Raw Materials, Production Technologies and Fuel Properties

Efficient Biodiesel Conversion from Microalgae Oil of Schizochytrium sp.

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