Production of fat-1 transgenic rats using a post-natal female germline stem cell line

Zhou, Li; Wang, Lei; Kang, Jing; Xie, Wenhai; Li, Xiaoyong; Wu, Changqing; Xu, Bo; Wu, Ji

doi:10.1093/molehr/gat081

Cited by 107 publications

(128 citation statements)

References 58 publications

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“…In combination with inconsistent with previous in paragraph and below localisation, oocyte proteins such as those from the zona pellucida (ZP) family have been detected to specifically identify OSCs and differentiate them from the stem cell precursors of theca or granulosa cells, which would be positive for c-kit detection alone (Bukovsky et al 2004, BristolGould et al 2006, Bukovsky et al 2008, VirantKlun et al 2008, Zou et al 2009, Parte et al 2011, Zhou et al 2014. However, the ZP protein expression has only been detected in growing oocytes or follicular cells (Philpott et al 1987, Sinowatz et al 2001, Lan et al 2004, and therefore the use of this as a marker of OSCs is dubious.…”

Section: Are Data Describing Oscs Comparable Between Species?mentioning

confidence: 94%

“…The claim of post-natal mammalian oogenesis polarised the scientific community, with many studies supporting the presence of OSCs (Bukovsky et al 2005, Johnson et al 2005a, Virant-Klun et al 2008, Niikura et al 2009, Zou et al 2009, Pacchiarotti et al 2010, Parte et al 2011, Zhang et al 2011, White et al 2012, Zhou et al 2014, whereas many others believed the data had been misinterpreted refuting the finding of OSCs (Bristol-Gould et al 2006, Liu et al 2007, Byskov et al 2011, Kerr et al 2012, Zhang et al 2012, Lei and Spradling 2013, Yuan et al 2013 and required further evidence before believing the existence of OSCs and the idea that this work could be extended to other mammals, including humans (Telfer 2004). However, it is questionable whether studies using alternate species can be used to refute Tilly's claims in mice.…”

Section: A New Eramentioning

confidence: 99%

“…Studies have used mathematical, histological and ovary transplant methods to conclude that OSCs are actively dividing and undergoing meiosis in normal ovulating ovaries to maintain follicle numbers (Johnson et al 2004, Bukovsky et al 2005, Niikura et al 2009, Zou et al 2009, Parte et al 2011, Zhang et al 2011, White et al 2012, Zhou et al 2014. However, other studies, that have not found actively dividing germ cells, suggest a quiescent population of cells (Kerr et al 2006, Liu et al 2007, Kerr et al 2012, Zhang et al 2012, Lei & Spradling 2013, Yuan et al 2013.…”

Section: Do Quiescent Oscs Exist?mentioning

confidence: 99%

“…Histological ovary analyses and mating studies were performed to determine the outcome of the transplanted OSCs and their ability to restore fertility. Transplanted GFP-OSCs have not only gone on to develop into oocytes and form follicles, but have also been successfully fertilised to form transgenic offsprings, as assessed by expression of GFP (Zhang et al 2011, Zhou et al 2014. This method would provide the most convincing evidence yet for a population of OSCs that can develop into oocytes and produce offspring.…”

Section: Do Quiescent Oscs Exist?mentioning

confidence: 99%

“…The majority of studies use feeder cells, which are typically inactivated fibroblasts, to initially culture OSCs (Virant-Klun et al 2008, Zou et al 2009, Zhang et al 2011, Zhang et al 2012, Zhou et al 2014. No information is provided about potential interactions between the cultured cells and the feeder cells, or factors released by the feeder cells.…”

Section: What Are the Effects Of External Factors On Oscs?mentioning

confidence: 99%

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Oocyte stem cells: fact or fantasy?

Horan¹,

Williams²

2017

Reproduction

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For many decades, the dogma prevailed that female mammals had a finite pool of oocytes at birth and this was gradually exhausted during a lifetime of reproductive function. However, in 2004, a new era began in the field of female oogenesis. A study was published that appeared to detect oocyte-stem cells capable of generating new eggs within mouse ovaries. This study was highly controversial and the years since this initial finding have produced extensive research and even more extensive debate into their possibility. Unequivocal evidence testifying to the existence of oocyte-stem cells (OSCs) has yet to be produced, meanwhile the spectrum of views from both sides of the debate are wide-ranging and surprisingly passionate. Although recent studies have presented some convincing results that germ cells exist and are capable of creating new oocytes, many questions remain. Are these cells present in humans? Do they exist in physiological conditions in a dormant state? This comprehensive review first examines where and how the dogma of a finite pool was established, how this has been challenged over the years and addresses the most pertinent questions as to the current status of their existence, their role in female fertility, and perhaps most importantly, if they do exist, how can we harness these cells to improve a woman's oocyte reserve and treat conditions such as premature ovarian insufficiency (POI: also known as premature ovarian failure, POF).Reproduction (2017) 154 R23-R35

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Section: Are Data Describing Oscs Comparable Between Species?mentioning

confidence: 94%

Section: A New Eramentioning

confidence: 99%

Section: Do Quiescent Oscs Exist?mentioning

confidence: 99%

Section: Do Quiescent Oscs Exist?mentioning

confidence: 99%

Section: What Are the Effects Of External Factors On Oscs?mentioning

confidence: 99%

See 3 more Smart Citations

Oocyte stem cells: fact or fantasy?

Horan¹,

Williams²

2017

Reproduction

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Isolation and in vitro culture of ovarian stem cells in Chinese soft‐shell turtle (Pelodiscus sinensis)

Zhu

et al. 2018

J of Cellular Biochemistry

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Gonadal cell lines provide valuable tools for studying gametogenesis, sex differentiation, and manipulating germ cells in reproductive biology. Female germline stem cells have been characterized and isolated from ovaries of mammalian species, including mice and human, but there has been very few studies on female germline stem cells in reptiles. Here, we described an ovarian stem cell-like line isolated and cultured from the Chinese soft-shell turtle (Pelodiscus sinensis), designated as PSO1. The cells showed high alkaline phosphatase activity with a normal diploid karyotype. As shown by reverse transcription-polymerase chain reaction, the cells were positive for the expression of germ cell-specific genes, vasa and dazl, as well as a stem cell marker, nanog, but negative for the expression of the folliculogenesis-specific gene, figla. Likewise, through fluorescent immunostaining analyses, both the Dazl and Vasa proteins were detected abundantly in the cytoplasm of perinuclear region, whereas Nanog and PCNA were dominantly observed in the nuclei in PSO1 cells. Moreover, PSO1 cells transfected with pCS2:h2b-egfp could properly express the fusion protein in the nuclei. Taken together, the findings suggested that the germline stem cells exist in the ovary of juvenile Chinese soft-shell turtle and these cells can be isolated for a long-term in vitro culture under experimental conditions. This study has provided a valuable basis for further investigations on the molecular mechanisms whereby the germline stem cells develop and differentiate into gametes in turtles. Also, it has paved the way for studies on oogenesis in turtles, even in the other reptiles.

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Embryonic Stem Cells and Fetal Development Models

Nowak‐Imialek¹,

Niemann²

2016

Fetal Stem Cells in Regenerative Medicine

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Production of fat-1 transgenic rats using a post-natal female germline stem cell line

Cited by 107 publications

References 58 publications

Oocyte stem cells: fact or fantasy?

Oocyte stem cells: fact or fantasy?

Isolation and in vitro culture of ovarian stem cells in Chinese soft‐shell turtle (Pelodiscus sinensis)

Embryonic Stem Cells and Fetal Development Models

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