Production of Extracellular 5-Aminolevulinic Acid by Rhodopseudomonas Palustris in Solid-State Fermentation

Ong, Pei Ying; Lee, C. T.; Sarmidi, M. R.; Awad, Hassan M.; Chua, Lee Suan; Razali, Firdausi

doi:10.1007/978-1-4614-6208-8_22

Cited by 2 publications

(2 citation statements)

References 10 publications

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“…An extremely active enzyme can catalyze the reaction more rapidly as well as transform the substrates to products more efficiently. 5-Aminolevulinic acid synthase (ALAS), a key enzyme of the C4 pathway, is encoded by hem A gene from different organisms, such as Agrobacterium radiobacter (Fu et al 2010 ), Bradyrhizobium japonicum (Choi et al 1999 ; Jung et al 2004 ), Rhodobacter capsulatus (Kang et al 2004 ; Lou et al 2014 ; Yu et al 2020 ) , Paracoccus (Sato et al 1985 ), Rattus norvegicus (Nakakuki et al 1980 ), Rhodobacter sphaeroides (Kang et al 2011a , b ; Shih et al 2021 ; Tan et al 2020a ; Tran et al 2019 ), Rhodopseudomonas palustris (Ong et al 2013 ; Zhang et al 2013 ; Liu et al 2016 ), and Saccharomyces cerevisiae (Volland and Felix 1984 ). The ALAS from different organisms used for 5-ALA production via C4 pathway in the genetic E. coli strains are summarized in Table 1 .…”

Section: Production Of 5-ala Via C4 Pathway In Escherichia ...mentioning

confidence: 99%

Challenges and opportunities of bioprocessing 5-aminolevulinic acid using genetic and metabolic engineering: a critical review

Shih

et al. 2021

Bioresour. Bioprocess.

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Abstract5-Aminolevulinic acid (5-ALA), a non-proteinogenic five-carbon amino acid, has received intensive attentions in medicine due to its approval by the US Food and Drug Administration (FDA) for cancer diagnosis and treatment as photodynamic therapy. As chemical synthesis of 5-ALA performed low yield, complicated processes, and high cost, biosynthesis of 5-ALA via C4 (also called Shemin pathway) and C5 pathway related to heme biosynthesis in microorganism equipped more advantages. In C4 pathway, 5-ALA is derived from condensation of succinyl-CoA and glycine by 5-aminolevulic acid synthase (ALAS) with pyridoxal phosphate (PLP) as co-factor in one-step biotransformation. The C5 pathway involves three enzymes comprising glutamyl-tRNA synthetase (GltX), glutamyl-tRNA reductase (HemA), and glutamate-1-semialdehyde aminotransferase (HemL) from α-ketoglutarate in TCA cycle to 5-ALA and heme. In this review, we describe the recent results of 5-ALA production from different genes and microorganisms via genetic and metabolic engineering approaches. The regulation of different chassis is fine-tuned by applying synthetic biology and boosts 5-ALA production eventually. The purification process, challenges, and opportunities of 5-ALA for industrial applications are also summarized.

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Section: Production Of 5-ala Via C4 Pathway In Escherichia ...mentioning

confidence: 99%

Challenges and opportunities of bioprocessing 5-aminolevulinic acid using genetic and metabolic engineering: a critical review

Shih

et al. 2021

Bioresour. Bioprocess.

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“…The solution was pink in color after incubation. The concentration of ALA was detected by taking the absorbance at 556 nm using a visible spectrophotometer (Spectronic 200, USA) (Ong et al 2013).…”

Section: Determination Of Organic Acids and 5-aminolevulinic Acidmentioning

confidence: 99%

Physicochemical profile of microbial-assisted composting on empty fruit bunches of oil palm trees

Lim

Bong

Chua

et al. 2015

Environ Sci Pollut Res

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This study was carried out to investigate the physicochemical properties of compost from oil palm empty fruit bunches (EFB) inoculated with effective microorganisms (EM∙1™). The duration of microbial-assisted composting was shorter (∼7 days) than control samples (2 months) in a laboratory scale (2 kg) experiment. The temperature profile of EFB compost fluctuated between 26 and 52 °C without the presence of consistent thermophilic phase. The pH of compost changed from weak acidic (pH ∼5) to mild alkaline (pH ∼8) because of the formation of nitrogenous ions such as ammonium (NH4 (+)), nitrite (NO2 (-)), and nitrate (NO3 (-)) from organic substances during mineralization. The pH of the microbial-treated compost was less than 8.5 which is important to prevent the loss of nitrogen as ammonia gas in a strong alkaline condition. Similarly, carbon mineralization could be determined by measuring CO2 emission. The microbial-treated compost could maintain longer period (∼13 days) of high CO2 emission resulted from high microbial activity and reached the threshold value (120 mg CO2-C kg(-1) day(-1)) for compost maturity earlier (7 days). Microbial-treated compost slightly improved the content of minerals such as Mg, K, Ca, and B, as well as key metabolite, 5-aminolevulinic acid for plant growth at the maturity stage of compost. Graphical Abstract Microbial-assisted composting on empty fruit bunches.

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Production of Extracellular 5-Aminolevulinic Acid by Rhodopseudomonas Palustris in Solid-State Fermentation

Cited by 2 publications

References 10 publications

Challenges and opportunities of bioprocessing 5-aminolevulinic acid using genetic and metabolic engineering: a critical review

Challenges and opportunities of bioprocessing 5-aminolevulinic acid using genetic and metabolic engineering: a critical review

Physicochemical profile of microbial-assisted composting on empty fruit bunches of oil palm trees

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