Production of exopolysaccharide from ethanol inAgrobacterium radiobacter

Klekner, V.; Симова, Е.; Řičica, J.; Panoš, J.

doi:10.1007/bf02872930

Cited by 2 publications

(2 citation statements)

References 14 publications

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“…shows the effect of reducing the concentration of nitrogen, magnesium and sulphur in X. campestris cultures. The time course cell profile fitting was obtained by the logistic growth curve equation and the xanthan profile fitting by the Luedeking-Piret model(Weiss and Ollis 1980;Klekner et al 1984;Mulchandani et al 1988;Pons …”

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confidence: 99%

Kinetic and metabolic effects of nitrogen, magnesium and sulphur restriction in Xanthomonas campestris batch cultures

Roseiro

Gı́rio

Kará

et al. 1993

Journal of Applied Bacteriology

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By reducing the concentration of nitrogen (from 5.0 to 2.5 mmol 1‐1), batch cultures of Xanthomonas campestris induced the enzyme UDP‐glucose dehydrogenase and stimulated the Entner‐Doudoroff pathway enzyme glucose‐6‐P dehydrogenase. The surplus energy generation was directed to xanthan biosynthesis resulting in a 10% polysaccharide increase. The nitrogen restriction led to a higher consumption of nitrogen (93%) whereas glucose consumption did not surpass 75% utilization. Low concentrations of both magnesium and sulphur exerted a negative effect on xanthan formation. Both restrictions reduced the phosphomannose isomerase enzyme activity by 10‐fold turning the mannose transference presumably into the rate‐limiting step for xanthan biosynthesis. Conversely, the rate of synthesis of glucuronic acid residues did not affect the rate of xanthan biosynthesis. Polysaccharide synthesis in magnesium and sulphur cultures was negatively affected in comparison with cell formation as the cell volumetric production rate increased from 0.037 to 0.091 g 1‐1 h‐1 and the xanthan volumetric production rate dropped from 0.133 g 1‐1 h‐1 to the minimum obtained at 0.083 g 1‐1 h‐1. The efficiency of the carbon substrate conversion was also greatly changed.

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mentioning

confidence: 99%

Kinetic and metabolic effects of nitrogen, magnesium and sulphur restriction in Xanthomonas campestris batch cultures

Roseiro

Gı́rio

Kará

et al. 1993

Journal of Applied Bacteriology

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“…Τελικά ο Klekner et al, (1984) εργάσθηκαν πάνω στην παρα γωγή εξωπολυσακχαριδίου από αιθανόλη, από τον Agrobacterium radiobacter και βρήκαν ότι το βακτήριο αυτό παράγει ένα εζωκυτταρικό πολυσακχαρίδιο από αιθανόλη σε ένα minimal μέσο που περιέ χει νιτρικό άλας σαν αποκλειστική πηγή αζώτου. Όταν το Α. radiobacter καλλιεργείται απουσία CaC0" , μικρό μόνο ποσοστό αιθανόλης μετατρέπεται σε εξωπολυσακχαρι διο.…”

Section: εισαγωγηunclassified

Φύση και συνθήκες παραγωγής πολυσακχαρίδιων από μύκητες του γένους cercospora

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Production of exopolysaccharide from ethanol inAgrobacterium radiobacter

Cited by 2 publications

References 14 publications

Kinetic and metabolic effects of nitrogen, magnesium and sulphur restriction in Xanthomonas campestris batch cultures

Kinetic and metabolic effects of nitrogen, magnesium and sulphur restriction in Xanthomonas campestris batch cultures

Φύση και συνθήκες παραγωγής πολυσακχαρίδιων από μύκητες του γένους cercospora

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