Production of ethanol from mannitol by the yeast strain Saccharomyces paradoxus NBRC 0259

Ota, Anri; Kawai, Shigeyuki; Oda, Hiroshi; Iohara, Koichiro; Murata, Kousaku

doi:10.1016/j.jbiosc.2013.03.018

Cited by 28 publications

(38 citation statements)

References 22 publications

(32 reference statements)

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“…The growth of MK3619 and MK3683 on SM plates was dependent on functional mitochondria and oxygen (see Fig. S1 in the supplemental material), confirming that assimilation of mannitol requires respiration, as reported previously (11,13).…”

Section: Resultssupporting

confidence: 87%

“…We recently reported that S. paradoxus NBRC0259-3 is the most suitable yeast strain for the production of ethanol from mannitol and is superior in this regard to two other microbes, P. angophorae and E. coli KO11 (11). Therefore, we compared the salt tolerances of MK4416 and MK4456 strains with that of S. paradoxus NBRC0259-3, P. angophorae, and E. coli KO11.…”

Section: Resultsmentioning

confidence: 99%

“…P. angophorae (CBS5830) (8) was purchased from CBS-KNAW Fungal Bio-diversity Centre. S. paradoxus strain NBRC0259-3 was obtained previously (11).…”

Section: Methodsmentioning

confidence: 99%

“…Cells were precultured for 24 h at 95 spm, washed once with SDW, resuspended in SDW, and inoculated into 50 ml of YPD or YPM medium in a 100-ml Erlenmeyer flask at an OD 600 of 0.1. To measure the salt tolerance, strains were grown in 1 ml of YPD medium (E. coli KO11 was grown in LBD medium [11] instead) in a test tube with or without 1 M NaCl for 1 day at 145 spm, and the OD 600 of each culture was measured. In the case of flocculated cells, OD 600 was measured after mixing the culture with 0.1 volumes of 500 mM EDTA.…”

Section: Methodsmentioning

confidence: 99%

“…Yeast is currently considered to have several advantages over ethanologenic bacteria, including high tolerance to ethanol and inhibitory compounds (10). Several yeast strains, such as Pichia angophorae and Saccharomyces paradoxus NBRC0259-3, can produce ethanol from mannitol (8,11). However, compared to the well-characterized model organism Saccharomyces cerevisiae, the host-vector systems of these yeasts are not well equipped, and their genetics and physiologies are poorly defined.…”

mentioning

confidence: 99%

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Acquisition of the Ability To Assimilate Mannitol by Saccharomyces cerevisiae through Dysfunction of the General Corepressor Tup1-Cyc8

Chujo

Yoshida

Ota

et al. 2015

Appl Environ Microbiol

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Saccharomyces cerevisiae normally cannot assimilate mannitol, a promising brown macroalgal carbon source for bioethanol production. The molecular basis of this inability remains unknown. We found that cells capable of assimilating mannitol arose spontaneously from wild-type S. cerevisiae during prolonged culture in mannitol-containing medium. Based on microarray data, complementation analysis, and cell growth data, we demonstrated that acquisition of mannitol-assimilating ability was due to spontaneous mutations in the genes encoding Tup1 or Cyc8, which constitute a general corepressor complex that regulates many kinds of genes. We also showed that an S. cerevisiae strain carrying a mutant allele of CYC8 exhibited superior salt tolerance relative to other ethanologenic microorganisms; this characteristic would be highly beneficial for the production of bioethanol from marine biomass. Thus, we succeeded in conferring the ability to assimilate mannitol on S. cerevisiae through dysfunction of Tup1-Cyc8, facilitating production of ethanol from mannitol. Macroalgae, consisting of green, red, and brown algae, are promising sources of biofuels for several reasons: (i) macroalgae are more productive than land crops; (ii) arable land is not required for algal cultivation, obviating the necessity for irrigation, fertilizer, etc.; and (iii) macroalgae contain no lignin (1-4). Both red and brown algae contain high levels of carbohydrates, and a method for producing biofuel from these carbohydrates would be of tremendous economic and environmental benefit.Brown macroalgae contain up to 33% (wt/wt [dry weight]) mannitol, which is the sugar alcohol corresponding to mannose and a promising carbon source for bioethanol production (1, 5, 6). Although some bacteria, such as Escherichia coli and Zymobacter palmae, can assimilate mannitol, i.e., utilize mannitol and produce ethanol (6, 7), bacteria are generally sensitive to ethanol, as well as, several other growth-inhibitory compounds. Z. palmae and E. coli KO11 can produce ca. 1.3% (wt/vol) and 2.6% (wt/vol) ethanol from 3.8% (wt/vol) and 9.0% (wt/vol) mannitol, respectively; however, both strains are sensitive to 5% (wt/vol) ethanol (8,9). Yeast is currently considered to have several advantages over ethanologenic bacteria, including high tolerance to ethanol and inhibitory compounds (10). Several yeast strains, such as Pichia angophorae and Saccharomyces paradoxus NBRC0259-3, can produce ethanol from mannitol (8, 11). However, compared to the well-characterized model organism Saccharomyces cerevisiae, the host-vector systems of these yeasts are not well equipped, and their genetics and physiologies are poorly defined.Mannitol dehydrogenase is the key enzyme that catalyzes the pyridine nucleotide-dependent oxidation of D-mannitol to Dfructose (12). Despite the existence of genes encoding putative homologs of mannitol dehydrogenase (YEL070W and YNR073C), S. cerevisiae strains, including the S288C reference strain, are unable to assimilate mannitol for growth; a few exceptions exi...

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Section: Resultssupporting

confidence: 87%

Section: Resultsmentioning

confidence: 99%

“…P. angophorae (CBS5830) (8) was purchased from CBS-KNAW Fungal Bio-diversity Centre. S. paradoxus strain NBRC0259-3 was obtained previously (11).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Acquisition of the Ability To Assimilate Mannitol by Saccharomyces cerevisiae through Dysfunction of the General Corepressor Tup1-Cyc8

Chujo

Yoshida

Ota

et al. 2015

Appl Environ Microbiol

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Recent advances in metabolic engineering of Corynebacterium glutamicum as a potential platform microorganism for biorefinery

Baritugo

Kim

David

et al. 2018

Biofuels Bioprod Bioref

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The fermentative production of platform chemicals in biorefineries is a sustainable alternative to current petroleum‐refining processes. Industrial microorganisms, such as Escherichia coli, Saccharomyces cerevisiae, and Corynebacterium glutamicum, have been engineered as microbial cell factories that are able to utilize biomass for the production of value‐added platform chemicals and polymers. Compared to E. coli and S. cerevisiae, C. glutamicum displays weak carbon catabolite repression and can co‐utilize mixed sugars as carbon sources, without any significant growth retardation. Pathways for the utilization of alternative carbon sources, such as d‐xylose and l‐arabinose from lignocellulosic biomass, lactose and galactose from whey, glycerol from biodiesel, and methanol from natural gas refineries, have been evaluated for chemical production. However, the application of C. glutamicum in biorefineries is limited because it does not secrete hydrolases for the efficient utilization of cellulose, xylan, and starch from lignocellulosic and starch biomass. To solve the limitation, C. glutamicum has been engineered for the consolidated bioprocessing of biomass by the heterologous expression of amylolytic and cellulolytic enzymes. Recently, C. glutamicum has been extensively engineered for polyamide monomer production owing to its ability to produce l‐lysine and l‐glutamate. This review summarizes recent advances in the development of C. glutamicum strains that can utilize renewable biomass resources for the production of industrially important chemicals. It highlights recent progress in metabolic engineering for the production of polyamide monomers. © 2018 Society of Chemical Industry and John Wiley & Sons, Ltd

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Microorganisms and Genetic Improvement for First and Second Generation Bioethanol Production

Pereira

Maske

Neto

et al. 2022

Biofuel and Biorefinery Technologies

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Production of ethanol from mannitol by the yeast strain Saccharomyces paradoxus NBRC 0259

Cited by 28 publications

References 22 publications

Acquisition of the Ability To Assimilate Mannitol by Saccharomyces cerevisiae through Dysfunction of the General Corepressor Tup1-Cyc8

Acquisition of the Ability To Assimilate Mannitol by Saccharomyces cerevisiae through Dysfunction of the General Corepressor Tup1-Cyc8

Recent advances in metabolic engineering of Corynebacterium glutamicum as a potential platform microorganism for biorefinery

Microorganisms and Genetic Improvement for First and Second Generation Bioethanol Production

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