Production of ethanol from -arabinose by containing a fungal -arabinose pathway

Richard, Peter; Verho, Ritva; Putkonen, Mikko; Londesborough, John; Penttilä, Merja

doi:10.1016/s1567-1356(02)00184-8

Cited by 105 publications

(74 citation statements)

References 27 publications

(33 reference statements)

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“…Since S. cerevisiae is incapable of fermenting pentoses, suboptimal ethanol production is obtained from hemicellulosic hydrolysates with their high pentose contents . Consequently, metabolic engineering has been used extensively in the past decade to establish and improve catabolism of the pentoses arabinose (Becker and Boles, 2003;Richard et al, 2003), and in particular xylose (Eliasson et al, 2000;HahnHägerdal et al, 2001;Ho et al, 1999). Overexpression of the Pichia stipitis xylose reductase and xylitol dehydrogenase, and of the endogenous xylulokinase, resulted only in limited success, since the achieved xylose fermentation rates and ethanol yields were low in comparison to those achieved on glucose (Eliasson et al, 2000;Ho et al, 1998;Kötter and Ciriacy, 1993;Toivari et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Fermentation performance of engineered and evolved xylose‐fermenting Saccharomyces cerevisiae strains

Sonderegger

Jeppsson

Larsson

et al. 2004

Biotech & Bioengineering

129

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Lignocellulose hydrolysate is an abundant substrate for bioethanol production. The ideal microorganism for such a fermentation process should combine rapid and efficient conversion of the available carbon sources to ethanol with high tolerance to ethanol and to inhibitory components in the hydrolysate. A particular biological problem are the pentoses, which are not naturally metabolized by the main industrial ethanol producer Saccharomyces cerevisiae. Several recombinant, mutated, and evolved xylose fermenting S. cerevisiae strains have been developed recently. We compare here the fermentation performance and robustness of eight recombinant strains and two evolved populations on glucose/xylose mixtures in defined and lignocellulose hydrolysate-containing medium. Generally, the polyploid industrial strains depleted xylose faster and were more resistant to the hydrolysate than the laboratory strains. The industrial strains accumulated, however, up to 30% more xylitol and therefore produced less ethanol than the haploid strains. The three most attractive strains were the mutated and selected, extremely rapid xylose consumer TMB3400, the evolved C5 strain with the highest achieved ethanol titer, and the engineered industrial F12 strain with by far the highest robustness to the lignocellulosic hydrolysate. B

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Section: Introductionmentioning

confidence: 99%

Fermentation performance of engineered and evolved xylose‐fermenting Saccharomyces cerevisiae strains

Sonderegger

Jeppsson

Larsson

et al. 2004

Biotech & Bioengineering

129

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“…Presumably, the reduction of NADPH concentration with NAD + regeneration from NADH could diminish xylitol production [191]. However, this approach seems unlikely to be beneficial, as the expression of transhydrogenase of Azotobacter vinlandii in S. cerevisiae augmented the glycerol and 2-oxoglutarate production and changed the intracellular ratio of (NADH/NAD + ):(NADPH/NADP + ) from 17 to 35 [192], which reflected that the thermodynamic equilibrium of the transhydrogenase reaction was toward NADH production.…”

Section: Xylitol Productionmentioning

confidence: 99%

Bioethanol a Microbial Biofuel Metabolite; New Insights of Yeasts Metabolic Engineering

et al. 2018

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Scarcity of the non-renewable energy sources, global warming, environmental pollution, and raising the cost of petroleum are the motive for the development of renewable, eco-friendly fuels production with low costs. Bioethanol production is one of the promising materials that can subrogate the petroleum oil, and it is considered recently as a clean liquid fuel or a neutral carbon. Diverse microorganisms such as yeasts and bacteria are able to produce bioethanol on a large scale, which can satisfy our daily needs with cheap and applicable methods. Saccharomyces cerevisiae and Pichia stipitis are two of the pioneer yeasts in ethanol production due to their abilities to produce a high amount of ethanol. The recent focus is directed towards lignocellulosic biomass that contains 30-50% cellulose and 20-40% hemicellulose, and can be transformed into glucose and fundamentally xylose after enzymatic hydrolysis. For this purpose, a number of various approaches have been used to engineer different pathways for improving the bioethanol production with simultaneous fermentation of pentose and hexoses sugars in the yeasts. These approaches include metabolic and flux analysis, modeling and expression analysis, followed by targeted deletions or the overexpression of key genes. In this review, we highlight and discuss the current status of yeasts genetic engineering for enhancing bioethanol production, and the conditions that influence bioethanol production.

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“…; Kuyper et al, 2003;van Maris et al, 2007). Arabinose utilization capability has been introduced by adding fungal (Richard et al, 2003) and bacterial (Sedlak and Ho, 2001) arabinose-metabolizing genes. Pentose fermentation in industrial strains of S. cerevisiae such as TMB 3006, TMB 3400 and 424A (LNF-ST), containing heterologous xylose reductase and xylitol dehydrogenase, achieved yields of over 0.4 g ethanol per g sugar (Hahn-Hä gerdal et al, 2007).…”

Section: Ethanol (Ch 3 Ch 2 Oh; C 2 H 6 O)mentioning

confidence: 99%

Microbial conversion of sugars from plant biomass to lactic acid or ethanol

2008

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SummaryConcerns for our environment and unease with our dependence on foreign oil have renewed interest in converting plant biomass into fuels and 'green' chemicals. The volume of plant matter available makes lignocellulose conversion desirable, although no single isolated organism has been shown to depolymerize lignocellulose and efficiently metabolize the resulting sugars into a specific product. This work reviews selected chemicals and fuels that can be produced from microbial fermentation of plant-derived cell-wall sugars and directed engineering for improvement of microbial biocatalysts. Lactic acid and ethanol production are highlighted, with a focus on engineered Escherichia coli.

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Production of ethanol from -arabinose by containing a fungal -arabinose pathway

Cited by 105 publications

References 27 publications

Fermentation performance of engineered and evolved xylose‐fermenting Saccharomyces cerevisiae strains

Fermentation performance of engineered and evolved xylose‐fermenting Saccharomyces cerevisiae strains

Bioethanol a Microbial Biofuel Metabolite; New Insights of Yeasts Metabolic Engineering

Microbial conversion of sugars from plant biomass to lactic acid or ethanol

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