Production of enzymes by a newly isolated Bacillus sp. TMF-1 in solid state fermentation on agricultural by-products: The evaluation of substrate pretreatment methods

Salim, Abdalla Ali; Grbavčić, Sanja; Šekuljica, Nataša Ž.; Stefanović, Andrea B.; Tanasković, Sonja Jakovetić; Luković, Nevena; Knežević‐Jugović, Zorica

doi:10.1016/j.biortech.2016.12.081

Cited by 91 publications

(52 citation statements)

References 36 publications

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“…In the present study B. subtilis strain was cultivated on soybean meal (Sojaprotein, Becej, Serbia) under solid‐state fermentation conditions. The approximate composition of the substrate was given before . Employed strain was initially isolated from cow's milk samples and it was kept as a part of culture collection (Microbiological Laboratory of the Faculty of Technology and Metallurgy, University of Belgrade).…”

Section: Methodsmentioning

confidence: 99%

Enzyme production by solid‐state fermentation on soybean meal: A comparative study of conventional and ultrasound‐assisted extraction methods

Salim

Grbavčić

Šekuljica

et al. 2019

Biotech and App Biochem

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Protease, cellulase, and α-amylase producing Bacillus subtilis strain was cultivated by solid-state fermentation technique using soybean meal as a substrate. The aim of the present study was to establish a highly efficient enzymes' extraction method as a first stage in downstream processing. The conventional extraction procedure was optimized by determining pH, stirring rate, solid/liquid ratio, and time of extraction on enzymes' recoveries from fermented soybean meal. Yields of leached enzymes were compared to the amounts of enzymes that are achieved with ultrasound-assisted extraction (UAE). UAE was established to be superior method for obtaining higher yields of proteases (up to 330 IU) and α-amylases (825 IU), under significantly shorter extraction time and gaining more concentrated product. However, the obtained model predicts that conventional process led to a product with a higher cellulolytic activity (ࣙ7.5 IU).

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Section: Methodsmentioning

confidence: 99%

Enzyme production by solid‐state fermentation on soybean meal: A comparative study of conventional and ultrasound‐assisted extraction methods

Salim

Grbavčić

Šekuljica

et al. 2019

Biotech and App Biochem

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“…The bacteria were maintained on LB agar slants containing (g L −1 ): peptone 10, yeast extract 5, NaCl 10 and agar 15. The following selective media were evaluated to screen bacteria that are capable of producing multienzyme complex (MEC) (Salim et al 2017). Carboxymethyl cellulose (CMC) agar plates (per liter: CMC 10 g, yeast extract 3 g, K 2 HPO 4 3 g, KH 2 PO 4 1 g, MgSO 4 •7H 2 O 0.5 g, and agar 20 g).…”

Section: The Selection and Microorganismmentioning

confidence: 99%

“…However, few studies were done to explore the potential capability of B. velezensis in degrading lignocellulose components and transform lignocelluloses into useful chemicals and fuels. Solid-state fermentation (SSF) has attracted more attention to be a significant technique using agro-industrial wastes and by-products as substrates for microbial growth (Moftah et al 2012;Salim et al 2017), and produce industrially useful enzymes in low-cost processes, reduce costs and broaden the industrial application (Lizardi-Jiménez and Hernández-Martínez 2017; Hashemi et al 2011;Elshishtawy et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Complete genome sequence of Bacillus velezensis 157 isolated from Eucommia ulmoides with pathogenic bacteria inhibiting and lignocellulolytic enzymes production by SSF

Chen

et al. 2018

3 Biotech

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157 was isolated from the bark of , and exhibited antagonistic activity against a broad spectrum of pathogenic bacteria and fungi. Moreover, 157 also showed various lignocellulolytic activities including cellulase, xylanase, α-amylase, and pectinase, which had the ability of using the agro-industrial waste (soybean meal, wheat bran, sugarcane bagasse, wheat straw, rice husk, maize flour and maize straw) under solid-state fermentation and obtained several industrially valuable enzymes. Soybean meal appeared to be the most efficient substrate for the single fermentation of 157. Highest yield of pectinase (19.15 ± 2.66 U g), cellulase (46.69 ± 1.19 U g) and amylase (2097.18 ± 15.28 U g) was achieved on untreated soybean meal. Highest yield of xylanase (22.35 ± 2.24 U g) was obtained on untreated wheat bran. Here, we report the complete genome sequence of the 157, composed of a circular 4,013,317 bp chromosome with 3789 coding genes and a G + C content of 46.41%, one circular 8439 bp plasmid and a G + C content of 40.32%. The genome contained a total of 8 candidate gene clusters (bacillaene, difficidin, macrolactin, butirosin, bacillibactin, bacilysin, fengycin and surfactin), and dedicates over 15.8% of the whole genome to synthesize secondary metabolite biosynthesis. In addition, the genes encoding enzymes involved in degradation of cellulose, xylan, lignin, starch, mannan, galactoside and arabinan were found in the 157 genome. Thus, the study of 157 broadened that can not only be used as biocontrol agents, but also has potentially a wide range of applications in lignocellulosic biomass conversion.

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“…The suitability of using SFC as substrate for SSF was previously reported for filamentous fungi (Jacobs et al, 2010), yeasts mixing SFC with other co-substrate (Jiménez-Peñalver et al, 2016) and bacteria (Salim et al, 2017).…”

Section: Fungal Biomass Total Lipids and Protein Accumulationmentioning

confidence: 61%

Bio-enrichment of oilseed cakes by Mortierella alpina under solid-state fermentation

Ferreira

Fernandes

Perés

et al. 2020

LWT

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Oilseed cakes have potential for new applications as substrates for solid-state fermentation (SSF), to increase their nutritional value by increasing its polyunsaturated fatty acids (PUFAs) or protein content. In this sense, it was performed a screening of oilseed cakes to be used as substrates for the production of PUFAs by Mortierella alpina Peyronel MUM 9412. Of all by-products tested, linseed cake (LSC) was the oilseed cake that achieved the highest production of total PUFAs: 153.09 ± 2.25 mg/g. Overall, the PUFAs and protein contents of fermented LSC increased 33% and 11%, respectively. Further, supplementation of the rapeseed cake with linseed oil, prior to the SSF, proved to increase the PUFAs production in about 26%. This study demonstrated the potential of SSF for improving linseed and linseed cakes nutritional composition and the positive effect of linseed oil as inductor to improve the PUFAs production by M. alpina.

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Production of enzymes by a newly isolated Bacillus sp. TMF-1 in solid state fermentation on agricultural by-products: The evaluation of substrate pretreatment methods

Cited by 91 publications

References 36 publications

Enzyme production by solid‐state fermentation on soybean meal: A comparative study of conventional and ultrasound‐assisted extraction methods

Enzyme production by solid‐state fermentation on soybean meal: A comparative study of conventional and ultrasound‐assisted extraction methods

Complete genome sequence of Bacillus velezensis 157 isolated from Eucommia ulmoides with pathogenic bacteria inhibiting and lignocellulolytic enzymes production by SSF

Bio-enrichment of oilseed cakes by Mortierella alpina under solid-state fermentation

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