Production of Energy and Determination of Competence: Past Knowledge, Present Research, and Future Opportunities in Oocyte and Embryo Metabolism

Herrick, Jason R.; Silva, Elena; Krisher, Rebecca L.

doi:10.1002/9781118881286.ch7

Cited by 1 publication

(1 citation statement)

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“…During oocyte development and maturation, cumulus cells metabolize glucose and transfer the resulting pyruvate or lactate to oocytes via transzonal projections and gap junctions ( Herrick et al, 2017 ). Glucose is generally not considered important for oocyte energy production because of limited phosphofructokinase expression in oocytes when compared to cumulus cells ( Richani et al, 2021 ); this was initially demonstrated in the bovine ( Cetica et al, 2002 ), although species differences may exist.…”

Section: Discussionmentioning

confidence: 99%

Equine maternal aging affects the metabolomic profile of oocytes and follicular cells during different maturation time points

Catandi,

Bresnahan,

Peters

et al. 2023

Front. Cell Dev. Biol.

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Introduction: Oocyte quality and fertility decline with advanced maternal age. During maturation within the ovarian follicle, the oocyte relies on the associated somatic cells, specifically cumulus and granulosa cells, to acquire essential components for developmental capacity.Methods: A nontargeted metabolomics approach was used to investigate the effects of mare age on different cell types within the dominant, follicular-phase follicle at three time points during maturation. Metabolomic analyses from single oocytes and associated cumulus and granulosa cells allowed correlations of metabolite abundance among cell types.Results and Discussion: Overall, many of the age-related changes in metabolite abundance point to Impaired mitochondrial metabolic function and oxidative stress in oocytes and follicular cells. Supporting findings include a higher abundance of glutamic acid and triglycerides and lower abundance of ceramides in oocytes and somatic follicular cells from old than young mares. Lower abundance of alanine in all follicular cell types from old mares, suggests limited anaerobic energy metabolism. The results also indicate impaired transfer of carbohydrate and free fatty acid substrates from cumulus cells to the oocytes of old mares, potentially related to disruption of transzonal projections between the cell types. The identification of age-associated alterations in the abundance of specific metabolites and their correlations among cells contribute to our understanding of follicular dysfunction with maternal aging.

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Section: Discussionmentioning

confidence: 99%