Production of “ectopic” vasoactive intestinal peptide-like immunoreactivity in normal human chromaffin cell cultures

Tischler, Arthur S.; Lee, Ying C.; Perlman, Robert L.; Costopoulos, Donna; Bloom, Stephen R.

doi:10.1016/0024-3205(85)90005-0

Cited by 16 publications

(7 citation statements)

References 17 publications

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“…Over the past 30 years, adrenal medullary tissue has been used to obtain cultures of primary human chromaffin cells for developmental and functional studies with and without digestion before physical separation from the cortex (Tischler and Slayton 1983; Tischler et al 1985; Powers et al 1998, 2009; Cavadas et al 2001) and for therapeutic transplantation in multiple sclerosis (Lopez-Lozano et al 1989) and chronic pain patients (Lazorthes et al 1995; Sagen et al 1993). In some older publications, the identity of the collected cells was not verified (e.g., Saria et al 1980; Corder and Lowry 1982; Gaspar et al 1989).…”

Section: Resultsmentioning

confidence: 99%

“…In some older publications, the identity of the collected cells was not verified (e.g., Saria et al 1980; Corder and Lowry 1982; Gaspar et al 1989). Other authors have verified chromaffin cell origin by testing the response to acetylcholine in vitro (Grazzini et al 1999), by measuring catecholamine levels (Evans et al 1983), by testing for TH immunoreactivity (Tischler and Slayton 1983; Tischler et al 1985; Powers et al 1998, 2009; Cavadas et al 2001; Lazorthes et al 1995; Bés et al 1998), or by labeling with neutral red and/or the catecholamine fluorescence technique (Hansen et al 1988; Crickard et al 1982; Lopez-Lozano et al 1989). Most of these approaches are well suited for cultured cells but are not practical for tissue extracts.…”

Section: Resultsmentioning

confidence: 99%

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Tyrosine hydroxylase, chromogranin A, and steroidogenic acute regulator as markers for successful separation of human adrenal medulla

Fliedner¹,

Breza²,

Květňanský³

et al. 2010

Cell Tissue Res

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Progress in high throughput “-omic” techniques now allows the simultaneous measurement of expression levels of thousands of genes and promises the improved understanding of the molecular biology of diseases such as cancer. Detection of the dysfunction of molecular pathways in diseases requires healthy control tissue. This is difficult to obtain from pheochromocytomas (PHEOs), rare chromaffin tumors derived from adrenal medulla. The two options for obtaining adrenal tissue are: (1) whole organ removal post-mortem or during radical nephrectomy; (2) removal during PHEO surgery. Access to high quality normal adrenal tissue is limited. Removal of whole adrenals during nephrectomy is rare, because of improved surgical techniques. For adrenals removed post-mortem, the lag time to proper organ perfusion causes uncontrolled tissue degradation. Adjacent normal adrenal tissue can almost never be obtained from resected PHEOs, because they often replace the entire medulla or are well-encapsulated. If a margin of normal adrenal is attached to a resected PHEO, it seldom contains any medulla. The clean separation of medulla and cortex is further complicated, because their border is convoluted, and because adult adrenal consists of ~90% cortex. Thus, the quality of separation has to be evaluated with specific medullary and cortical markers. We describe the successful dissection of highly pure, medullary tissue from adrenals snap-frozen upon resection during radical nephrectomy or after brain death. Separation quality has been verified by quantitative reverse transcription with polymerase chain reaction for the medullary enzymes, tyrosine hydroxylase, and chromogranin A, and for the cortical enzyme, steroidogenic acute regulator.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Tyrosine hydroxylase, chromogranin A, and steroidogenic acute regulator as markers for successful separation of human adrenal medulla

Fliedner¹,

Breza²,

Květňanský³

et al. 2010

Cell Tissue Res

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“…Subsequent studies showed that normal human chromaffin cells in the adult stage showed a similar response to NGF, developing processes but not in the presence of dexamethasone (Tischler et al 1980), and were also electrically excitable ). In addition, up regulation of neuron-specific or ectopic neuropeptides (VIP, DA) (Tischler et al 1985) and an increase in the receptor tyrosine kinase RET take place in the presence of NGF (Powers et al 2009). Moreover, adult human chromaffin cells are effectively differentiated by GDNF (Powers et al 1998).…”

Section: Plasticitymentioning

confidence: 98%

Past, Present and Future of Human Chromaffin Cells: Role in Physiology and Therapeutics

2010

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Chromaffin cells are neuroendocrine cells mainly found in the medulla of the adrenal gland. Most existing knowledge of these cells has been the outcome of extensive research performed in animals, mainly in the cow, cat, mouse and rat. However, some insight into the physiology of this neuroendocrine cell in humans has been gained. This review summarizes the main findings reported in human chromaffin cells under physiological or disease conditions and discusses the clinical implications of these results.

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“…This response to NGF is for the most part lost in rat chromaffin cells in adult life under the same culture conditions (13, 86). However, it reappears in rat pheochromocytoma cells (81):-In contrast to those of the rat, adult human chromaffin cells remain plastic both morphologically and functionally throughout life (72,82).…”

Section: Neuroendocrine Systemmentioning

confidence: 99%

The Dispersed Neuroendocrine Cells: The Structure, Function, Regulation and Effects of Xenobiotics on this System

Tischler

1989

Toxicol Pathol

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A B S T R A~Cells which express morphological and functional markers originally described in neurons and neural crest: derived endocrine cells are now known to originate from both ectodermal and endodermal progenitors. These cells are organized to secrete peptides, amines, and other regulatory products in response to neurogenic orchemical stimulation. Individual products may function in endocrine, paracrine, neurotransmitter, or neuromodulatory roles. Multiple products are often produced by individual cells and stored in the same secretory granules. The hormonal profiles of particular types of neuroendocrine cells can, to varying degrees, be changed by environmental signals during development or in adult life. These changes are caused both by transcriptional and post-transcriptional mechanisms. Hormonal profiles are also altered during'the development and progression of neoplasia. Signals which stimulate hormone secretion produce a number of ancillary effects, including activation and induction of enzymes which replenish hormone stores, activation of cellular on-. cogenes, and stimulation of cell proliferation. The effects of environmental signals on neuroendocrine cells are mediated by intracellular transduction pathways which involve cyclic AMP, phosphatidylinositol, calcium, and receptor protein kinase activity. These effects can be potentiated, inhibited, or qualitatively altered by exogenous agents.

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Production of “ectopic” vasoactive intestinal peptide-like immunoreactivity in normal human chromaffin cell cultures

Cited by 16 publications

References 17 publications

Tyrosine hydroxylase, chromogranin A, and steroidogenic acute regulator as markers for successful separation of human adrenal medulla

Tyrosine hydroxylase, chromogranin A, and steroidogenic acute regulator as markers for successful separation of human adrenal medulla

Past, Present and Future of Human Chromaffin Cells: Role in Physiology and Therapeutics

The Dispersed Neuroendocrine Cells: The Structure, Function, Regulation and Effects of Xenobiotics on this System

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