Production of Dunaliella salina biomass rich in 9-cis-β-carotene and lutein in a closed tubular photobioreactor

García-González, Mercedes; Moreno, José; Manzano, J.C.; Florencio, Francisco J.; Guerrero, Miguel G.

doi:10.1016/j.jbiotec.2004.07.010

Cited by 228 publications

(132 citation statements)

References 26 publications

(29 reference statements)

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“…Thus, in summer, the medium temperature was also close to the optimum for the growth of the cyanobacterium (25 to 35°C). Maximal algal biomass productivity in summer was also reported by many researchers (7,11). Table 3 shows the seasonal variation in bioremediation efficiency of A. fertilissima after 15 days of incubation compared with the permissible limits of fish culture ponds, i.e., the water quality needed to support fish (13,38).…”

Section: Resultssupporting

confidence: 53%

Wastewater Utilization for Poly-β-Hydroxybutyrate Production by the Cyanobacterium Aulosira fertilissima in a Recirculatory Aquaculture System

Samantaray

Nayak

Mallick

2011

Appl Environ Microbiol

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Intensive aquaculture releases large quantities of nutrients into aquatic bodies, which can lead to eutrophication. The objective of this study was the development of a biological recirculatory wastewater treatment system with a diazotrophic cyanobacterium, Aulosira fertilissima, and simultaneous production of valuable product in the form of poly-␤-hydroxybutyrate (PHB). To investigate this possible synergy, batch scale tests were conducted under a recirculatory aquaculture system in fiber-reinforced plastic tanks enhanced by several manageable parameters (e.g., sedimentation, inoculum size, depth, turbulence, and light intensity), an adequate combination of which showed better productivity. The dissolved-oxygen level increased in the range of 3.2 to 6.9 mg liter ؊1 during the culture period. Nutrients such as ammonia, nitrite, and phosphate decreased to as low as zero within 15 days of incubation, indicating the system's bioremediation capability while yielding valuable cyanobacterial biomass for PHB production. Maximum PHB accumulation in A. fertilissima was found in sedimented fish pond discharge at 20-cm culture depth with stirring and an initial inoculum size of 80 mg dry cell weight (dcw) liter ؊1 . Under optimized conditions, the PHB yield was boosted to 92, 89, and 80 g m ؊2 , respectively for the summer, rainy, and winter seasons. Extrapolation of the result showed that a hectare of A. fertilissima cultivation in fish pond discharge would give an annual harvest of ϳ17 tons dry biomass, consisting of 14 tons of PHB with material properties comparable to those of the bacterial polymer, with simultaneous treatment of 32,640 m 3 water discharge.Over the last 2 decades, aquaculture has gone through major changes, growing from small-scale homestead level activities to large-scale commercial farming, which is driving the industry toward more intensive practices. One of the major drawbacks of this trend lies in the waste derived from fish feed and its metabolic end products. These include uneaten food, feces and excreta, and dissolved inorganic nutrients, which are transported in water in various concentrations and can cause eutrophication of the water resources that receive them (23). Therefore, to combat this problem, a novel technique, i.e., a recirculatory aquaculture system (RAS), has been proposed, which is based on conservation of the limited water and abatement of water pollution caused by the expanding aquaculture industries. The most prominent characteristic of this system is the presence of a biofilter, which treats water contaminated by dissolved organics and ammonia rather than discharging these contaminants directly into bodies of water.Cyanobacteria (blue-green algae) are O 2 -evolving photosynthesizing prokaryotes that can be successfully cultivated in wastewater due to their ability to use inorganic nitrogen and phosphorus for their multiplication. For example, Spirulina platensis (5, 10), Spirulina maxima (25), Romeria sp. (3), Synechococcus sp. strain PCC 7942 (16), Schizothrix calcicola, Pho...

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Section: Resultssupporting

confidence: 53%

Wastewater Utilization for Poly-β-Hydroxybutyrate Production by the Cyanobacterium Aulosira fertilissima in a Recirculatory Aquaculture System

Samantaray

Nayak

Mallick

2011

Appl Environ Microbiol

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“…The estimated market size for natural β-carotene is 10-100 tons·year -1 and its price is higher than 750 €·kg -1 (Pulz, 2001). Several industrial production plants are operating in Australia, Israel, USA and China (León et al 2003;García-González et al 2005), as well as small plants which are located in other countries such as Chile or Iran (BenAmotz, 2004). …”

mentioning

confidence: 99%

Characterization of Dunaliella salina strains by flow cytometry: a new approach to select carotenoid hyperproducing strains

Mendoza¹,

Jara²,

Freijanes³

et al. 2008

Electron. J. Biotechnol.

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“…The maximal biomass productivity (over 2 g dry wt. m − 2 day − 1 or 80 g − 3 day − 1 ) was achieved (Garcia-Gonzalez et al, 2005). This productivity value is higher than what the same research group carried out in an open pond system demonstrating that the closed tubular photobioreactor has significant advantages over the open ponds in the marine algal yield rate (Garcia-Gonzalez et al, 2003).…”

Section: Photoioreactor For Marine Microalgaementioning

confidence: 69%

Advances in genetic engineering of marine algae

Qin

Lin

Jiang

2012

Biotechnology Advances

139

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Production of Dunaliella salina biomass rich in 9-cis-β-carotene and lutein in a closed tubular photobioreactor

Cited by 228 publications

References 26 publications

Wastewater Utilization for Poly-β-Hydroxybutyrate Production by the Cyanobacterium Aulosira fertilissima in a Recirculatory Aquaculture System

Wastewater Utilization for Poly-β-Hydroxybutyrate Production by the Cyanobacterium Aulosira fertilissima in a Recirculatory Aquaculture System

Characterization of Dunaliella salina strains by flow cytometry: a new approach to select carotenoid hyperproducing strains

Advances in genetic engineering of marine algae

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