Production of dragon's blood in Dracaena cochinchinensis plants by inoculation of Fusarium proliferatum

Wang, Xinghong; Zhang, Changhe; Yang, Ling–Ling; Gomes-Laranjo, José

doi:10.1016/j.plantsci.2010.09.007

Cited by 51 publications

(61 citation statements)

References 19 publications

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“…In this work, we performed transciptome analysis of three samples with different flavonoid accumulation to identify putative genes for flavonoid metabolism in D. cambodiana. As a kind of plant secondary metabolite, the formation of dragon’s blood was considered as a result of plant defense response to biotic and abiotic responses45. Calcium is a critical second messenger in the signal transduction pathways of biotic and abiotic stimuli.…”

Section: Discussionmentioning

confidence: 99%

“…In nature, Dracaena plants grow very slowly, and only trees with 30–50-years-old have the possibility to produce a small amount of dragon’s blood. In addition, natural Dracaena resources have been destroyed severely owing to overexploitation45. Our previous studies found an effective inducer (authorized patent: ZL 201310207182.5) could induce the formation of the resin in the stem of D. Cambodiana 6.…”

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confidence: 99%

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De Novo transcriptome characterization of Dracaena cambodiana and analysis of genes involved in flavonoid accumulation during formation of dragon’s blood

Zhu

Cao

Dai

et al. 2016

Sci Rep

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Dragon’s blood is a red resin mainly extracted from Dracaena plants, and has been widely used as a traditional medicine in East and Southeast Asia. The major components of dragon’s blood are flavonoids. Owing to a lack of Dracaena plants genomic information, the flavonoids biosynthesis and regulation in Dracaena plants remain unknown. In this study, three cDNA libraries were constructed from the stems of D. cambodiana after injecting the inducer. Approximately 266.57 million raw sequencing reads were de novo assembled into 198,204 unigenes, of which 34,873 unique sequences were annotated in public protein databases. Many candidate genes involved in flavonoid accumulation were identified. Differential expression analysis identified 20 genes involved in flavonoid biosynthesis, 27 unigenes involved in flavonoid modification and 68 genes involved in flavonoid transport that were up-regulated in the stems of D. cambodiana after injecting the inducer, consistent with the accumulation of flavonoids. Furthermore, we have revealed the differential expression of transcripts encoding for transcription factors (MYB, bHLH and WD40) involved in flavonoid metabolism. These de novo transcriptome data sets provide insights on pathways and molecular regulation of flavonoid biosynthesis and transport, and improve our understanding of molecular mechanisms of dragon’s blood formation in D. cambodiana.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

De Novo transcriptome characterization of Dracaena cambodiana and analysis of genes involved in flavonoid accumulation during formation of dragon’s blood

Zhu

Cao

Dai

et al. 2016

Sci Rep

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“…S.C.CHEN (12,13). DB is widely used as an external wash to promote healing of wounds and to stop bleeding, and also internally as the "bloodactivating drug" to treat various pains, post partum bleeding, internal traumas, and menstrual irregularities in China (14,15).…”

Section: Introductionmentioning

confidence: 99%

Dragon’s Blood Inhibits Chronic Inflammatory and Neuropathic Pain Responses by Blocking the Synthesis and Release of Substance P in Rats

Wang

Jia

et al. 2012

J Pharmacol Sci

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Abstract. As a traditional Chinese medicine, dragon's blood (DB) is widely used in treating various pains for thousands of years due to its potent anti-inflammatory and analgesic effects. In the present study, we observed that intragastric administration of DB at dosages of 0.14, 0.56, and 1.12 g/kg potently inhibited paw edema, hyperalgesia, cyclooxygenase-2 (COX-2) protein expression, or preprotachykinin-A mRNA expression in carrageenan-inflamed or sciatic nerve-injured (chronic constriction injury) rats, respectively. A short-term (15 s or 10 min) pre-exposure of cultured rat dorsal root ganglion (DRG) neurons to DB (0.3, 3, and 30 μg/ml) or its component cochinchinenin B (CB; 0.1, 1, and 10 μM) blocked capsaicin-evoked increases in both the intracellular calcium ion concentration and the substance P release. Moreover, a long-term (180 min) exposure of cultured rat DRG neurons to DB or CB significantly attenuated bradykinin-induced substance P release. These findings indicate that DB exerts anti-inflammatory and analgesic effects by blocking the synthesis and release of substance P through inhibition of COX-2 protein induction and intracellular calcium ion concentration. Therefore, DB may serve as a promising potent therapeutic agent for treatment of chronic pain, and its effective component CB might partly contribute to anti-inflammatory and analgesic effects.

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“…Zhao et al (2005) indicated the important role of fungi in the production of secondary metabolites against foreign attacks. An example is the promotion of the fungi Fusarium proliferatum and Colletotrichum gloeosporioides in production of dragon's blood in Dracaena cochinchinensis, a rare medicinal plant (Wang et al 2011). While, research on fungus inoculation to A. sinensis trees showed that after fungus invasion to tissues, the host not only retards fungal growth but also interrupts its own nutritional supply by plugging conduction tissue.…”

Section: Discussionmentioning

confidence: 99%

Impacts of biological, chemical and mechanical treatments on sesquiterpene content in stems of planted Aquilaria crassna trees

Thành

Son

et al. 2015

Agroforest Syst

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Agarwood trade has been growing rapidly due to its active ingredients composed of volatile substances, which are widely used in the cosmetics and pharmaceuticals. However, the formation time of agarwood in nature is quite long and little is known about its formation agents and mechanisms in planted Aquilaria crassna trees. In this study, biological, chemical and mechanical treatments were applied to 5-, 8-, and 11-year-old A. crassna plantations in north central, Vietnam. Agarwood samples were collected at 1 and 2 years after treatment. Oil content (O c ) in dry wood of A. crassna and sesquiterpene content in extracted oil were analyzed using hydrodistillation method and gas chromatography-mass spectrometry. The results indicated that 1 year after treatment, control (no treatment) and mechanical treatment (nails hammered to tree stems) had highest O c (0.061-0.079 %) but lowest sesquiterpene content (2.1-7.1 %). Chemical treatment (mixture of acid sulfuric and sodium methyl bisulfate) had lowest O c (0.038-0.039 %) but highest sesquiterpene content (15.8-20.8 %). While, 1 year after treatment biological treatments had O c of 0.050-0.077 % and sesquiterpene content of 2.4-11.1 %. Two years after treatment, control still had lowest sesquiterpene content (3.2-7.0 %), while highest content (13.9-44.2 %) belonged to biological treatment (a mixture of fungi Phialophora spp. and Fusarium spp.). There was a total of 56 sesquiterpenes found in extracted oil from wood samples in biological treatments, which included eight highly commerciallyvaluable sesquiterpenes on international trade. Biological treatment with a mixture of fungi P. spp. and F. spp. should be encouraged to apply to 11-year-old A. crassna plantation, which resulted in highest sesquiterpene content (44.2 %).

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Production of dragon's blood in Dracaena cochinchinensis plants by inoculation of Fusarium proliferatum

Cited by 51 publications

References 19 publications

De Novo transcriptome characterization of Dracaena cambodiana and analysis of genes involved in flavonoid accumulation during formation of dragon’s blood

De Novo transcriptome characterization of Dracaena cambodiana and analysis of genes involved in flavonoid accumulation during formation of dragon’s blood

Dragon’s Blood Inhibits Chronic Inflammatory and Neuropathic Pain Responses by Blocking the Synthesis and Release of Substance P in Rats

Impacts of biological, chemical and mechanical treatments on sesquiterpene content in stems of planted Aquilaria crassna trees

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