Production of doubled haploids in durum wheat (Triticum turgidum L.) through isolated microspore culture

Improvements in the production of doubled haploids in durum wheat (Triticum turgidum L.) through isolated microspore culture

“…Olsen (1987) demonstrated that in barley glutamine can play a key role to increase the proportion of green plants. We have demonstrated that the C 17 medium is a good medium for microspore culture of durum wheat (Cistué et al 2006). In this study, supplementing the C 17 medium with glutamine significantly increased the number of green plants.…”

“…Plates were kept for 5-6 days at 24 ºC in the dark. Microspore isolation was performed according to the durum wheat protocol described by Cistué et al (2006).…”

“…Co-culture with ovaries has been tested and found to be a useful method to increase green plant production in hexaploid wheat microspore culture (Puolimatka et al 1996;Hu and Kasha 1997;Zheng et al 2002). It has also been used for recalcitrant genotypes in barley (Devaux and Li 2001) and shown to be beneficial in durum wheat (J'Aiti et al 1999;Cistué et al 2006).…”

“…The aim of this study was to improve the induction medium developed by Cistué et al (2006) and to optimize the growth conditions of the donor plants. Two new substances were added to the induction medium.…”

“…Additionally, when green plants were obtained (Dogramaci-Altuntepe et al 2001) high numbers of aneuploids were regenerated from the androgenic calli. Cistué et al (2006) developed a technique which produced green plants from a range of different genotypes and 68% were doubled haploid plants. Other works recently have been published although low success was reported (Labbani et al 2005;Bakos et al 2007).…”