Production of doubled haploids in sugar beet (Beta vulgaris): an efficient method by a multivariate experiment

Pazuki, Arman; Aflaki, Fatemeh; Gürel, Songül; Ergül, Ali; Gürel, Ekrem

doi:10.1007/s11240-017-1313-5

Cited by 21 publications

(8 citation statements)

References 37 publications

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“…3). In other pieces of research on sugar beet haploid gynogenesis from in vitro cultured ovules, BAP at 1 or 2 mg L -1 caused a statistically significant amount of hyperhydricity in gynogenic embryos (F (2, 41) = 7.102, p = 0.002) (Pazuki et al 2017a), whereas Kin with a reasonable amount of regeneration did not result in hyperhydricity of the embryos (F (2, 106) = 22.05, p < 0.001) (Pazuki et al 2017b).…”

Section: Resultsmentioning

confidence: 95%

“…A hyperhydric sugar beet subculture on a medium with a new composition or under new conditions takes time and effort to produce a normal plant, which is a costly practice (Tomaszewska-Sowa 2012). The superior effect of kinetin (Kin) over BAP has been observed in sugar beet gynogenesis (Pazuki et al 2017a(Pazuki et al , 2017b. It was recorded that BAP could cause higher hyperhydricity than Kin.…”

Section: Introductionmentioning

confidence: 99%

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A robust method for haploid sugar beet in vitro proliferation and hyperhydricity reduction

Pazuki

Aflaki

Gürel

et al. 2017

Folia Horticulturae

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Sugar beet is recalcitrant to in vitro tissue culture. Usually, proliferation of in vitro cultured rosette explants is a prerequisite for micropropagation. Although hormonal treatments can induce proliferation in sugar beet rosette explants, they may also result in some side effects. In vitro culture of sugar beet explants and some hormonal treatments make them more prone to hyperhydricity. Effects of media with different concentrations of 6-benzylaminopurine (BAP) and kinetin (Kin) on the proliferation and hyperhydricity of haploid sugar beet explants were investigated. It was observed that 0.2 mg L -1 Kin, with a reasonable amount of proliferation and minimum rate of hyperhydricity, performed better than BAP in different concentrations and combinations. The effect sizes of the treatments on the dependent variables were large. The correlation between proliferation and hyperhydricity of the treated explants was statistically negative and the association was large. However, the hormonal treatments without BAP or with the lowest amount of it produced the highest proliferation rate with the least hyperhydricity. The coefficient of determination was R 2 quadratic = 0.885. The results suggest that, in comparison with BAP, Kin is a potent plant growth regulator for the proliferation of sugar beet haploid explants that causes the least hyperhydricity. Although explants proliferated better in the presence of 0.01 mg L -1 BAP in combination with Kin than under Kin alone, the hyperhydricity of the proliferated explants decreased their suitability for in vitro propagation.

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Section: Resultsmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

A robust method for haploid sugar beet in vitro proliferation and hyperhydricity reduction

Pazuki

Aflaki

Gürel

et al. 2017

Folia Horticulturae

Self Cite

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“…Chromosome set doubling was done using a modified gynogenesis medium previously explained (Pazuki et al, 2018b), in which 2 g L -1 GELRITE was used instead of 2.8 g L -1 for solidification. A 2% solution of colchicine was sterilized using a 22-µm filter.…”

Section: Diploidizationmentioning

confidence: 99%

The effects of proline on in vitro proliferation and propagation of doubled haploid sugar beet (Beta vulgaris)

2018

Turk J Bot

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Doubled haploid induction is one of the available methods normally used for sugar beet breeding. Gynogenic haploid explants of sugar beet induced with 1 or 2 mg L -1 6-benzylaminopurine were treated with 5 g L -1 colchicine, then subcultured on a solidified MS medium plus 0.2 mg L -1 kinetin. Colchicine doubled the chromosome number of 27.7% of the treated haploid explants. With the aim of increasing the number of doubled haploid explants, the effects of five levels of proline (0.0, 0.1, 0.2, 0.3, or 0.4 mM) on the explants' proliferation, propagation, and shoot length were compared. With a large effect size (ES), proline at 0.3 mM induced the highest amount of proliferation, while proline-free medium resulted in the lowest amount of it. The highest propagation rates were observed for the explants treated on media with 0.2 and 0.3 mM proline (very large ES). Proline at 0.3 mM induced the shortest shoots (medium ES). A very strong positive correlation between proliferation and propagation, a moderate negative correlation between proliferation and length, and a strong negative correlation between propagation and length were observed. For the first time our results show beneficial effects of proline on in vitro proliferation and propagation of sugar beet.

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“…The embryo sac is small and embedded in the surrounding tissues, making early embryos difficult to observe and isolate. For plants that are recalcitrant to androgenesis ( e.g ., male-sterile and dioecious plants), gynogenesis is is a worthwhile investigation ( Pazuki et al, 2018 ). In viro gynogenesis has mainly been used on the Cucurbitaceae , and previous studies have revealed that this technique is still imperfect with a low embryogenesis rate ( Metwally et al, 1998 ; Gémes-Juhász et al, 2002 ; Shalaby, 2007 ; Diao et al, 2009 ; Li et al, 2013 ; Plapung, Khumsukdee & Smitamana, 2014 ; Tantasawat et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis of ovary culture-induced embryogenesis in cucumber (Cucumis sativusL.)

Deng

Tang

et al. 2021

PeerJ

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Background. Ovary culture is a useful technique used to generate double haploid (DH) cucumber (Cucumis sativus L.) plants. However, cucumber ovary culture have a low rate of embryo induction and plant regeneration. Moreover, the cucumber embryogenesis mechanism remains unclear. In this study, we explored the molecular basis of cucumber embryogenesis in order to establish a foundation for a more efficient ovary culture method. Using transcriptome sequencing, we also investigated the differential expression of genes during the embryogenesis process. Methods. Cytological and morphological observations have divided cucumber ovary culture into three stages: early embryo development (T0), embryo morphogenesis (T1, T2, T3 and T4), and shoot formation (T5). We selected six key time points for transcriptome sequencing and analysis: T0 (the ovules were cultured for 0 d), T1 (the ovules were cultured for 2 d), T2 (the embryos were cultured for 10 d), T3 (the embryos were cultured for 20 d), T4 (the embryos were cultured for 30 d), and T5 (the shoots after 60 d culture). Results. We used cytology and morphology to observe the characteristics of the cucumber’s developmental transformation during embryogenesis and plant regeneration. The differentially expressed genes(DEGs) at developmental transition points were analyzed using transcriptome sequencing. In the early embryogenesis stage, the cells expanded, which was the signal for gametophytes to switch to the sporophyte development pathway. RNA-seq revealed that when compared to the fresh unpollinated ovaries, there were 3,468 up-regulated genes in the embryos, including hormone signal transduction genes, hormone response genes, and stress-induced genes. The reported embryogenesis-related genes BBM, HSP90 and AGL were also actively expressed during this stage. In the embryo morphogenesis stage (from cell division to cotyledon-embryo formation), 480 genes that functioned in protein complex binding, microtubule binding, tetrapyrrole binding, tubulin binding and other microtubule activities were continuously up-regulated during the T1, T2, T3 and T4 time points. This indicated that the cytoskeleton structure was continuously being built and maintained by the action of microtubule-binding proteins and enzyme modification. In the shoot formation stage, 1,383 genes were up-regulated that were mainly enriched in phenylpropanoid biosynthesis, plant hormone signal transduction, phenylalanine metabolism, and starch and sucrose metabolism. These up-regualted genes included six transcription factors that contained a B3 domain, nine genes in the AP2/ERF family, and two genes encoding WUS homologous domain proteins. Conclusions. Evaluation of molecular gynogenesis events may contribute to a better understanding of the molecular mechanism of cucumber ovarian culture.

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Production of doubled haploids in sugar beet (Beta vulgaris): an efficient method by a multivariate experiment

Cited by 21 publications

References 37 publications

A robust method for haploid sugar beet in vitro proliferation and hyperhydricity reduction

A robust method for haploid sugar beet in vitro proliferation and hyperhydricity reduction

The effects of proline on in vitro proliferation and propagation of doubled haploid sugar beet (Beta vulgaris)

Transcriptome analysis of ovary culture-induced embryogenesis in cucumber (Cucumis sativusL.)

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