Production of Double-stranded DNA Ministrings

Wong, Stephanie E.; Lam, Peggy; Nafissi, Nafiseh; Denniss, Steven G; Slavcev, Roderick A.

doi:10.3791/53177-v

Cited by 2 publications

(1 citation statement)

References 3 publications

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“…This supercoiling will help to prevent insertional mutagenesis. Moreover, several pieces of evidence have shown that there are fewer chances of insertional mutagenesis if the DNA is minicircle or ministering [97][98][99][100]. This is because, in these forms, there is the removal of the major DNA of the bacteria (unmethylated CpG repeats playing the role of PAMPs) [101].…”

Section: Dna Vaccinesmentioning

confidence: 99%

Vaccine development for bacterial pathogens: Advances, challenges and prospects

Elibe

Sharndama

Anyaegbunam

et al. 2023

Tropical Med Int Health

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The advent and use of antimicrobials have played a key role in treating potentially life-threatening infectious diseases, improving health, and saving the lives of millions of people worldwide. However, the emergence of multidrug resistant (MDR) pathogens has been a significant health challenge that has compromised the ability to prevent and treat a wide range of infectious diseases that were once treatable. Vaccines offer potential as a promising alternative to fight against antimicrobial resistance (AMR) infectious diseases. Vaccine technologies include reverse vaccinology, structural biology methods, nucleic acid (DNA and mRNA) vaccines, generalised modules for membrane antigens, bioconjugates/glycoconjugates, nanomaterials and several other emerging technological advances that are offering a potential breakthrough in the development of efficient vaccines against pathogens. This review covers the opportunities and advancements in vaccine discovery and development targeting bacterial pathogens. We reflect on the impact of the already-developed vaccines targeting bacterial pathogens and the potential of those currently under different stages of preclinical and clinical trials. More importantly, we critically and comprehensively analyse the challenges while highlighting the key indices for future vaccine prospects. Finally, the issues and concerns of AMR for low-income countries (sub-Saharan Africa) and the challenges with vaccine integration, discovery and development in this region are critically evaluated. K E Y W O R D Santimicrobial resistant bacteria, bacterial vaccines, emerging vaccine technologies, infectious diseases, lowand middle-income countries, vaccines

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