Production of different molecular weight glycosaminoglycans with microbial cell factories

Wang, Daoan; Hu, Litao; Xu, Ruirui; Zhang, Weijiao; Xiong, Haibo; Wang, Yang; Du, Guocheng; Kang, Zhen

doi:10.1016/j.enzmictec.2023.110324

Cited by 3 publications

(2 citation statements)

References 160 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, most of the NF-DAEs improved the thermal stability of the enzyme, and similar results have been reported in previous studies [ 33 , 37 ]. This result may be related to the structure of the hybrid nanoflower, where the interaction between the organic and inorganic components provides a better secondary structure [ 38 ] that protects the immobilized enzyme at higher temperatures.…”

Section: Resultsmentioning

confidence: 99%

The Formation of D-Allulose 3-Epimerase Hybrid Nanoflowers and Co-Immobilization on Resins for Improved Enzyme Activity, Stability, and Processability

Ding,

Liu,

Huang

et al. 2024

IJMS

View full text Add to dashboard Cite

As a low-calorie sugar, D-allulose is produced from D-fructose catalyzed by D-allulose 3-epimerase (DAE). Here, to improve the catalytic activity, stability, and processability of DAE, we reported a novel method by forming organic–inorganic hybrid nanoflowers (NF-DAEs) and co-immobilizing them on resins to form composites (Re-NF-DAEs). NF-DAEs were prepared by combining DAE with metal ions (Co2+, Cu2+, Zn2+, Ca2+, Ni2+, Fe2+, and Fe3+) in PBS buffer, and were analyzed by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy, and X-ray diffraction. All of the NF-DAEs showed higher catalytic activities than free DAE, and the NF-DAE with Ni2+ (NF-DAE-Ni) reached the highest relative activity of 218%. The NF-DAEs improved the thermal stability of DAE, and the longest half-life reached 228 min for NF-DAE-Co compared with 105 min for the free DAE at 55 °C. To further improve the recycling performance of the NF-DAEs in practical applications, we combined resins and NF-DAEs to form Re-NF-DAEs. Resins and NF-DAEs co-effected the performance of the composites, and ReA (LXTE-606 neutral hydrophobic epoxy-based polypropylene macroreticular resins)-based composites (ReA-NF-DAEs) exhibited outstanding relative activities, thermal stabilities, storage stabilities, and processabilities. The ReA-NF-DAEs were able to be reused to catalyze the conversion from D-fructose to D-allulose, and kept more than 60% of their activities after eight cycles.

show abstract

Section: Resultsmentioning

confidence: 99%

The Formation of D-Allulose 3-Epimerase Hybrid Nanoflowers and Co-Immobilization on Resins for Improved Enzyme Activity, Stability, and Processability

Ding,

Liu,

Huang

et al. 2024

IJMS

View full text Add to dashboard Cite

show abstract

“…93 Most glycosaminoglycans play important roles in various physiological and pathological processes such as cell growth, differentiation, and neurodegeneration. 94 Given the central biological functions of GAGs, there is a strong demand for methods capable of reading the GAG oligosaccharide sequences, some studies have been reported on nanopore detection as follows.…”

Section: Applications Of Nanopore Saccharide Detectionmentioning

confidence: 99%

Recent advances in nanopore-based analysis for carbohydrates and glycoconjugates

Zhao,

Su,

Zhang

et al. 2024

Anal. Methods

View full text Add to dashboard Cite

show abstract

Multi-step semi-synthesis, structural characterization and growth factor interaction study of regiochemically sulfated diabolican polysaccharides

Esposito,

Sinquin,

Colliec-Jouault

et al. 2024

International Journal of Biological Macromolecules

View full text Add to dashboard Cite

Production of different molecular weight glycosaminoglycans with microbial cell factories

Cited by 3 publications

References 160 publications

The Formation of D-Allulose 3-Epimerase Hybrid Nanoflowers and Co-Immobilization on Resins for Improved Enzyme Activity, Stability, and Processability

The Formation of D-Allulose 3-Epimerase Hybrid Nanoflowers and Co-Immobilization on Resins for Improved Enzyme Activity, Stability, and Processability

Recent advances in nanopore-based analysis for carbohydrates and glycoconjugates

Multi-step semi-synthesis, structural characterization and growth factor interaction study of regiochemically sulfated diabolican polysaccharides

Contact Info

Product

Resources

About