Production of dengue virus-like particles serotype-3 in silkworm larvae and their ability to elicit a humoral immune response in mice

Utomo, Doddy Irawan Setyo; Pambudi, Sabar; Sjatha, Fithriyah; Kato, Tatsuya; Park, Enoch Y.

doi:10.1186/s13568-020-01087-3

Cited by 10 publications

(19 citation statements)

References 41 publications

(46 reference statements)

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“…The dengue virus-like particles (DENV-LPs) serotypes 1-4 were prepared according to a previously reported protocol [38]. Briefly, the DENV-LPs were expressed in Bm5 cells and purified using affinity chromatography.…”

Section: Preparation Of Dengue Virus-like Particlesmentioning

confidence: 99%

Design and Analysis of a Single System of Impedimetric Biosensors for the Detection of Mosquito-Borne Viruses

et al. 2021

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The treatment for mosquito-borne viral diseases such as dengue virus (DENV), zika virus (ZIKV), and chikungunya virus (CHIKV) has become difficult due to delayed diagnosis processes. In addition, sharing the same transmission media and similar symptoms at the early stage of infection of these diseases has become more critical for early diagnosis. To overcome this, a common platform that can identify the virus with high sensitivity and selectivity, even for the different serotypes, is in high demand. In this study, we have attempted an electrochemical impedimetric method to detect the ZIKV, DENV, and CHIKV using their corresponding antibody-conjugated sensor electrodes. The significance of this method is emphasized on the fabrication of a common matrix of gold–polyaniline and sulfur, nitrogen-doped graphene quantum dot nanocomposites (Au-PAni-N,S-GQDs), which have a strong impedimetric response based only on the conjugated antibody, resulting in minimum cross-reactivity for the detection of various mosquito-borne viruses, separately. As a result, four serotypes of DENV and ZIKV, and CHIKV have been detected successfully with an LOD of femtogram mL–1.

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Section: Preparation Of Dengue Virus-like Particlesmentioning

confidence: 99%

Design and Analysis of a Single System of Impedimetric Biosensors for the Detection of Mosquito-Borne Viruses

et al. 2021

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“…Rabbit antisera were generated from the fusion protein which was stable [26]. A domain of the carboxy terminus of the E gene is responsible for mediating E-prM interactions in dengue virus, while a peptide in the ectodomain of the E gene mediates cell activity in dengue virus [27].…”

Section: Membrane Associated Protein (M)mentioning

confidence: 99%

Heparan Sulfate: Landing Site for the Dengue Viral Strains to Gain Entry to the Host Cells

Papulzai¹,

John²,

Malla³

2021

Asian J Pharm Clin Res

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Humans usually contract dengue by being bitten by arthropods, and more than 3.6 billion people are at risk per year. Although studies are conducted to screen and trace out the possible pathophysiology of the virus, an adequate receptor-based study has not been completed. Understanding how the dengue virus (DV) engraves its landing sites requires identification of such cellular receptors. In many model studies, heparan sulfate (HS) has been reported to act as a DV receptor under various conditions. However, the physiological relevance of these findings remains uncertain. Therefore, it is still unclear whether HS is used by viral strains or not, and if at all used by clinical or non-cell culture-adapted strains of DV. The present review aims to identify relevant experimental evidences that confirm the possible interaction between envelope protein and HS chains. We collected data from a series of studies to conclude the interactive role.

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“…The heparin-binding assay by ELISA was performed with modi cations as previously described (Utomo et al 2019;Utomo et al 2020). Six ng/ml diluted biotin-labeled heparin (Sigma-Aldrich Japan) and 1.8 ng of heparin were immobilized into blockless avidin plate (Sumitomo Bakelite, Tokyo, Japan) wells and washed three times with PBS.…”

Section: Western Blot Analysismentioning

confidence: 99%

“…Previously, we have successfully expressed DENV-LPs serotype 2 (DENV-LP/2CprME, /2E), and DENV-LPs serotype 3 (DENV-LP/3prME, /3CprME) by removing capsid-anchor in the C region (Utomo et al 2019;Utomo et al 2020). In this study, we prepared DENV-LPs consisting full-length of capsid-premembraneenvelope (1CprME and 4CprME) and premembrane-envelope (1prME and 4prME) polypeptides from serotypes 1 and 4, which were expressed using Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid in silkworms.…”

Section: Introductionmentioning

confidence: 99%

Humoral Immune Response in a Mouse Model Induced With Dengue Virus-like Particles Serotypes 1 and 4 Produced in Silkworm Larvae

Irawan

Pambudi

Park

2021

Preprint

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Dengue is an arboviral disease, which threatens almost half the global population, and has emerged as the most significant of current global public health challenges. In this study, we prepared dengue virus-like particles (DENV-LPs) consisting of Capsid-Premembrane-Envelope (CprM/E) and Premembrane-Envelope (prM/E) polypeptides from serotype 1 and 4, which were expressed in the silkworms using Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid. 1CprME, 1prME, 4CprME, and 4prME expressed proteins in hemolymph and molecular weight of the purified proteins were 55 kDa, respectively. The purified polypeptides formed spherical Dengue virus-like particles (DENV-LPs) with approximately 30–55 nm in diameter. The immunoelectron microscopy (IEM) images revealed antigens to the surface of a lipid bilayer of DENV-LPs. The heparin-binding assay shows a positive relationship between absorbance and the quantity of E protein domain III (EDIII), which was supported by the isothermal titration calorimetry assay, showing a moderate binding affinity between heparin and DENV-LP. The high correlation between patient sera and DENV-LP reactivities revealed that these DENV-LPs shared similar epitopes with the natural dengue virus. IgG elicitation studies in mice have demonstrated that DENV-LP/CPrMEs elicits a stronger immune response than DENV-LP/prMEs, which lends credence to this claim.

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Production of dengue virus-like particles serotype-3 in silkworm larvae and their ability to elicit a humoral immune response in mice

Cited by 10 publications

References 41 publications

Design and Analysis of a Single System of Impedimetric Biosensors for the Detection of Mosquito-Borne Viruses

Design and Analysis of a Single System of Impedimetric Biosensors for the Detection of Mosquito-Borne Viruses

Heparan Sulfate: Landing Site for the Dengue Viral Strains to Gain Entry to the Host Cells

Humoral Immune Response in a Mouse Model Induced With Dengue Virus-like Particles Serotypes 1 and 4 Produced in Silkworm Larvae

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