Production of d-lactic acid in a continuous membrane integrated fermentation reactor by genetically modified Saccharomyces cerevisiae: Enhancement in d-lactic acid carbon yield

Mimitsuka, Takashi; Sawai, Kenji; Kobayashi, Kōji; Tsukada, Takeshi; Takeuchi, Norihiro; Yamada, Katsushige; Ogino, Hiroyasu; Yonehara, Tetsu

doi:10.1016/j.jbiosc.2014.06.002

Cited by 33 publications

(17 citation statements)

References 28 publications

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“…In addition to the operation mode of fermentation, various previous investigations in the literature also used approaches from different aspects to intensify the LA production, reducing its production cost, e.g., utilizing economical feedstock sources [50,51,52], selecting highly-productive strains of microorganisms [53], and optimizing fermentation conditions [31,54]. Moreover, the lactate in the permeate can be treated further by other membrane processes, e.g., bipolar electrodialysis can convert it into LA, while regenerated alkaline can be reused for the neutralization of the fermentation broth [55,56,57].…”

Section: Discussionmentioning

confidence: 99%

Anaerobic Membrane Bioreactor for Continuous Lactic Acid Fermentation

2017

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Membrane bioreactor systems can enhance anaerobic lactic acid fermentation by reducing product inhibition, thus increasing productivity. In batch fermentations, the bioconversion of glucose is strongly inhibited in the presence of more than 100 g·L−1 lactic acid and is only possible when the product is simultaneously removed, which can be achieved by ceramic membrane filtration. The crossflow velocity is a more important determinant of flux than the transmembrane pressure. Therefore, to stabilize the performance of the membrane bioreactor system during continuous fermentation, the crossflow velocity was controlled by varying the biomass concentration, which was monitored in real-time using an optical sensor. Continuous fermentation under these conditions, thus, achieved a stable productivity of ~8 g·L−1·h−1 and the concentration of lactic acid was maintained at ~40 g·L−1 at a dilution rate of 0.2 h−1. No residual sugar was detected in the steady state with a feed concentration of 50 g·L−1.

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Section: Discussionmentioning

confidence: 99%

Anaerobic Membrane Bioreactor for Continuous Lactic Acid Fermentation

2017

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“…The OD 600 and concentrations of glucose, ethanol, and glycerol in each culture broth were measured as described previously (Yamada et al, ). The yeast dry cell weight (DCW) and carbon weight were calculated using the conversion coefficients 0.33 g‐DCW/L/OD 600 and 0.47 g‐carbon/g‐DCW (Mimitsuka et al, ). The d ‐ and l ‐lactic acid concentrations were determined using the d ‐ and l ‐lactic acid assay kit (Megazyme, Wicklow, Ireland) and the Multiskan GO microplate reader (Thermo Scientific, Rochester, NY).…”

Section: Methodsmentioning

confidence: 99%

“…However, heterologous expression of d ‐LDH and d ‐lactic acid production was limited. Some research groups have heterologously expressed d ‐LDH from Leuconostoc mesenteroides (Baek et al, ; Ishida et al, ) or Limulus polyphemus (Mimitsuka et al, ), and optically pure d ‐lactic acid production was achieved. Baek et al () reported that the quadruple deletion of PDC1, ADH1, GPD1 (glycerol‐3‐phosphate dehydrogenase), and GPD2 in d ‐LDH expressing S. cerevisiae resulted in higher d ‐lactate yield.…”

Section: Introductionmentioning

confidence: 99%

Enhanced d‐lactic acid production by recombinant Saccharomyces cerevisiae following optimization of the global metabolic pathway

Yamada

Wakita

Mitsui

et al. 2017

Biotech & Bioengineering

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Utilization of renewable feedstocks for the production of bio-based chemicals such as d-lactic acid by engineering metabolic pathways in the yeast Saccharomyces cerevisiae has recently become an attractive option. In this study, to realize efficient d-lactic acid production by S. cerevisiae, the expression of 12 glycolysis-related genes and the Leuconostoc mesenteroides d-LDH gene was optimized using a previously developed global metabolic engineering strategy, and repeated batch fermentation was carried out using the resultant strain YPH499/dPdA3-34/DLDH/1-18. Stable d-lactic acid production through 10 repeated batch fermentations was achieved using YPH499/dPdA3-34/DLDH/1-18. The average d-lactic acid production, productivity, and yield with 10 repeated batch fermentations were 60.3 g/L, 2.80 g/L/h, and 0.646, respectively. The present study is the first report of the application of a global metabolic engineering strategy for bio-based chemical production, and it shows the potential for efficient production of such chemicals by global metabolic engineering of the yeast S. cerevisiae. Biotechnol. Bioeng. 2017;114: 2075-2084. © 2017 Wiley Periodicals, Inc.

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“…Although the quantity of lactic acid generated varies widely depending on the strain of yeast used (Branduardi et al 2006), these earlier studies generated less than 0.5 g lactic acid/l (Pacheco et al 2012). An almost doubling of lactate yield to 0.75 g/g glucose under continuous culture conditions led to speculation that this operational mode may be advantageous for lactic acid production by S. cerevisiae because such a process maintains a very low glucose concentration and thus reduces the Crabtree effect found in this yeast (Mimitsuda et al 2014).…”

Section: Membrane Transport Associated With Lactate Formationmentioning

confidence: 99%

Microbial production of lactic acid

Eiteman

Ramalingam

2015

Biotechnol Lett

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Lactic acid is an important commodity chemical having a wide range of applications. Microbial production effectively competes with chemical synthesis methods because biochemical synthesis permits the generation of either one of the two enantiomers with high optical purity at high yield and titer, a result which is particularly beneficial for the production of poly(lactic acid) polymers having specific properties. The commercial viability of microbial lactic acid production relies on utilization of inexpensive carbon substrates derived from agricultural or waste resources. Therefore, optimal lactic acid formation requires an understanding and engineering of both the competing pathways involved in carbohydrate metabolism, as well as pathways leading to potential by-products which both affect product yield. Recent research leverages those biochemical pathways, while researchers also continue to seek strains with improved tolerance and ability to perform under desirable industrial conditions, for example, of pH and temperature.

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Production of d-lactic acid in a continuous membrane integrated fermentation reactor by genetically modified Saccharomyces cerevisiae: Enhancement in d-lactic acid carbon yield

Cited by 33 publications

References 28 publications

Anaerobic Membrane Bioreactor for Continuous Lactic Acid Fermentation

Anaerobic Membrane Bioreactor for Continuous Lactic Acid Fermentation

Enhanced d‐lactic acid production by recombinant Saccharomyces cerevisiae following optimization of the global metabolic pathway

Microbial production of lactic acid

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