“…It has been previously shown that pathogenicity of E. herbicola on Gypsophila plants is correlated with cytokinin production (17). Accordingly, PCR was employed in order to test the prevalence and location of pre-etz and etz among pathogenic strains.…”
Section: Dna Sequence Analysismentioning
confidence: 99%
“…gypsophilae induces gall formation on Gypsophila plants (9), whereas other strains of E. herbicola generate galls on table beet also (7). This pathogen has been shown to possess genes specifying indole-3-acetic acid (IAA) production (8) and a locus conferring cytokinin biosynthesis (17). These genes, encoding the phytohormones, are clustered on a native plasmid designated pPATH which is present only in pathogenic strains (17,18).…”
mentioning
confidence: 99%
“…This pathogen has been shown to possess genes specifying indole-3-acetic acid (IAA) production (8) and a locus conferring cytokinin biosynthesis (17). These genes, encoding the phytohormones, are clustered on a native plasmid designated pPATH which is present only in pathogenic strains (17,18). Insertional inactivation of the IAA biosynthesis genes on pPATH resulted in smaller galls but did not eliminate their formation (8).…”
mentioning
confidence: 99%
“…gypsophilae secretes zeatin, zeatin riboside, iso-pentenyladenine, and two unidentified immunoreactive zeatin-type compounds (17). A 3.7-kb SmaI DNA fragment was subcloned from a cosmid clone derived from pPATH.…”
A locus conferring cytokinin production was previously isolated from the gall-forming bacterium Erwinia herbicola pv. gypsophilae. This locus resided in a cluster with the genes specifying indole-3-acetic acid production on the pathogenicity-associated plasmid pPATH ( Complementation of this marker exchange mutant with the intact etz gene on a multicopy plasmid resulted in overproduction of cytokinins and larger plant galls from which small shoots emerged. Insertional mutation in pre-etz resulted in a sharp decrease in both the level of the etz-specific transcript and cytokinin production. A frameshift mutation in pre-etz caused a similar reduction in the cytokinin level. A marker exchange mutation in pre-etz caused a reduction of symptoms but to a lower degree than the etz mutation. In the former mutant, cytokinin production and pathogenicity could not be restored by complementation. Furthermore, attempts to complement the etz marker exchange mutant with a plasmid containing an intact etz gene and a frameshift mutation in the pre-etz gene were unsuccessful. These results suggest that the mutations in pre-etz were trans dominant.
“…It has been previously shown that pathogenicity of E. herbicola on Gypsophila plants is correlated with cytokinin production (17). Accordingly, PCR was employed in order to test the prevalence and location of pre-etz and etz among pathogenic strains.…”
Section: Dna Sequence Analysismentioning
confidence: 99%
“…gypsophilae induces gall formation on Gypsophila plants (9), whereas other strains of E. herbicola generate galls on table beet also (7). This pathogen has been shown to possess genes specifying indole-3-acetic acid (IAA) production (8) and a locus conferring cytokinin biosynthesis (17). These genes, encoding the phytohormones, are clustered on a native plasmid designated pPATH which is present only in pathogenic strains (17,18).…”
mentioning
confidence: 99%
“…This pathogen has been shown to possess genes specifying indole-3-acetic acid (IAA) production (8) and a locus conferring cytokinin biosynthesis (17). These genes, encoding the phytohormones, are clustered on a native plasmid designated pPATH which is present only in pathogenic strains (17,18). Insertional inactivation of the IAA biosynthesis genes on pPATH resulted in smaller galls but did not eliminate their formation (8).…”
mentioning
confidence: 99%
“…gypsophilae secretes zeatin, zeatin riboside, iso-pentenyladenine, and two unidentified immunoreactive zeatin-type compounds (17). A 3.7-kb SmaI DNA fragment was subcloned from a cosmid clone derived from pPATH.…”
A locus conferring cytokinin production was previously isolated from the gall-forming bacterium Erwinia herbicola pv. gypsophilae. This locus resided in a cluster with the genes specifying indole-3-acetic acid production on the pathogenicity-associated plasmid pPATH ( Complementation of this marker exchange mutant with the intact etz gene on a multicopy plasmid resulted in overproduction of cytokinins and larger plant galls from which small shoots emerged. Insertional mutation in pre-etz resulted in a sharp decrease in both the level of the etz-specific transcript and cytokinin production. A frameshift mutation in pre-etz caused a similar reduction in the cytokinin level. A marker exchange mutation in pre-etz caused a reduction of symptoms but to a lower degree than the etz mutation. In the former mutant, cytokinin production and pathogenicity could not be restored by complementation. Furthermore, attempts to complement the etz marker exchange mutant with a plasmid containing an intact etz gene and a frameshift mutation in the pre-etz gene were unsuccessful. These results suggest that the mutations in pre-etz were trans dominant.
“…In another study, Taller and Wong (1989) determined cytokinins as equivalent to 0.75 μg of kinetin per litre in Azotobacter vinelandii culture medium while Karnwal and Kaushik (2011) reported 5.5 and 2.9 pmol/ml of cytokinins per litre for P. fluorescens and Pseudomonas aeruginosa, respectively. However, Lichter et al (1995) analyzed different forms of cytokinins that is, zeatin, zeatin riboside, isopentenyladenin and two immunoreactive zeatin type compounds in the culture supernatant of pathogenic strain Erwinia herbicola and quantified zeatin in range of 200 to 300 ng/ml and zeatin riboside 13 to 107 ng/ml. So the selected Pseudomonas isolates were found to be quite efficient for the production of cytokinins like substances.…”
Information on microbial production of cytokinins and their effect on plant growth are limited. Therefore, the objective of the present study was to investigate cytokinins production by Pseudomonas sp., a major component of rhizobacteria with multiform and diverse activities, which enhance plant growth by synthesizing various secondary metabolites. In the present investigations, thirty Pseudomonas isolates were isolated from the rhizosphere of Pyrus and Malus and were screened for cytokinins production (50 to 210 μg/ml). Four strains viz PN-4-SAN, PN-10-SAN, AN-2-NAG and AN-4-NAG were selected on the basis of their higher cytokinins production. The maximum cytokinins production was observed at 72h incubation period in nutrient broth at pH 7.0 under shaken condition at 28°C. Cytokinins were extracted, purified and evaluated by thin layer chromatography and specific bioassay method.
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