Production of Cloned Pigs by Nuclear Transfer of Preadipocytes Established from Adult Mature Adipocytes

Tomii, Ryo; Kurome, Mayuko; Ochiai, Takashi; Wako, Naohiro; Ueda, Hideto; Hirakawa, Kazumasa; Kano, Koichiro; Nagashima, Hiroshi

doi:10.1089/clo.2005.7.279

Cited by 36 publications

(49 citation statements)

References 43 publications

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“…Compared to adult-derived donor cells, FFs resulted in a lower blastocyst rate. Our results were in agreement with the observation of Tomii et al (2005), in which the blastocyst rate of AP-derived embryos was slightly higher than that of FF-derived embryos. However, it was reported by different laboratories that FFs were superior to adult-derived donor cells.…”

Section: Discussionsupporting

confidence: 92%

“…Most previous publications failed to investigate the in vivo development or data collected were insufficient for statistical calculations (Faast et al, 2006;Tomii et al, 2005;Uhm et al, 2000;Yin et al, 2002). Our results confirmed that the capacity of nucleus to be reprogrammed cannot be evaluated exclusively by in vitro development; in contrast, in vivo development may be a more important characteristic.…”

Section: Discussionsupporting

confidence: 78%

“…The difference may be due mainly to different types of adult-derived donor cells used in our study and those papers. APs and BMs in the present study were two adult-derived donor cells that have been demonstrated to be multipotent by different reports (Faast et al, 2006;Tomii et al, 2005).…”

Section: Discussionmentioning

confidence: 66%

“…APs were obtained as reported by Tomii et al (2005). Fat tissue samples were collected from the anterior abdominal wall of pigs by surgery, washed with PBS three times, and cut into small pieces (approximate 2-mm 3 cubes) with fine scissors and scalpels.…”

Section: Primary Cell Lines Establishment and Donor Cells Preparationmentioning

confidence: 99%

“…In recent studies, to correct or relieve the incomplete epigenetic reprogramming of cloned embryos, different cell types were used as the nuclear donor for pig SCNT, such as fetal fibroblasts (FFs; Onishi et al, 2000), preadipocytes (Tomii et al, 2005), adult mesenchymal stem cells (MSCs; Faast et al, 2006), recloned pig somatic cells (Cho et al, 2007), and induced pluripotent stem cells (iPSCs; Fan et al, 2013).…”

mentioning

confidence: 99%

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Factors Determining the Efficiency of Porcine Somatic Cell Nuclear Transfer: Data Analysis with Over 200,000 Reconstructed Embryos

Liu

Dou

Xiang

et al. 2015

Cellular Reprogramming

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Data analysis in somatic cell nuclear transfer (SCNT) research is usually limited to several hundreds or thousands of reconstructed embryos. Here, we report mass results obtained with an established and consistent porcine SCNT system (handmade cloning [HMC]). During the experimental period, 228,230 reconstructed embryos and 82,969 blastocysts were produced. After being transferred into 656 recipients, 1070 piglets were obtained. First, the effects of different types of donor cells, including fetal fibroblasts (FFs), adult fibroblasts (AFs), adult preadipocytes (APs), and adult blood mesenchymal (BM) cells, were investigated on the further in vitro and in vivo development. Compared to adult donor cells (AFs, APs, BM cells, respectively), FF cells resulted in a lower blastocyst/reconstructed embryo rate (30.38% vs. 37.94%, 34.65%, and 34.87%, respectively), but a higher overall efficiency on the number of piglets born alive per total blastocysts transferred (1.50% vs. 0.86%, 1.03%, and 0.91%, respectively) and a lower rate of developmental abnormalities (10.87% vs. 56.57%, 24.39%, and 51.85%, respectively). Second, recloning was performed with cloned adult fibroblasts (CAFs) and cloned fetal fibroblasts (CFFs). When CAFs were used as the nuclear donor, fewer developmental abnormalities and higher overall efficiency were observed compared to AFs (56.57% vs. 28.13% and 0.86% vs.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 66%

Section: Primary Cell Lines Establishment and Donor Cells Preparationmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Factors Determining the Efficiency of Porcine Somatic Cell Nuclear Transfer: Data Analysis with Over 200,000 Reconstructed Embryos

Liu

Dou

Xiang

et al. 2015

Cellular Reprogramming

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Differentiation of 3T3‐L1 preadipocytes is inhibited under a modified ceiling culture

Song

Cheng

Yang

et al. 2015

Cell Biology International

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Ceiling culture is an inverted and closed cell culture system which represents a novel method for exploring adipocyte characteristics and function. Although the role of ceiling culture in mature adipocyte dedifferentiation has been extensively studied, its potential effects on preadipocyte differentiation remain unclear. In this study, we established a simplified dish ceiling culture method for 3T3-L1 preadipocytes and showed that our novel ceiling culture method could reproduce the function of the traditional flask ceiling culture. Then, we investigated the effects of ceiling culture on 3T3-L1 preadipocyte differentiation by Oil red O staining and RT-qPCR. The results showed that ceiling culture significantly impaired triglyceride accumulation and adipogenic marker genes expression in 3T3-L1 preadipocytes. These findings suggest that ceiling culture inhibited 3T3-L1 preadipocyte differentiation while inducing mature adipocytes dedifferentiation. Taken together, our data facilitate the understanding of the property of ceiling culture and promote the study of revealing the underlying mechanisms of mature adipocytes dedifferenatiation.

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Establishment and characteristics of porcine preadipocyte cell lines derived from mature adipocytes

Nobusue

Kano

2009

J of Cellular Biochemistry

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Development of established preadipocyte cell lines, such as 3T3-L1 and 3T3-F442A, greatly facilitated the study of molecular mechanisms of adipocyte differentiation under defined conditions. Most of these cell lines are derived from mouse embryos, and preadipocyte cell lines of other species have not yet been maintained in culture long enough to study differentiation under a variety of conditions. This is the first report on the establishment of porcine preadipocyte cell lines derived from mature adipocytes by a simple method, known as ceiling culture, for culturing mature adipocytes in vitro. This cell line can proliferate extensively until the cells become confluent and fully differentiated into mature adipocytes, depending on adipogenic induction. No changes in their differentiation pattern are observed during their propagation, and they have been successfully carried and differentiated for at least 37 passages. This cell line maintains a normal phenotype without transforming spontaneously, even after long-term maintenance in culture. This achievement may lead to easy establishment of porcine preadipocyte cell lines and novel model systems for studying the mechanisms of adipocyte differentiation and metabolism as a substitute for human preadipocytes.

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Production of Cloned Pigs by Nuclear Transfer of Preadipocytes Established from Adult Mature Adipocytes

Cited by 36 publications

References 43 publications

Factors Determining the Efficiency of Porcine Somatic Cell Nuclear Transfer: Data Analysis with Over 200,000 Reconstructed Embryos

Factors Determining the Efficiency of Porcine Somatic Cell Nuclear Transfer: Data Analysis with Over 200,000 Reconstructed Embryos

Differentiation of 3T3‐L1 preadipocytes is inhibited under a modified ceiling culture

Establishment and characteristics of porcine preadipocyte cell lines derived from mature adipocytes

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