Production of Cloned Amago Salmon &lt;i&gt;Oncorhynchus rhodurus&lt;/i&gt;

Kobayashi, Toru; Ide, A.; Hiasa, Takayuki; Fushiki, Shozo; Ueno, Koichi

doi:10.2331/fishsci.60.275

Cited by 47 publications

(21 citation statements)

References 15 publications

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“…The HRs of the heterozygous clonal female and meiotic gynogenetic female groups were 34.3 ± 8.5 and 28.9 ± 6.6%, respectively, both higher than the value in rainbow trout. Previous studies have reported that the survival rate of DHs was quite low: only 4–20% in zebrafish, Danio rerio (Hörstgen‐Schwark ); only 4 of 10 hatched fry survived to adulthood in medaka, Oryzias latipes (Naruse et al ); in the rosy bitterling, Rhodeus ocellatus , survival of DHs was 5.5% between hatching and 30 d posthatching (Kawamura ); and in a study of DH amago salmon, Oncorhynchus masou ishikawae , only 6 out of 98 (6.1%) first‐feeding DH survived until the spawning season 2 yr later (Kobayashi et al ). After hatching, there are two stages of massive mortality for DHs in Japanese flounder: the first‐feeding stage, with an unopened oral membrane, and the metamorphosis stage, which directly results in severe deformation, especially of the cranium and vertebral column.…”

Section: Discussionmentioning

confidence: 99%

Mass Production of Doubled Haploids in Japanese Flounder, Paralichthys olivaceus

Zhang

Wang

Sun

et al. 2017

J World Aquaculture Soc

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Doubled haploid (DH) individuals have many applications in fish genetic and genomic research, as well as in fish breeding programs. Mass production of DHs is difficult because of an extremely low induction rate. Here, we report the mass production of mitotic gynogenetic DHs in Japanese flounder, Paralichthys olivaceus, an economically important marine‐culture fish species. Four groups of mitotic gynogenetic DHs were induced from four females of different genetic backgrounds. The heterozygous clonal female group had the highest hatching rate, lowest abnormality rate, and highest homozygosity rate. Early growth comparisons showed that the body weight and total length of the heterozygous clonal female group were the largest among the three DH groups (P < 0.05). At 6 mo postinduction, an estimated 10,766 DHs survived, and a notable percentage of male DHs (3.34–23.33%) were detected. This successful mass production of DHs lays the foundation for extensive use of DHs in Japanese flounder breeding and research.

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Section: Discussionmentioning

confidence: 99%

Mass Production of Doubled Haploids in Japanese Flounder, Paralichthys olivaceus

Zhang

Wang

Sun

et al. 2017

J World Aquaculture Soc

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“…1991), common carp Cyprinus carpio (Komen et al. 1991), amago salmon Oncorhynchus rhodurus (Kobayashi et al. 1994) and Japanese flounder Paralichthys olivaceus (Yamamoto 1995,1999), offspring were produced from mitotic‐G2N broodstock.…”

Section: Discussionmentioning

confidence: 99%

Viability, growth and external morphology of meiotic- and mitotic-gynogenetic diploids in red sea bream, Pagrus major

Kato

Murata

Yamamoto

et al. 2008

Journal of Applied Ichthyology

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Two types of gynogenetic diploids were arti®cially induced in the red sea bream (Pagrus major Temminck et Schlegel), either by suppressing the ®rst cell cleavage (mitotic-G2N) or by retaining the second polar body (meiotic-G2N). The eggs of red sea bream were inseminated with UV-irradiated (3000 erg mm )2 ) sperm of Japanese parrot ®sh (Oplegnathus fasciatus Temminck et Schlegel), and hydrostatic pressure shock of 700 kg cm )2 for 5.5 min at 46 min after insemination (mitotic-G2N) and cold shock of 1°C for 30 min at 3 min after insemination (meiotic-G2N) were applied to the eggs, sequentially. The total hatching rate and hatching rate of normal larvae of the normal diploid, meiotic-G2N and mitotic-G2N were 86.5 and 94.9%, 38.1 and 45.8%, and 12.8 and 35.0%, respectively. The induction of mitotic-G2N was con®rmed by isozyme marker analysis. The standard deviations, variances and coecients of variation of the body weight, standard length and body depth in 91-day-old juveniles were always large in mitotic-G2N, small in normal-2N and intermediate in meiotic-G2N. The variances in the number of pectoral ®n rays and caudal ®n rays of mitotic-G2N were signi®cantly higher than those of normal-2N. The incidences of deformities were highest in the mitotic-G2N group. The survival rates and growth performance of the meiotic-and mitotic-G2N were signi®cantly lower than those of normal-2N. Both G2N survived for 3 years to the adult stage.

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“…However, clonal lines have been generated in a limited number of fish species due to the low survival of DHs and disturbances during their gonadal development and oogenesis (Hou et al, 2016;Hou, Fujimoto, Saito, Yamaha, & Arai, 2015;Kato et al, 2002;Kobayashi, Ide, Hiasa, Fushiki, & Ueno, 1994;Komen, Bongers, Richter, Van Muiswinkel, & Huisman, 1991;Naruse, Ijiri, Shima, & Egami, 1985;Sarder, Penman, Myers, & McAndrew, 1999;Yamamoto, 1999;Young, Wheeler, Fields, & Thorgaard, 1996;Zhang et al, 2015). DH females may be sterile or produce poor quality eggs exhibiting decreased ability to be fertilized and impaired competences for the proper development of embryos (Migaud et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Developmental competence of eggs produced by rainbow trout Doubled Haploids (DHs) and generation of the clonal lines

Jagiełło

Dobosz

Zalewski

et al. 2018

Reprod Domestic Animals

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Poor quality eggs produced by the fully homozygous doubled haploids (DHs) may impair generation of clonal lines in fish species. In the present research, gynogenetic development of rainbow trout (Oncorhynchus mykiss) was induced in eggs originated from the DH females. Eggs were activated with the UV-irradiated grayling (Thymallus thymallus) spermatozoa and subjected to the high hydrostatic pressure (HHP) shock to provide diploid clonal individuals. Only two of four DH females produced eggs that were successfully activated by the irradiated spermatozoa and subsequently developed into the gynogenetic embryos. Survival rates of rainbow trout from the clonal lines equalled 21.5% and 19.8% during embryogenesis and decreased after hatching to 18.6% and 14.9%, respectively. Some of the dead rainbow trout clones collected between hatching and swim-up stage were emaciated and exhibited spinal deformities including scoliosis. Provided results confirmed limited developmental competences of eggs produced by rainbow trout DH females. Clonal rainbow trout developing in such eggs exhibited reduced survival and increased frequency of the body abnormalities.

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Production of Cloned Amago Salmon <i>Oncorhynchus rhodurus</i>

Abstract: We induced homozygous clones of amago salmon by gynogenetic chromosome manipulation. Mitotic

Cited by 47 publications

References 15 publications

Mass Production of Doubled Haploids in Japanese Flounder, Paralichthys olivaceus

Mass Production of Doubled Haploids in Japanese Flounder, Paralichthys olivaceus

Viability, growth and external morphology of meiotic- and mitotic-gynogenetic diploids in red sea bream, Pagrus major

Developmental competence of eggs produced by rainbow trout Doubled Haploids (DHs) and generation of the clonal lines

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