Production of clonal plantlets of tropical pine in tissue culture via axillary shoot activation

Baxter, Robert C.; Brown, S. N.; England, N.; Ludlow, C. H. M.; Taylor, Skye L.; Womack, R. W.

doi:10.1139/x89-206

Cited by 18 publications

(11 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The micropropagation of P. caribaea using different media and methods was previously reported (Webb & Santiago 1983;Berlyn et al 1987;Skidmore et al 1988;Baxter et al 1989). These studies showed that benzyladenine is necessary in the induction of adventitious shoots.…”

Section: Introductionmentioning

confidence: 94%

In vitro response of embryos from different provenances of Pinus caribaea var. hondurensis Morelet

Perez-Orozeo

Halos

1993

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

The tissue culture response of embryo explants of four fast-and slow-growing provenances of Pinus caribaea var. hondurensis was investigated. Treatments included adventitious bud induction and development with benzyladenine (BA) at different concentrations and exposure periods, root induction with naphthaleneacetic acid (NAA) and indolebutyric acid (IBA) and callus production with combinations of NAA, 2, 4-dichlorophenoxyacetic acid (2, 4-D), BA and kinetin. Fast-growing provenances produced nodules earlier on the cotyledonary surface, developed shoots earlier from induced buds and produced more shoots per surviving embryo at lower BA concentrations tested compared with slow-growing provenances. On the other hand, calluses of the slow-growing provenance, La Mosquitia, grew faster on media supplemented with cytokinins and auxins compared with those grown on media supplemented with auxin only. This reaction was not observed with fast-growing provenances. It is suggested that cytokinins in fast-growing provenances promoted organized development more efficiently than in slow-growing provenances.

show abstract

Section: Introductionmentioning

confidence: 94%

In vitro response of embryos from different provenances of Pinus caribaea var. hondurensis Morelet

Perez-Orozeo

Halos

1993

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…After culturing for 4 weeks, axillary buds were actively flushed as shown in Fig.l. The flushed shoots were transferred directly into GD medium containing less than 1 btM naphthaleneacetic acid (NAA), for shooting of axillary buds, according to the method of Baxter et al (1989).…”

Section: Collection and Sterilization Of Explantsmentioning

confidence: 99%

Effect of l-Phenylalanine on Rooting of Shoots from Axillary Buds of Adult Larix Leptolepis

Ogita

Kubo

Fushitani

1997

Journal of Forest Research

View full text Add to dashboard Cite

Rooting of shoots derived from axillary buds was examined to establish an efficient shoot culture system of clonal micropropagation in adult tree of Larix leptolepis Gord. (Japanese larch). Nine out of ten shoots induced calli (90%) on their shoot bases, and the two of them formed root primordia with a red pigment (20%) on the calli surface within 5 weeks after culturing on modified Murashige and Skoog (MS) medium supplemented with 1.5 btM of indolebutyric acid (1BA) and 1.5 laM of naphthaleneacetic acid (NAA). However, the primordia did not elongate actively. The addition of 10 mM L-phenylalanine in the MS medium with the auxins resulted in the formation of roots at high fi'equency, about 80%, and they elongated actively. Although callus was formed in all the shoots cultured on the medium with L-phenylalanine, it appeared that the callus development was less as compared to the medi um without L-phenylalanine. Consequently, the rooting might be associated with the suppression of the induced callus.Key words : adult tree, LarLv leptolepis, L-phenylalanine, root formation Establishment of a clonal propagation system by tissue culture is very important to produce the dominant trees, in points of yield, quality, adaptability and resistance. A cutting, which is one of asexual propagation method, has been generally practiced for genetic improvement of species such as C13~ptomeria japonica and Chamaecyparis obtusa (Mikami, 1991). These species can root in vitro (Ishikawa, 1986;Amano, 1989). However, in some other species such as Larix leptolepis, it is difficult to induce roots directly in the cuttings, or in vitro, in case of using shoots from the adult tree. It is reported that the rate of rooting was less than 30% in shoots of trees over 10-year-old (Ishikawa, 1962;Okada, 1967). Thus, the authors attempted to establish a new propagation system by shoot culture for theimprovement of rooting in the adult tree of L. leptolepis.In L leptolepis, bud culture has been achieved in vitro (Itahana, 1993), but the explant is confined to an apical bud in shoots. Tissue culture of axillary buds in shoots from an adult tree is an effective method to propagate the dominant trees.However, it seems that achievement is not notable in L. leptolepis, compared with such species as C. obtusa, Piing brutia, Pinus radiata (Amano, 1989;Abdullah et al., 1987;Horgan and Holland, 1989). This may be lmgely due to heavy contamination which occurs peculiarly in the adult tree of Larix species. The problem has been partly solved by authors who investigated for conditions to effectively sterilize the explants and also for culture conditions to flush more frequently axillary buds in shoots from an adult tree of L. leptolepis of more than 20-year-old (Ogita et al., 1995). Another problem is the fact that the rooting percentage is remarkably poor in shoots originating from axillary buds of adult tree. It has been generally known that root formation in shoot culture of woody plants is mainly controlled by some kind of auxin and its concentration in a su...

show abstract

“…Baxter et al (1989) obtained rooting in nonsterile conditions in a peat:perlite medium with daily misting under a sealed propagator.…”

Section: Rooting Of Adventitious Shootsmentioning

confidence: 99%

“…Rooting in conifers generally requires exogenous application of auxins (Mohammed & Vidaver 1988). However, Baxter et al (1989) rooted 55% of in vitro produced shoots of P. caribaea without auxin pretreatment and in nonsterile conditions. In this study, rooting in shoots not treated with auxins occurred at 24%, which did not significantly differ from those treated with 0.5p~M NAA (20%), l p~M NAA (48%), 100 I~M NAA (38%), 1 p~M NAA + 10 p~M IBA (15%), and 10 p~M IBA (18%).…”

Section: Rooting Of Adventitious Shootsmentioning

confidence: 99%

“…Berlyn et al (1987) produced adventitious buds from mature embryos of P. caribaea using half-strength Campbell and Durzan's medium with 13.3-22.2 ~M BA. Baxter et al (1989) obtained clonal plantlets of P. caribaea via axillary shoot activation in tissue culture wherein 52.5% of P. caribaea clones produced from 11 up to 1,000 explants from seven transfers per year. Using the same explant, Skidmore et al (1988) observed that P. caribaea shoots grow faster in liquid than in solid medium.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Micropropagation of Pinus caribaea Morelet

Halos

1993

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Adventitious shoot formation was induced in excised mature embryos of Pinus caribaea using a modified Murashige and Skoog medium (MSM) supplemented with 6-benzyladenine. The highest frequency (96%) of adventitious bud production was observed when embryos were exposed to 8.9 ~M BA for one week prior to transfer to a growth regulator-free medium. Increased BA concentration and longer exposure to BA significantly reduced survival rates of explants. Dilution of the basal medium to 1/4x and 1/8x decreased shoot formation but 1/2x was just as effective as full-strength. Addition of auxins, glyphosate and coconut water to the rooting medium did not improve rooting success beyond that of spontaneous rooting. Sucrose at 1.5% significantly increased rooting of shoots. Plantlets were successfully transferred to the soil after preincubation in liquid medium.

show abstract

Production of clonal plantlets of tropical pine in tissue culture via axillary shoot activation

Cited by 18 publications

References 0 publications

In vitro response of embryos from different provenances of Pinus caribaea var. hondurensis Morelet

In vitro response of embryos from different provenances of Pinus caribaea var. hondurensis Morelet

Effect of l-Phenylalanine on Rooting of Shoots from Axillary Buds of Adult Larix Leptolepis

Micropropagation of Pinus caribaea Morelet

Contact Info

Product

Resources

About