Production of Chinese rosy bitterling offspring derived from frozen and vitrified whole testis by spermatogonial transplantation

Octavera, Anna; Yoshizaki, Goro

doi:10.1007/s10695-020-00802-y

Cited by 28 publications

(11 citation statements)

References 48 publications

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“…Here, we have included both type A and type B spermatogonia in our analysis as under the right circumstances both cell types could produce viable spermatozoa in vitro. Whole testis cell suspensions have been successfully transplanted into surrogates via germ cell surrogacy 17 , 19 , 29 , 30 , but an enriched sample of spermatogonial cells, particularly type A spermatogonia, is preferred as this can improve the success rate of surrogacy experiments 31 . There are several methods for isolating spermatogonial cells from a gonadal cell suspension including gradient centrifugation 32 , 33 and elutriation 34 , fluorescent activated sorting (FACS) 14 , 35 , 36 and magnetic activated sorting (MACS) 37 – 39 .…”

Section: Discussionmentioning

confidence: 99%

“…Early stages of the germ line, specifically the oogonia and spermatogonia, are large, round cells found in the gonads of female and male fish, respectively. These cells have been successfully cryopreserved in various fish species including tench (Tinca tinca) 11 , tiger puffer (Takifugu rubripes) 12 , brown trout (Salmo trutta) 13 , rainbow trout (Oncorhynchus mykiss) 14 , manchurian trout (Brachymystax lenok) 15 , marine goby (Asterropteryx semipuctata) 16 , Siberian sturgeon (Acipenser baerii) 17 , sterlet (Acipenser rethenus) 18 and Chinese rosy bitterling (Rhodeus ocellatus ocellatus) 19 . Spermatogonia are a flexible target for biobanking compared to spermatozoa, as these cells can be collected from a fish at any life stage, without having to target adult fish during spawning periods.…”

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confidence: 99%

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Cryopreservation of testicular tissue from Murray River Rainbowfish, Melanotaenia fluviatilis

Rivers

Daly

Jones³

et al. 2020

Sci Rep

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Globally, fish populations are in decline from overfishing, habitat destruction and poor water quality. Recent mass fish deaths in Australia’s Murray–Darling Basin highlight the need for improved conservation methods for endangered fish species. Cryopreservation of testicular tissue allows storage of early sperm precursor cells for use in generating new individuals via surrogacy. We describe successful isolation and cryopreservation of spermatogonia in an Australian rainbowfish. Testis histology showed rainbowfish spermatogonia are large (> 10 μm) and stain positive for Vasa, an early germ line-specific protein. Using size-based flow cytometry, testis cell suspensions were sorted through “A” (> 9 μm) and “B” gates (2–5 μm); the A gate produced significantly more Vasa-positive cells (45.0% ± 15.2%) than the “B” gate (0.0% ± 0.0%) and an unsorted control (22.9% ± 9.5%, p < 0.0001). The most successful cryoprotectant for “large cell” (> 9 μm) viability (72.6% ± 10.5%) comprised 1.3 M DMSO, 0.1 M trehalose and 1.5% BSA; cell viability was similar to fresh controls (78.8% ± 10.5%) and significantly better than other cryoprotectants (p < 0.0006). We have developed a protocol to cryopreserve rainbowfish testicular tissue and recover an enriched population of viable spermatogonia. This is the first step in developing a biobank of reproductive tissues for this family, and other Australian fish species, in the Australian Frozen Zoo.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Cryopreservation of testicular tissue from Murray River Rainbowfish, Melanotaenia fluviatilis

Rivers

Daly

Jones³

et al. 2020

Sci Rep

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“…It has been demonstrated that sterile recipients, such as triploids (Franěk et al, 2019; Hamasaki et al, 2017; Hattori et al, 2019; Lee et al, 2013; Okutsu et al, 2007; Seki et al, 2017; Yoshikawa et al, 2017; Yoshizaki et al, 2010) and hybrids (Nagasawa et al, 2019; Wong et al, 2011; Yoshikawa et al, 2020), can produce only donor‐derived gametes in various fish species. Sterile recipients lacking endogenous germ cells as a result of gene knockdown or knockout of the dead end gene, which is required for the maintenance of primordial germ cells and their cell fate (Gross‐Thebing et al, 2017; Weidinger et al, 2003), have been particularly successful as recipients (Franěk et al, 2021; Li et al, 2017; Marinović et al, 2019; Octavera & Yoshizaki, 2019, 2020; Saito et al, 2008; Yoshikawa et al, 2020; Yoshizaki et al, 2016). In the present study, we used recipients with the greatly reduced fertility arising from hybridization, and most of the mature recipients produced only donor‐derived gametes.…”

Section: Discussionmentioning

confidence: 99%

Establishment of surrogate broodstock technology in Scombridae species by germ cell transplantation

Tani

Yazawa

Kamio

et al. 2022

Aquaculture Research

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In this study, we examined the feasibility of germ cell‐less hybrids, produced by a cross between female blue mackerel (Scomber australasicus) and male chub mackerel (S. japonicus), as recipients in order to develop a surrogate broodstock technology for mackerels, which is attracting attention as a new aquaculture target. Donor testicular cells, isolated from the testes of a chub mackerel, were transplanted into the peritoneal cavity of 500 hybrid mackerel larvae. Of the 42 male recipients that survived to 1 year of age, 35 matured and produced donor‐derived chub mackerel sperm. Amongst them, 22 produced only donor‐derived sperm, with none of the recipient's own sperm detected. Two of the six females that survived to 1‐year‐old produced donor‐derived eggs, and one of two matured females produced only donor‐derived eggs. Notably, when the mature recipients were housed in a single fish tank, some spontaneously mated, and normal hatchlings arising from fertilization of donor‐derived chub mackerel eggs and sperm were successfully obtained. Although the efficiency of this method needs to be improved in the future, hybrid mackerel can be used as surrogates for mackerel species breeding. This technology is expected to make a significant contribution to the breeding of mackerels.

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“…Purpose of the study was to provide an alternative approach for the production of donor-derived gametes of important aquaculture species. However, presented ndings could be relevant for species conservation when surrogacy can be used to propagate threatened species [14,51,52]. A close relative of gold sh-crucian carp (Carassius carassius) is facing threats of population decline because of habitat loss and concurrence from its close but invasive relative-Prussian carp (Carassius gibelio) [53].…”

Section: Discussionmentioning

confidence: 99%

Production of common carp donor-derived offspring from goldfish surrogate broodstock

Franěk

Kašpar

Gela

et al. 2020

Preprint

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Background: Common carp is the fourth most-produced species in worldwide aquaculture. Significant efforts are invested in breeding and preservation of genetic integrity of this important species. However, maintaining carp gene bank in situ can be considered as demanding due to its big body size. Recent progress in reproductive biotechnologies in fish allows improving some unfavourable characteristics of a target species using surrogate reproduction. Germ stem cells (gamete precursors) from one species are transplanted into different surrogate species with small body size. After maturation, surrogates are producing donor-derived progeny. Efficient protocols for cryopreservation of carp male and female germ stem cells have been developed lately. Thus, the next logical goal was to assess the potential of goldfish surrogate to produce donor-derived gametes of common carp after intraperitoneal transplantation of testicular cells. Results: High transplantation success was achieved when 44% of the surviving goldfish produced pure donor-derived gametes of common carp. More importantly, both viable eggs and sperm giving rise to pure common carp progeny were produced, witnessing sustainability of the presented method. Donor-derived identity of the offspring was confirmed by genotyping and typical phenotype corresponding to the donor species. Reproductive performance of chimeras was similar to goldfish controls. Assessment of gamete characteristics showed that the size of donor-derived eggs is between control carp and goldfish eggs. Interestingly, flagellum length in donor-derived spermatozoa was comparable to common carp flagellum and significantly shorter than goldfish flagellum. Conclusions: In this study, we succeeded in the production of pure common carp progeny from surrogate goldfish recipients transplanted intraperitoneally by testicular germ cells. Here we reported production of viable eggs between most distant species up to date. Good reproductive performance of goldfish germline chimeras gives a promising prospect for further analysis about the long-term reproductive performance of surrogates, recovery of cryopreserved germ cells or production of monosex stocks. Presented technology is ready to ease needs for carp breeds preservation and their recovery using many times smaller goldfish surrogates.

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Production of Chinese rosy bitterling offspring derived from frozen and vitrified whole testis by spermatogonial transplantation

Cited by 28 publications

References 48 publications

Cryopreservation of testicular tissue from Murray River Rainbowfish, Melanotaenia fluviatilis

Cryopreservation of testicular tissue from Murray River Rainbowfish, Melanotaenia fluviatilis

Establishment of surrogate broodstock technology in Scombridae species by germ cell transplantation

Production of common carp donor-derived offspring from goldfish surrogate broodstock

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