Production of chimaeric bovine embryos and calves by aggregation of inner cell masses with morulae

Picard, L.; Chartrain, Isabelle; King, W. A.; Betteridge, K.J.

doi:10.1002/mrd.1080270404

Cited by 23 publications

(10 citation statements)

References 25 publications

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“…Others have produced chimera offspring in various animal species (Cibelli et al, 1998b;Fehilly et al, 1984;Krausslich and Brem, 1985;Picard et al, 1990;Piedrahita et al, 1992;Wells and Powell, 2000). Generally, chimeric embryos were produced by aggregation of blastomeres.…”

Section: Discussionmentioning

confidence: 97%

Production of Transgenic Chimera Rabbit Fetuses Using Somatic Cell Nuclear Transfer

Matsuda

Takahashi²,

Ohkoshi³

et al. 2002

Cloning and Stem Cells

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We produced aggregate chimeric embryos between blastomeres from the somatic cell nuclear transfer (SCNT) embryos and blastomeres from normal embryos. The SCNT embryos were produced by fusing enucleated oocytes with GFP gene introduced fibroblast cells, which were derived from a day 16 fetus. GFP gene-introduced fibroblast cells were cultured and passaged four to 12 times over a period of 45-79 days before SCNT. After transferring them into pseudopregnant recipient rabbits, the 15-day postcoitus fetuses were collected. We examined the existence of the cells derived from SCNT embryos in the fetus stage of pregnancy to detect the GFP gene. Fetuses that were not collected continued to develop into newborn rabbits. Two hundred and thirty-six chimeric embryos were produced using 39 SCNT morula stage embryos, and these embryos were transferred to 11 recipient rabbits. As a result, 27 normally developed and 16 degenerated concepti were obtained. The GFP gene-positive signals were detected in one of the fetuses, two of the placentae, and two of the degenerated concepti. In this study, we found that the rabbit SCNT embryos have the ability to develop and differentiate in vivo. We also demonstrated a novel method of producing a transgenic rabbit using SCNT.

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Section: Discussionmentioning

confidence: 97%

Production of Transgenic Chimera Rabbit Fetuses Using Somatic Cell Nuclear Transfer

Matsuda

Takahashi²,

Ohkoshi³

et al. 2002

Cloning and Stem Cells

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“…Picard et al [12] reported that chimeric calves were produced from aggregates of day-8 ICM and day-5.5 morula by the method of transferring ICM cells inside the zona pellucida of diploid morula. When day-10 ICM cells, however, were used for the aggregation, no calves were chimeric.…”

Section: Discussionmentioning

confidence: 99%

In-Vitro Development of Aggregates of Bovine Inner Cell Mass Cells or Bovine Mammary Cells and Putative Tetraploid Embryos Produced by Electrofusion.

Ito

Iwasaki

1999

Journal of Reproduction and Development

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Abstract. The present study was carried out to establish aggregation procedures of bovine inner cell mass (ICM) cells and tetraploid embryos to produce cloned cattle in a different way from nuclear transfer. Bovine two-cell embryos produced by in vitro fertilization were exposed to the electric pulse to fuse 2 blastomeres resulting in tetraploid embryos. Nine (18.8%) of 48 embryos fused by electrofusion developed to blastocyst stage. Three (16.7%) aggregates consisting of two putative tetraploid embryos and one clump of ICM cells developed to blastocyst. And the aggregates which were aggregated with a clump of bovine mammary cells and a clump of ICM cells which were stained with Hoechst also developed to blastocysts. These blastocysts were illuminated by UV, overall or some parts of all ICM of the aggregates treated were observed to luminesce. This raises the possibility that the clumps of the cells can form some parts of ICM of the aggregates. These results indicate that the aggregates of a clump of ICM cells or mammary cells and tetraploid embryos developed to blastocyst stage and that some parts of ICMs of the aggregates were originated from the clump of cells.

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“…The introduction of ICM pluripotent cells into recipient embryos demonstrates that these cells contribute to the formation of mots, if not all, tissues in the fetus (Nagy et al, 1990;Picard et al, 1990). By using modified embryos that progressively contribute exclusively to placenta formation, the introduction of pluripotent cells allows the formation of viable fetuses (Nagy et al, 1990).…”

Section: Pluripotencymentioning

confidence: 99%

Lessons learned from functional assessment of pluripotency-associated transcription factors during early embryogenesis and embryonic stem cells

et al. 2017

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Pluripotency-associated transcription factors (PATF) play significant roles during early embryogenesis and in embryonic stem (ES) cells, such as control of cell-cycle progression, modulation of cellular metabolism, and transcriptional control of differentiation-inducing factors. The review aims to describe the current understanding of how these PATFs contribute to the early embryo and the ES-cell phenotypes. By a selection of representative examples of such PATFs, their roles are described, and some interesting questions are presented concerning their activity in pluripotent cells which have yet to be addressed.Keywords: embryology; pluripotent; preimplantation development; totipotency. ResumoFatores de transcrição relacionados à pluripotência (FTRP) apresentam importantes funções durante a embriogênese inicial e em células-tronco embrionárias (CTE), como o controle da progressão do ciclo celular, modulação do metabolismo celular e controle transcricional de fatores indutores de diferenciação celular. O objetivo da revisão foi descrever o conhecimento vigente sobre como estes FTRPs contribuem para o fenótipo dos embriões e CTEs. Através da seleção de exemplos representativos destes FTRPs, suas funções são descritas e algumas perguntas interessantes são apresentadas considerando suas atividades em células pluripotentes, mas que ainda não foram devidamente respondidas.Palavras-Chave: embriologia; pluripotente; desenvolvimento pré-implantacional; totipotência.

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Production of chimaeric bovine embryos and calves by aggregation of inner cell masses with morulae

Cited by 23 publications

References 25 publications

Production of Transgenic Chimera Rabbit Fetuses Using Somatic Cell Nuclear Transfer

Production of Transgenic Chimera Rabbit Fetuses Using Somatic Cell Nuclear Transfer

In-Vitro Development of Aggregates of Bovine Inner Cell Mass Cells or Bovine Mammary Cells and Putative Tetraploid Embryos Produced by Electrofusion.

Lessons learned from functional assessment of pluripotency-associated transcription factors during early embryogenesis and embryonic stem cells

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