Production of chicken anemia virus (CAV) VP1 and VP2 protein expressed by recombinant Escherichia coli

Microbial production of virus-like particle vaccine protein at gram-per-litre levels

“…Furthermore, host-cell-dependent glycosylation can concentrate near epitopes that raise neutralising antibodies (Quinones-Kochs et al, 2002). E. coli has been successfully employed to produce human papillomavirus capsid protein L1 (Chen et al, 2001;Hanslip et al, 2006), VP1 and VP2 protein of chicken anemia virus (Lee et al, 2009), VP1 of murine polyomavirus (Leavitt et al, 1985;Chuan et al, 2008a), major capsid protein VP1 of simian papovavirus SV40 (Wrobel et al, 2000), capsid protein VP1 of enterovirus type 70 (Chen et al, 2004), cowpea chlorotic mottle virus coat protein (Zhao et al, 1995) and hepatitis B virus nucleocapsid protein (Wingfield et al, 1995).…”

Development of a loop-mediated isothermal amplification method for the rapid detection of the dioxin-degrading bacterium Ochrobactrum anthropi in soil

“…Additionally, the LAMP method can be performed directly on crude biological materials, such as blood, without the need for DNA purification (Njiru et al, 2008). Recently, methods for LAMP have been improved and applied to the detection of various pathogens and genetically modified organisms (GMOs) (Lee et al, 2009). However, the LAMP is seldom applied in environmental microbiology.…”

“…Okamura et al conducted a LAMP reaction in 2008. In this test, a positive reaction was observed for Salmonella (Okamura et al, 2008). In addition, LAMP also offers a means of reducing genotyping costs as well as simplifying testing procedures (Lee et al, 2009).…”

The Development and Application of an Indirect ELISA Test for the Detection of Chicken Anaemia Virus (CAV) by VP1 in Chicken Flock Serum

“…In previous years, several expression systems have been used to express VP1-CAV, including E. coli, baculovirus-insect cells, and plant cells [21]- [24]. However, expressing full-length VP1-CAV protein has been difficult because of the cytotoxicity of the N-terminal portion of the VP1-CAV gene [25].…”