Production of CGTase by a Bacillus alkalophilic CGII strain isolated from wastewater of a manioc flour industry

Freitas, Telma Luisa de; Contiero, Jonas

doi:10.1590/s1517-83822004000200015

Cited by 17 publications

(10 citation statements)

References 28 publications

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“…The most nutrient-rich culture media increased growth of the strain, but not increased the synthesis of CGTase. Some studies, such as those conducted by de Freitas et al ( 2004 ), have reported the effect of the carbon source on the enzyme synthesis after 48 h of fermentation. According to their results, Bacillus alkalophilic CGH grew very well with higher CGTase specific activities using starch and maltodextrins as carbon sources.…”

Section: Discussionmentioning

confidence: 99%

Integral Use of Amaranth Starch to Obtain Cyclodextrin Glycosyltransferase, by Bacillus megaterium, to Produce β-Cyclodextrin

et al. 2016

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Cyclodextrin glycosyltransferase (CGTase) is an enzyme that produces cyclodextrins (CDs) from starch and related carbohydrates, producing a mixture of α-, β-, and γ-CDs in different amounts. CGTase production, mainly by Bacillus sp., depends on fermentation conditions such as pH, temperature, concentration of nutrients, carbon and nitrogen sources, among others. Bacillus megaterium CGTase produces those three types of CDs, however, β-CD should prevail. Although, waxy corn starch (CS) is used industrially to obtain CGTase and CDs because of its high amylopectin content, alternative sources such as amaranth starch (AS) could be used to accomplish those purposes. AS has high susceptibility to the amylolytic activity of CGTase because of its 80% amylopectin content. Therefore, the aim of this work was evaluate the AS as carbon source for CGTase production by B. megaterium in a submerged fermentation. Afterwards, the CGTase was purified partially and its activity to synthesize α-, β-, and γ-CDs was evaluated using 1% AS as substrate. B. megaterium produced a 66 kDa CGTase (Topt = 50°C; pHopt = 8.0), from the early exponential growth phase which lasted 36 h. The maximum CGTase specific activity (106.62 ± 8.33 U/mg protein) was obtained after 36 h of culture. CGTase obtained with a Km = 0.152 mM and a Vmax = 13.4 μM/min yielded 40.47% total CDs using AS which was roughly twice as much as that of corn starch (CS; 24.48%). High costs to produce CDs in the pharmaceutical and food industries might be reduced by using AS because of its higher α-, β- and γ-CDs production (12.81, 17.94, and 9.92%, respectively) in a shorter time than that needed for CS.

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Section: Discussionmentioning

confidence: 99%

Integral Use of Amaranth Starch to Obtain Cyclodextrin Glycosyltransferase, by Bacillus megaterium, to Produce β-Cyclodextrin

et al. 2016

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“…Moreover, some Brazilian researchers have reported the isolation and production of CGTases by Bacillus sp. (2,3,9,15,16,17,19,21). This work reports the isolation of new Bacillus sp.…”

Section: Introductionmentioning

confidence: 93%

Cyclodextrin glycosyltransferase production by new Bacillus sp. strains isolated from brazilian soil

Menocci¹,

Goulart²,

Adalberto³

et al. 2008

Braz. J. Microbiol.

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Three strains of Bacillus sp. (BACRP, BACNC-1 and BACAR) were isolated from soil adhered to cassava husk. CGTase specific activity for the three isolated strains was higher when cultivated at 40ºC. Potato starch, cassava starch, maltodextrin and glucose were used as carbon source and growth temperatures varied from 25 to 55ºC. The three isolates presented higher CGTase specific activity when cultivated with potato starch at 40ºC. Isolated BACRP and BACAR presented specific activity of 4.0x10 -3 and 2.2x10 -3 U/mg prot at pH 7.0, respectively, when cultivated in mediums added with NaCl 2%; at pH 10,0 their activities were of 3.4x10 -3 and 3.0x10 -3 U/mg prot, respectively, in the same concentration of NaCl. On the other hand, the isolated BACNC-1 presented activity specific of 2.4x10 -3 U/mg prot when cultivated at pH 7.0 added of NaCl 1%, and at pH 10.0 the specific activity was of 3.4x10 -3 U/mg prot without NaCl addition. This work also showed the presence of cyclodextrins formed during fermentation process and that precipitation with acetone or lyophilization followed by dialysis was efficient at removing CDs (cyclodextrins), thus, eliminating interference in the activity assays. The enzyme produced by the BACAR strain was partially purified and β-CD was liberated as a reaction product.

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“…The maximal CGTase production using the shake-flask method was 97.38 U mL −1 and increased to 134.05 U mL −1 in the fermentor in 72 h. The present paper reports the use of cassava starch as an alternative carbon source in a fermentor for CGTase synthesis. De Freitas et al (2004) used a range of carbon sources, such as rice, corn and soluble starch, for the synthesis of CGTase (de Freitas et al 2004). Moreover, lower concentrations of nitrogen sources were required for optimal CGTase production.…”

Section: Second Central Composite Designmentioning

confidence: 99%

Bacillus lehensis—an alkali-tolerant bacterium isolated from cassava starch wastewater: optimization of parameters for cyclodextrin glycosyltransferase production

et al. 2011

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A Gram-positive, endospore-forming, alkalitolerant bacterial strain, designated CGII, was isolated from the wastewater of a cassava flour mill in the state of São Paulo, Brazil and submitted to phylogenetic studies and biochemical tests. The 16S rRNA gene sequence indicated the highest degree of genomic similarity with MLB2 strains of Bacillus lehensis (100%). A two-level central composite rotatable design was then employed to optimize the medium composition and culture conditions for the production of the enzyme cyclodextrin glycosyltransferase (CGTase) in shake-flask and bioreactors. CCTase activity was measured under different production conditions, such as culture medium, agitation and aeration. Highest enzyme production by B. lehensis was achieved in 72 h with a maximal activity of 134.05 U mL −1 . The response surface method demonstrated that the proposed model achieved a good level of agreement with experimental data, with a correlation coefficient of 0.910, thereby confirming the adequate reliability of the model.

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Production of CGTase by a Bacillus alkalophilic CGII strain isolated from wastewater of a manioc flour industry

Cited by 17 publications

References 28 publications

Integral Use of Amaranth Starch to Obtain Cyclodextrin Glycosyltransferase, by Bacillus megaterium, to Produce β-Cyclodextrin

Integral Use of Amaranth Starch to Obtain Cyclodextrin Glycosyltransferase, by Bacillus megaterium, to Produce β-Cyclodextrin

Cyclodextrin glycosyltransferase production by new Bacillus sp. strains isolated from brazilian soil

Bacillus lehensis—an alkali-tolerant bacterium isolated from cassava starch wastewater: optimization of parameters for cyclodextrin glycosyltransferase production

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