Production of cellulolytic enzymes by Neurospora sitophila grown on cellulosic materials

Oguntimein, Gbekeloluwa B.; Vlach, D.; Moo‐Young, Murray

doi:10.1016/0960-8524(92)90217-l

Cited by 14 publications

(8 citation statements)

References 22 publications

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“…In addition to SCP, various industrial by-products have also been used as substrates for the synthesis of important microbial products, e.g., oxytetracycline production by Streptomyces rimosus using date-coats , date-seed lipid and hydrolysate (ABOU-ZEID and BAESHIN 1992); microbial enzyme production on various substrates, e.g., P-fructofuranosidase from Aspergillus japonicus grown on soybean residue, a by-product of food industry (HAYASHI et al 1992), pectinase from citrus waste by A. foetidus (HOURS and GARZON 1992), yeast polygalacturonase from dairy wastes by Kluyveromyces luctis (MURAD and FODA 1992), cellulase and P-glucosidase from corn stover/sugarcane bagasse by Neurosporu sitophila (OGUNTIMEIN et al 1992); production of citric acid using A. niger, from banana extract (SASSI et al 1991) and crude beet molasses (EL-ABYAD et al 1992); synthesis of xylitol which is widely used in the food industry as a substitute for sucrose, from sugarcane bagasse using Candida guilliermondii (MANCILHA et al 199 1) and production of xylose from xylan, a constituent of rice-straw, wheat bran, cotton-seeds, etc., by A. niger (KAWABATA et al 1992).…”

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confidence: 99%

Growth kinetics of Rhodotorula rubra on different substrates

Hari

Patel

Martı́n

1993

J. Basic Microbiol.

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The growth of a red-pigmented yeast, isolated from yogurt and identified as Rhodotorulu rubra, was studied on various industrial and agricultural by-products. Peat hydrolysate when supplemented with peptone and yeast extract supported a satisfactory growth of the yeast. Ammonium hydroxide and ammonium sulfate were the best inorganic nitrogen sources. A yield of 11.8 g/1 was obtained on molasses and wort used in aconcentration of 10 and 40% (v/v), respectively. Molasses were pre-treated to be effective. The best yield of 14 g/l, however, was obtained on a richer medium composed of molasses, peptone and yeast extract. The pH profiles were also studied and growth was found within a broad pH zone of 3 -10.

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confidence: 99%

Growth kinetics of Rhodotorula rubra on different substrates

Hari

Patel

Martı́n

1993

J. Basic Microbiol.

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“…The highest enzyme activity on 10 g l-1 substrate concentration was 0.185 FPU/ml with 48 h of cultivation ( Figure 6.a). Similarly, Oguntimein [18] also stated that the activity of cellulase enzyme produced by Neurospora on 1% corn stover and Chinese baggase was 0.15-0.18 FPU/ml. This activity also occurred in 40-60 hr of cultivation.…”

Section: Production Of Crude Cellulase Extractmentioning

confidence: 93%

Production of Bioethanol and Crude Cellulase Enyzme Extract from Napier Grass (Pennisetum purpureum S.) through Simultaneous Saccharification and Fermentation

Taufikurahman

Xie

2020

J. Biol. Sci. Technol. Man.

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Napier grass (Pennisetum purpureum S.) has been recognized for its high amounts of cellulose and hemicellulose which can be utilized for bioethanol production. Bioconversion of Napier grass to bioethanol can be performed by filamentous fungi. A fungi, Neurospora sitophila, can synthesize and secrete hydrolytic enzymes to breakdown the cellulose and hemicellulose into various monomeric sugars and simultaneously convert it to bioethanol. This study investigated the effect of Napier grass substrate concentrations and cultivation time on cellulase enzyme and bioethanol production by N. sitophila. The pretreatment of Napier grass was carried out using 1.5% (w/v) NaOH solution for 120 hours at 30 °C. After the pretreatment process, simultaneous saccharification and fermentation was conducted at 30 °C; pH 6.8-7.0 and agitation speed 130 Rpm with various of Napier grass substrate concentrations (10, 20 and 30 g l-1) and cultivation period within 24; 48; 72; 96 and 120 hours. The results show that the highest enzyme activity is 0.28 FPU/ml which was achieved at 120 hours of cultivation and 20 g/L substrate concentration of Napier grass. The highest ethanol content was obtained by 96 hours of simultaneous saccharification and fermentation (1.25 g l-1) using 20 g l-1 substrate concentration and the maximum ethanol yield is 0.30 g/g cellulose conducted at 30 g l-1 substrate concentration and 96 hours of fermentation.

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“…External calcium ion was needed to balance the calcium ion inside the cell whereas the internal calcium ion is very important for hyphal growth [33]. Neurospora sitophila has been known to be able to produce cellulase enzymes when cultivated in the submerged system as well as in the solid-state system [12,34,35]. According to Oguntimein et al in 1991, cultivation of Neurospora sitophila in a submerged fermentation system can produce cellulase enzymes with the highest activity reached 0.18 FPU after 48 hours with a corn cob as the substrate [34].…”

Section: The Effect Of Aerobic Cultivation Period On Cellulase Enzyme Activitymentioning

confidence: 99%

Bioconversion of Napier Grass Mixed with Soybean Curd Residue Producing Bioethanol through Simultaneous Saccharification and Fermentation in a Solid State Culture

Taufikurahman

Jessica

2020

J. Biol. Sci. Technol. Man.

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Napier grass is a promising lignocellulosic biomass for bioethanol production because of its high cellulose content and high annual productivity. Converting a lignocellulosic biomass into a bioethanol usually takes two steps which resulted in a long processing time and sometimes includes the utilization of hazardous chemicals. Simultaneous saccharification and fermentation in a solid-state culture using single bioconversion agent, Neurospora sitophila Shear, can reduce the overall processing time and also increase the yield of the products. The research is aimed to determine the optimum aerobic and micro-aerobic conditions that yields the highest enzyme activity and bioethanol concentration from the mixture of Napier grass and soybean curd residue. The saccharification and fermentation process was conducted in the laboratory using an incubator at 33 o C. The cellulase enzyme activity was calculated as FPAse. The highest activity achieved was 0.538 filter paper unit with the optimum mass ratio of Napier grass to soybean curd residue was 1:1 on the second day of cultivation period. The fermentation process was conducted aerobically for two days and then followed by six days of micro-aerobic fermentation, resulting in the highest bioethanol yield of 2.12% (w/w) at the end of the culture period. The optimum mass ratio was found to be 9:1. This study shows that Napier grass and simultaneous saccharification and fermentation method has a great potential for cellulase and bioethanol production, but further improvement on the micro-aerobic system is needed to maximize the bioethanol yield.

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Production of cellulolytic enzymes by Neurospora sitophila grown on cellulosic materials

Cited by 14 publications

References 22 publications

Growth kinetics of Rhodotorula rubra on different substrates

Growth kinetics of Rhodotorula rubra on different substrates

Production of Bioethanol and Crude Cellulase Enyzme Extract from Napier Grass (Pennisetum purpureum S.) through Simultaneous Saccharification and Fermentation

Bioconversion of Napier Grass Mixed with Soybean Curd Residue Producing Bioethanol through Simultaneous Saccharification and Fermentation in a Solid State Culture

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