Production of Cellulases and Xylanases by Trichoderma viride and Biological Processing of Lignocellulose and Recycled Paper Fibers

Viesturs, U.; Leite, Mônica Batista; Treimanis, A.; Eremeeva, T.; Apsite, A.; Eisimonte, M.; Jansons, P.

doi:10.1007/978-1-4612-0223-3_30

Cited by 8 publications

(5 citation statements)

References 14 publications

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“…Endoglucanases are more active on amorphous cellulose and randomly attack the inner part of the cellulosic chain, whereas exoglucanases can hydrolyse both crystalline and amorphous cellulose by removing cellobiose from the terminal part of the cellulose chains (Henrrissat, 1994). Although considered more detrimental to fibres, endoglucanase action is probably the main determinant of drainage improvement (Jackson et al, 1993;Stork and Puls, 1994;Viesturs et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Enzymatic Modification of Paper Fibres

Pala

Мота

Gama

2002

Biocatalysis and Biotransformation

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The enzymatic mode of action in paper fibre upgrading is still uncertain. In an attempt to clarify how enzymes modify pulp and paper properties, several parameters were analysed in the present work: (i) thermal analysis of the water -solid surface interactions; (ii) fibre cake permeability; (iii) particle size analysis. The results obtained suggest that enzymes modify the interfacial properties of fibres, increasing the water affinity, which in turn change the technical properties of pulp and paper, such as drainability and strength. The modification of paper and pulps following a treatment with cellulosebinding domains further supports this hypothesis.

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Section: Introductionmentioning

confidence: 99%

Enzymatic Modification of Paper Fibres

Pala

Мота

Gama

2002

Biocatalysis and Biotransformation

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“…There has been a great interest in the commercial use of cellulases in the agricultural and chemical industries to produce glucose for feed, food, and the manufacture of other chemicals (9)(10)(11)(12). Deinking and fiber modification in the paper industry, as well as biopolishing in the textile industry, are less known but not less important applications (13)(14)(15)(16)(17).…”

Section: Introductionmentioning

confidence: 99%

Production, Purification, and Properties of an Endoglucanase Produced by the HyphomyceteChalara(Syn.Thielaviopsis)paradoxaCH32

Lucas

Robles

Garcı́a

et al. 2000

J. Agric. Food Chem.

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The hyphomycete Chalara (syn. Thielaviopsis) paradoxa produces endoglucanase activity during the late trophophase. The low molecular mass (35 kDa) endoglucanase purified from cultured broths works optimally at 37 degrees C and pH 5.0. The enzyme inactivates at pH below 3.0 and also at temperatures of 50 degrees C or higher, but it is stable at lower temperatures, including refrigeration temperature and freezing. The enzyme is inhibited by detergents, by EDTA, and by the divalent cations Hg(2+) and Ag(2+). It is also inhibited to some extent by 10 mM Zn(2+), Fe(2+), and Mg(2+), but it is stimulated by Mn(2+). Enzyme activity is not affected by reducing agents. In the presence of low concentrations of water miscible organic solvents (20%) endoglucanase activity is inhibited by 7% (for methanol) to 50% (for acetonitrile), and it is totally inhibited at higher solvent concentrations (50%). Enzyme activity is not affected by the water immiscible solvent ethyl acetate. Carboxymethylcellulose is the preferred substrate (K(m(app)) = 8.3 g/L; V(max(app)) = 1.1 microM/min). Hydrolysis of crystalline cellulosic substrates is very limited, but it is greatly enhanced by phosphoric acid swelling. The purified enzyme shows no activity toward disaccharides or aryl-glucosides. Its activity is inhibited by cellobiose.

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“…Straw used for composting, after preliminary digestion of the external wax layer, provides easily available polysaccharides for cellulolytic enzymes secreted by microorganisms [75]. Trichoderma belongs to fungi known for their ability to degrade cellulose [76]. The presence of antagonistic fungi in composted sawdust was also reported by Eida et al [77].…”

Section: Mutual Relations Between Populations Of Potentially Phytopatmentioning

confidence: 89%

Composts and Water Extracts of Lignocellulosic Composts in the Aspect of Fertilization, Humus-Forming, Sanitary, Phytosanitary and Phytotoxicity Value Assessment

Bohacz

2018

Waste Biomass Valor

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Purpose Lignocellulosic biomass waste is produced in large quantities and only a small part is used for utility purposes. The aim of the work was to assess the fertilization value of the solid fraction and water extract of lignocellulosic composts. Methods Compost I contained 42.86% pine bark, 34.28% grass, 20.0% sawdust and 2.86% chicken feathers; compost II contained 25.54% pine bark, 10.63% wheat straw, 51.07% sawdust and 12.76% chicken feathers. Seldom described physical properties and phytosanitary and sanitary status of composts as well as macro-and micronutrients and heavy metals concentrations were determined in the solid fraction and water extracts of composts and their impact on the germination of Lepidium sativum L. using PC analysis. Results Composts were characterized by good physical properties and a light product, safe phytosanitary status, which was related to the prevalence of potentially antagonistic fungi, especially in compost II. The frequency of Aspergillus fumigatus, considered an opportunistic human pathogen, decreased during the composting. Heavy metal concentrations did not exceed permissible values for improvers and cultivation media in the solid and aqueous compost fractions. The tested composts were sanitary safe. Both composts were non-toxic to plants, because the germination index in compost I was PCA analysis demonstrated that the majority of micro-and macronutrients and heavy metals had a significantly negative effect on root elongation and germination of the test plant (cress) seeds, especially in compost I. Conclusions Lignocellulosic waste after composting is a valuable and safe organic fertilizer.

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Production of Cellulases and Xylanases by Trichoderma viride and Biological Processing of Lignocellulose and Recycled Paper Fibers

Cited by 8 publications

References 14 publications

Enzymatic Modification of Paper Fibres

Enzymatic Modification of Paper Fibres

Production, Purification, and Properties of an Endoglucanase Produced by the HyphomyceteChalara(Syn.Thielaviopsis)paradoxaCH32

Composts and Water Extracts of Lignocellulosic Composts in the Aspect of Fertilization, Humus-Forming, Sanitary, Phytosanitary and Phytotoxicity Value Assessment

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