Production of cellulase/β-glucosidase by the mixed fungi culture Trichoderma reesei and Aspergillus phoenicis on dairy manure

Wen, Zhiyou; Liao, Wei; Chen, Shulin

doi:10.1016/j.procbio.2005.03.044

Cited by 146 publications

(78 citation statements)

References 24 publications

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“…In a study by Juhász et al (2005), Trichoderma reesei and different use of waste as a carbon source, such as cellulose delignified, in treated willow, conifer and corn cob showed determinants that are sources for the production of β-glucosidase. However, the production of β-glucosidase by the genus Trichoderma is not as high as to Aspergillus phoenicis, which enhances the ability of this enzyme as a producer and the use of mixed cultures for the production of enzymes (Duff et al, 1986;Wen et al, 2005). As the genus Trichoderma, the species L. edodes does not seem to be a good producer of β-glucosidase enzyme.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of hollocelulase production by Lentinula edodes (Berk.) Pegler during the submerged fermentation growth using RSM

et al. 2014

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The cellulase proteins have a great importance in the enzymatic hydrolysis of woody biomass. Despite of costs being a major concern, it has been a stimulus to study basidiomycetes biochemical properties which degrade lignocellulosic material and have prompted the processes' study for obtaining cellulolytic enzymes in fungi. The objective of this research was to evaluate the effects of the initial nitrogen content on (ammonium sulfate) and on sugar cane bagasse, which hereby, acts as an inducer of hydrolytic enzymes to produce cellulases and xylanases, using three Lentinula edodes (Berk.) Pegler strains as a transformation agent. A factorial design with 2 2 replications in the central point was conducted, varying concentrations of ammonium sulfate and sugar cane bagasse. The submerged cultures carried out in synthetic culture medium and incubated at 25°C for 7 days on an orbital shaker at 150 rpm. The total protein and cellulase activity as endoglucanase, exoglucanase and β-glucosidase and the xylanase was also determined. The results showed that the production of hydrolytic enzymes was stimulated by the presence of high concentrations of sugar cane bagasse (30g/L), characterizing it as an inducer due to the demonstrated proportional relationship. Thus, ammonium sulfate acted as a reducing agent in the synthesis of enzymes, being the low concentrations (0.1g/L) indicated for the enzyme production system under study. Among the studied strains, the EF52 showed higher activity for xylanase, endoglucanases, β-glucosidase and also protein.Keywords: basidiomycetes, sugar cane bagasse, ammonium sulfate, hydrolytic enzymes, proteins. Avaliação da produção de enzimas holocelulolíticas por Lentinula edodes (Berk.) Pegler durante o crescimento em fermentação submersa usando MSR ResumoAs celulases são proteínas de grande importância na hidrólise enzimática de biomassa florestal. No entanto, seu custo elevado tem estimulado o estudo de processos de obtenção de enzimas celulolíticas por fungos filamentosos, tais como os basidiomicetos que apresentam propriedades bioquímicas para degradação de material lignocelulósico. O objetivo deste trabalho foi avaliar os efeitos do teor inicial de nitrogênio (sulfato de amônia) e de um indutor de enzimas hidrolíticas (bagaço de cana de açúcar) na produção de xilanases e celulases utilizando três isolados de Lentinula edodes (Berk.) Pegler como agente de transformação. Foi realizado um planejamento fatorial 2 2 com repetição no ponto central, variando as concentrações de sulfato de amônia e bagaço de cana de açúcar. O cultivo submerso realizado em meio de cultivo sintético e incubado a 25°C por 7 dias em agitador orbital a 150 rpm. Foram determinados o teor de proteínas totais e a atividade de celulase como: endoglucanase, exoglucanase e β-glucosidase e ainda xilanase. Os resultados demonstraram que a produção das enzimas hidrolíticas foi estimulada pela presença de alta concentração de bagaço de cana (30g/L), caracterizando-o como agente indutor devido à relação de proporcionalidad...

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Section: Discussionmentioning

confidence: 99%

Evaluation of hollocelulase production by Lentinula edodes (Berk.) Pegler during the submerged fermentation growth using RSM

et al. 2014

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“…[4][5][6] The cellulase produced by fungi has three major enzyme components: endoglucanases, which randomly hydrolyze internal glycosidic linkages; cellobiohydrolases, which produce cellobiose from cellulose chain ends; and -glucosidases, which convert cellobiose into glucose. 6) The filamentous fungus, Acremonium cellulolyticus, which was isolated in 1982 from soil in Japan, is a cellulose-degrading organism.…”

Section: Isolation Of Uracil Auxotrophs Of the Fungus Acremonium Cellmentioning

confidence: 99%

Isolation of Uracil Auxotrophs of the FungusAcremonium cellulolyticusand the Development of a Transformation System with thepyrFGene

Fujii¹,

Iwata²,

Murakami³

et al. 2012

Bioscience, Biotechnology, and Biochemistry

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“…The cost of enzymes is currently major factor limiting the commercialization on the enzymatic deglycosylation of natural botanical active ingredients for industrial production [25]. Aspergillus species are efficient producers of cellulase [26,27]. Cellulase is a multi-component carbohydrase presenting endo-1,4-β-glucanase (EC 3.2.1.4), exo-1,4-β-glucanase (EC 3.2.1.91) and β-glucosidase (EC 3.2.1.21) activities, which breaks down cellulose through synergistic action [28,29].…”

Section: Introductionmentioning

confidence: 99%

Production of Resveratrol by Piceid Deglycosylation Using Cellulase

et al. 2016

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Abstract:Resveratrol is a dietary polyphenolic compound widely used in medicine, food, and cosmetic products. The glycoside form of resveratrol, piceid, is also present in several plant materials but is less bioavailable. In this study, enzymatic transformation of piceid into resveratrol using inexpensive cellulase was investigated. Response surface methodology was used to evaluate the effect of reaction parameters, including reaction temperature, reaction time, enzyme amount and pH. The optimal conditions for biotransformation of piceid to resveratrol are: a reaction temperature of 50˝C, reaction time of 4.75 h, enzyme amount of 2.5 fungal β-glucanase (FBG) units and pH of 4.3. In addition, the extracts from Polygonum cuspidatum root contained high amounts of piceid were treated with cellulase in order to deglycosylation that increased resveratrol yield. After treatment, the resveratrol yield significantly increased from 2.72 to 9.49 mg/g, while the piceid contents decreased from 8.60 to 0 mg/g. The result provides an efficient method to convert piceid in the extracts of P. cuspidatum root into resveratrol by cellulase.

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Production of cellulase/β-glucosidase by the mixed fungi culture Trichoderma reesei and Aspergillus phoenicis on dairy manure

Cited by 146 publications

References 24 publications

Evaluation of hollocelulase production by Lentinula edodes (Berk.) Pegler during the submerged fermentation growth using RSM

Evaluation of hollocelulase production by Lentinula edodes (Berk.) Pegler during the submerged fermentation growth using RSM

Isolation of Uracil Auxotrophs of the FungusAcremonium cellulolyticusand the Development of a Transformation System with thepyrFGene

Production of Resveratrol by Piceid Deglycosylation Using Cellulase

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