Production of cellulase in solid-state fermentation withTrichoderma reesei MCG 80 on wheat straw

Chahal, Parminder S.; Chahal, D. S.; Lê, George B. B.

doi:10.1007/bf02941724

Cited by 27 publications

(3 citation statements)

References 11 publications

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“…Proximal analyses of mango industrial wastes (Table 1) suggested that they are both suitable and sustainable substrates for raising microorganisms of agricultural importance. Similar findings have been reported for wastes of cabbage, wheat straw, banana, corncob, and grapes (Chahal et al 1996;Ojumu et al 2003;Baig 2005;Bai et al 2008;Das and Ghosh 2009). Mango industrial wastes are largely composed of nitrogenfree extract indicating high contents of soluble and available carbohydrates suitable for supporting the growth of fungi such as T. asperellum T8a.…”

Section: Proximal Analyses Of Mango Industrial Wastes and T Asperellu...supporting

confidence: 82%

PRODUCTION OF Trichoderma asperellum T8a SPORES BY A “HOME-MADE” SOLID-STATE FERMENTATION OF MANGO INDUSTRIAL WASTES

Santos-Villalobos¹,

Hernández-Rodríguez²,

Villaseñor-Ortega³

et al. 2012

BioResources

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Dry wastes (dw) generated in processing mangoes, composed (in dry weight) mainly of soluble carbohydrates (71 ± 2%) and fiber (16 ± 1%), were evaluated as substrates in a "home-made" solid-state fermentation (using polyurethane foam as inert support matrix, various C:N ratios, moisture contents, and incubation periods) of Trichoderma asperellum T8a, a promising biological control agent against the mango pathogen Colletotrichum gloeosporioides (causal agent of anthracnose). Highest spore production (2.5 x 10 6 up to 76 ± 3 x 10 8 spores g -1 dw) occurred after 8 days of incubation [at 28 ± 1 °C, relative humidity of 85 ± 5%, photoperiod of 12h (540 Lux) -12h (20 Lux)] at a C:N ratio of 26, and a moisture content of 78%. Scanning electron microscopy showed that T. asperellum T8a was able to grow on mango industrial wastes and into polyurethane foam. The extensive growth can be related to cellulases secreted by this fungus, liberating glucose from these wastes to its growth. Most (94 ± 1%) of the spores grown on mango industrial wastes survived storage at 4 °C for 7 days and were equally effective as those grown on potato dextrose agar medium (86 ± 4% viable) in biological control tests against C. gloeosporioides ATCC MYA 456. Results indicate the potential use of mango industrial wastes as substrates to produce T. asperellum T8a spores in situ (mango orchards) under a cheap "homemade" solid-state fermentation, reducing problems associated with wastes disposal and permitting the production of a biological control agent against C. gloeosporioides.

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Section: Proximal Analyses Of Mango Industrial Wastes and T Asperellu...supporting

confidence: 82%

PRODUCTION OF Trichoderma asperellum T8a SPORES BY A “HOME-MADE” SOLID-STATE FERMENTATION OF MANGO INDUSTRIAL WASTES

Santos-Villalobos¹,

Hernández-Rodríguez²,

Villaseñor-Ortega³

et al. 2012

BioResources

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“…A popular producer of cellulase wthis still Trichoderma sp (15, 16, 19, 20). Using this strain corn fiber (19), wheat straw (22), waste newspaper and sorbose, or manure (17, 24) were used for cellulase production. Penicillium brasilianum (18) or P. janthinellum (21) have also reported for cellulase production.…”

Section: Discussionmentioning

confidence: 99%

Efficient Cellulase Production by the Filamentous Fungus Acremonium cellulolyticus

Ikeda

Hayashi

Okuda³

et al. 2007

Biotechnology Progress

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Cellulase production was investigated in a culture of a strain of Acremonium cellulolyticus. The medium components were optimized for the improvement of cellulase production. The maximum production of cellulolytic enzymes was obtained in a medium containing (grams per liter) 50 Solka Floc, 5 (NH4)2SO4, 24 KH2PO4, 4.7 potassium tartrate hemihydrate, 1.2 MgSO4.7H2O, 1 Tween 80, 4 urea, 0.01 ZnSO4.7H2O, 0.01 MnSO4.6H2O, and 0.01 CuSO4.7H2O, with a pH of 4.0. In the flask culture, 15.5 filter paper units (FPU)/mL of maximum cellulase activity was obtained, 17.42 FPU/mL in a 7-L bioreactor, and 13.08 FPU/mL in a 50-L scale bioreactor for 4-8 d at 30 degrees C. Average production rates were 1.94 FPU/mL.d in flasks, 2.86 FPU/mL.d in the 7-L bioreactor, and 2.56 FPU/mL.d in the 50-L bioreactor. Cellulase production on a small scale was successfully reproduced in the 50-L pilot scale bioreactor. Saccharification activity from A. cellulolyticus was compared with cellulolytic enzymes produced by other strains. The A. cellulolyticus culture broth had a comparable saccharification yield in comparison with those of other Trichoderma enzymes (GC220 or Cellulosin T2) under the same total cellulase activity. Its saccharification yield (percent of released reducing sugar to used dried substrate) was 60%, and its glucose content was 83%.

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“…This is because SmF systems offer easier sterilisation and involve a less-laborious process. Nevertheless, to produce microbial enzymes at a lower operating cost and capital investment, solid-state fermentation (SSF) could be an alternative system [10][11][12][13]. Since SmF utilises free-flowing liquid substrates, it would be the best method for bacterial work as bacteria require a high moisture content, unlike SSF, which is implemented in the absence of freeflowing water [9,14].…”

Section: Introductionmentioning

confidence: 99%

Optimisation of Xylanase–Pectinase Cocktail Production with Bacillus amyloliquefaciens ADI2 Using a Low-Cost Substrate via Statistical Strategy

et al. 2022

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An effective statistical tool for increasing and boosting the production of xylanase and pectinase by Bacillus amyloliquefaciens ADI2 during submerged fermentation (SmF) appears to be the response of surface methodology (RSM) using the central composite design (CCD). Optimum production was achieved under fermentation conditions of a temperature of 28 °C, pH of 8.38, inoculum size of 4% (w/v) and agitation speed of 94 rpm for 48 h. The experimental responses demonstrated a near agreement with the expected responses under optimum conditions of independent variables, suggesting the model’s validity. The optimised CCD model had a 1.34-fold, 159 ± 6 U/mL greater xylanase and 5.96-fold, 205 ± 9 U/mL greater pectinase production than the one factor at a time (OFAT) approach. The production of concurrent enzymes of xylanase–pectinase resulted in a ratio of 1:1.3.

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Production of cellulase in solid-state fermentation withTrichoderma reesei MCG 80 on wheat straw

Cited by 27 publications

References 11 publications

PRODUCTION OF Trichoderma asperellum T8a SPORES BY A “HOME-MADE” SOLID-STATE FERMENTATION OF MANGO INDUSTRIAL WASTES

PRODUCTION OF Trichoderma asperellum T8a SPORES BY A “HOME-MADE” SOLID-STATE FERMENTATION OF MANGO INDUSTRIAL WASTES

Efficient Cellulase Production by the Filamentous Fungus Acremonium cellulolyticus

Optimisation of Xylanase–Pectinase Cocktail Production with Bacillus amyloliquefaciens ADI2 Using a Low-Cost Substrate via Statistical Strategy

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