Production of cellulase-free xylanase by Trichoderma reesei SAF3

Kar, Sanjay Kumar; Mandal, Asish; Mohapatra, Pradeep Kumar Das; Mondal, Keshab Chandra; Pati, Biswamoy

doi:10.1590/s1517-83822006000400011

Cited by 33 publications

(21 citation statements)

References 7 publications

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“…The enzyme titre declined with increase in inoculum size beyond 1.0%. Several researchers have reported the use of 1.0-5.0% (v/v) inoculum for hyperproduction of xylanase [6,22,43]. Further, high concentration of inoculum is not preferred in industrial fermentation.…”

Section: Inoculum Size and Agementioning

confidence: 99%

Production and optimization of cellulase-free, alkali-stable xylanase by Bacillus pumilus SV-85S in submerged fermentation

Nagar

Gupta

Kumar

et al. 2009

J Ind Microbiol Biotechnol

113

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This paper reports the production of a cellulase-free and alkali-stable xylanase in high titre from a newly isolated Bacillus pumilus SV-85S using cheap and easily available agro-residue wheat bran. Optimization of fermentation conditions enhanced the enzyme production to 2995.20 +/- 200.00 IU/ml, which was 9.91-fold higher than the activity under unoptimized basal medium (302.2 IU/ml). Statistical optimization using response-surface methodology was employed to obtain a cumulative effect of peptone, yeast extract, and potassium nitrate (KNO(3)) on enzyme production. A 2(3) central composite design best optimized the nitrogen source at the 0 level for peptone and yeast extract and at the -alpha level for KNO(3), along with 5.38-fold increase in xylanase activity. Addition of 0.1% tween 80 to the medium increased production by 1.5-fold. Optimum pH for xylanase was 6.0. The enzyme was 100% stable over the pH range from 5 to 11 for 1 h at 37 degrees C and it lost no activity, even after 3 h of incubation at pH 7, 8, and 9. Optimum temperature for the enzyme was 50 degrees C, but the enzyme displayed 78% residual activity even at 65 degrees C. The enzyme retained 50% activity after an incubation of 1 h at 60 degrees C. Characteristics of B. pumilus SV-85S xylanase, including its cellulase-free nature, stability in alkali over a long duration, along with high-level production, are particularly suited to the paper and pulp industry.

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Section: Inoculum Size and Agementioning

confidence: 99%

Production and optimization of cellulase-free, alkali-stable xylanase by Bacillus pumilus SV-85S in submerged fermentation

Nagar

Gupta

Kumar

et al. 2009

J Ind Microbiol Biotechnol

113

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“…La enzima xilanasa en cuya producción industrial se utiliza principalmente el hongo Trichoderma sp., cataliza la hidrolisis del xilano polisacárido que constituye principalmente junto con la celulosa la hemicelulosa de la pared vegetal. Esta enzima se utiliza extensivamente en los procesos de alimentos y en la industria química y de pulpa, en procesos de obtención de biopulpa y de bioblanqueado, ya que provee ventajas como descenso en el consumo de cloro, la demanda química de oxigeno, con el consecuente incremento de la calidad del agua residual proveniente del proceso, (Kar et al, 2006).…”

Section: Universidad Eanunclassified

Implementación e innovación en biotecnología ambiental e industrial en el contexto empresarial colombiano

Rincón¹,

Emilia²

2011

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“…A exploração dos micro-organismos pela indústria gera bilhões de dólares a cada ano [6] e as vantagens de realizar ensaios com fungos filamentosos se devem a fatores como fácil cultivo e manipulação, produção de altos níveis de enzimas extracelulares, maior especificidade, menor consumo energético e maior velocidade de reação [7][8][9].…”

Section: Introductionunclassified

Produção de enzimas extracelulares por fungos associados à decomposição de materiais vegetais em riachos

Silva

Frantz²,

Marques³

et al. 2015

J. Bioen. Food Sci.

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As celulases e xilanases fúngicas, derivadas de sua habilidade degradativa e excretadas, em geral, para o meio, despertam interesse biotecnológico por terem um largo espectro de aplicação. Objetivou-se verificar a produção das enzimas celulase e xilanase por fungos filamentosos isolados de detritos foliares alóctone em um córrego no Cerrado. Os testes foram realizados com 100 cepas utilizando meio de cultura sintético contendo, como única fonte de carbono, carboximetilcelulose e xilana para celulase e xilanase, respectivamente. Foram realizados inóculos no centro das placas e armazenadas por 4 dias em BOD a 28°C e submetido a choque térmico em estufa a 50°C por 16 horas. Para melhor visualização do halo de hidrólise as placas foram coradas com solução de vermelho congo e lavadas com solução de NaCl. Dos fungos testados 69% apresentaram resultado positivo para celulase dos quais 23% foram consideramos com potencial para aplicação em biotecnologia. Não houve resultados positivos para a produção da enzima xilanase. Os fungos testados, associados ao processo de decomposição de matéria orgânica vegetal, apresentaram produção da enzima celulase, mas não da enzima xilanase. ABSTRACT-Fungal cellulases and xylanases derived from the huge degradative capabilities of these microorganisms and excreted to medium, raise a biotechnologixcal interest due to large application potential. The objective of this paper is to identify the production of the enzymes cellulase and xylanase by filamentous fungi isolated from allochthonous leaf litter in a stream in Cerrado ecosystem. The tests were carried out using 100 strains grown in synthetic culture medium containing carboxymethyl cellulose or beechwood xylan as sole carbon source for cellulase and xylanase production detection respectively. Inocula were performed in the center of plates and stored for 4 days at 28 ° C and subjected afterwards to thermal shock at 50 ° C for 16 hours. Plates were stained with Congo red solution and washed with a NaCl solution for betterdetection of the hydrolysis halo. Sixty nine percent of the fungal strains tested were positive for cellulase of which 23% were considered with potential for application in biotechnology. None of the fungal strains was able to degrade xylan.

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Production of cellulase-free xylanase by Trichoderma reesei SAF3

Cited by 33 publications

References 7 publications

Production and optimization of cellulase-free, alkali-stable xylanase by Bacillus pumilus SV-85S in submerged fermentation

Production and optimization of cellulase-free, alkali-stable xylanase by Bacillus pumilus SV-85S in submerged fermentation

Implementación e innovación en biotecnología ambiental e industrial en el contexto empresarial colombiano

Produção de enzimas extracelulares por fungos associados à decomposição de materiais vegetais em riachos

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