Production of cellulase by Trichoderma reesei from dairy manure

Wen, Zhiyou; Liao, Wei; Chen, Shulin

doi:10.1016/j.biortech.2004.05.021

Cited by 171 publications

(95 citation statements)

References 27 publications

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“…The enzymatic hydrolysis of cellulosic materials has been widely studied (Okafoagu and Nzelibe, 2006;Wen et al, 2005 ;Foyle et al, 2007). The production of sugars, biofuels and single cell protein are potential uses of such hydrolysis.…”

Section: Discussionmentioning

confidence: 99%

Physico-chemical properties of municipal refuse in Lagos metropolis and cellulolytic activities of resident microorganisms associated with organic matter degradation

Ogunyemi¹,

Amund²,

Okpuzor³

et al. 2010

International Journal of Biological and Chemical Sciences

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This study investigated the activities of micro organisms involved in the degradation of organic matter in solid wastes and their potential to produce cellulolytic enzymes. Soil samples of decomposing waste piles were collected from four designated landfill sites in Lagos metropolis and analyzed for physicochemical properties, toxic heavy metal content and microbial populations. Findings revealed that the moisture content of the soils ranged from 7.6 -10.0% in all the locations sampled. Ojota-Olusosun site had the highest organic matter content of 10.65%. The highest viable bacterial counts were 28.2 ± 3.0 x 10 6 cfu/g while fungi were 47.0 ± 4.0 x 10 4 cfu/g respectively. A similar pattern was observed for phosphate and chloride levels while some heavy metals were also detected in varying and high amounts. There was a significant positive correlation at 5% level between fungal viable counts and phosphate ion while a significant negative correlation was observed for total hydrocarbon. The bacteria associated with the soil samples were identified as Escherichia coli, Bacillus spp, Klebisella spp, Micrococcus spp. and Acinetobacter spp while the resident fungal species were mostly the Aspergillus spp and an isolate identified as Mucor spp. The moulds were found to be capable of utilising lignin and cellulosic substrates for growth and for production of cellulolytic enzymes. Results from this study suggest that such micro organisms could be useful in bioconversion of cellulosic substrates and solid wastes to cellulolytic enzymes for industrial processes.

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Section: Discussionmentioning

confidence: 99%

Physico-chemical properties of municipal refuse in Lagos metropolis and cellulolytic activities of resident microorganisms associated with organic matter degradation

Ogunyemi¹,

Amund²,

Okpuzor³

et al. 2010

International Journal of Biological and Chemical Sciences

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“…As it was mentioned that T. reesei was one of the best fungi to produce cellulase enzyme when using solid media method [12] . Also others confirmed that the fungi Trichoderma species is one of the most productive fungal of the enzyme cellulase [15] .…”

Section: Resultsmentioning

confidence: 99%

“…All the above components of the media were dissolved in distilled water except, urea. The prepared media was adjusted to a pH 6.0 and distributed as (50 ml) in each conical flasks size (250 ml), closed tightly with cotton, wrapped with aluminum foil and sterilized under the same above condition [12] .…”

Section: Solid Mediamentioning

confidence: 99%

Evaluation of Enzyme Cellulase Production and it's Activity, Isolated from Local Fungi

2017

IJSR

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Eight Fungal species were isolated from the rhizosphere of wheat Triticum aestivum ( Trichoderma harzianum, T.viride, T.longibrachiatum, Alternaria alternate, Fusarium solan, Aspergillus niger, Rhizoctonia solani and Fusarium oxysporum). All the isolated fungi had the capacity to cellulase enzyme production in solid media (CMC-Agar), moreover, T.viride gave the highest capability for the production of the enzyme (6.14). Rhizoctonia solani gave less productive ratio (3.0). Quantitative test using Mandel liquid media showed that the fungus T.viride had the highest activity of cellulase enzyme (4.39 U/ml), however this fungus had the greatest biomass (8.96 g/L). The fungus Rhizoctonia solani gave lower enzyme activity (2.04 U/ml) and its biomass reached to (4.65 g/L). Studying of different carbon sources for the fungus T. viride showed that sucrose was the best carbon source used with the liquid media for cellulase activity (3.87 U/ml), and the biomass (2.83 g/L). From these findings, such fungi able to produce enzymes when activated depending on the substrate and cultured media.

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“…Some cellulases exist as discreate multienzyme complexes, called cellulosomes which consists of multiple subunits cellulolytic enzymes on the other hand of filamentous fungi is non -complexed that consists of exoglucanases or cellobiohydrolases (CBH I and CBH II). Enzymes synergistically act to catalyze the hydrolysis of cellulose (Wen et al, 2005). Cellulose units are interlinked by β -1, 4-glycosidic bonds is a liner polymer of D-glucose units, which is renewable biomass available on earth.…”

Section: Introductionmentioning

confidence: 99%

“…Degradation of hemicellulose, converts hemicellulose into valuable saccharides, is done by interdependent and synergistic hemicellulases. Xylan, β-glucan, polygalacturonan, xyloglucan, arabinoxylan, mannan, galactan, galactomannan, arabinan, etc are comman hemiicelluloses, they are targets of xylanase, β-glucanase, polygalacturonase, xyloglucanase, mannanase, arabinase, galactanase, glucuronidase, acetyl xylan esterase, and other enzymes (Wen et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Mutagenesis and screening of Penicillium oxalicum (Dal 5) for enhancing cellulase production

2020

IJMR

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In the modern era due to increasing fuel demand, prices of crude oil is increasing day by days. So the need for bioenergy sources is increasing in coming years. Of all the biomass present in the world lignocelluloses as industrial, forest and agricultural residues constitutes the major portion. The present study was focused to determine potential of fungus Penicillium oxalicum (Dal 5) for cellulase production on different commonly available lignocellulosic substrates (Rice straw, Wheat straw, Corn cob, Baggase, Sorghum straw), organic nitrogen source (Corn steep liquor).

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Production of cellulase by Trichoderma reesei from dairy manure

Cited by 171 publications

References 27 publications

Physico-chemical properties of municipal refuse in Lagos metropolis and cellulolytic activities of resident microorganisms associated with organic matter degradation

Physico-chemical properties of municipal refuse in Lagos metropolis and cellulolytic activities of resident microorganisms associated with organic matter degradation

Evaluation of Enzyme Cellulase Production and it's Activity, Isolated from Local Fungi

Mutagenesis and screening of Penicillium oxalicum (Dal 5) for enhancing cellulase production

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