Production of cellulase and xylanase by a wild strain of Trichoderma viride

Abstract

The production of cellulase and xylanase was investigated with a newly isolated strain of Trichoderma viride BT 2169. The medium composition was optimized on a shake-flask scale using the Graeco-Latin square technique. The temperature and time for optimal growth and production of the enzymes in shake cultures were optimized using a central composite design. The temperature optima for maximal production • of filter paper cellulase (FPase), xylanase and fl-gluosidase were 32.8 °, 34.7 ° and 31.1 ° C, respectively, and the optimum times for production of these enzymes were found to be 144, 158 and 170 h, respectively. The optimized culture medium and conditions (33°C) gave 0.55 unit of FPase, 188.1 units of xylanase and 3.37 units of fl-glucosidase per milliliter of culture filtrate at 144 h of shake culture. Among different carbon sources tested, the maximum enzyme activities were produced with sulphite pulp and all three enzymes were produced irrespective of the carbon sources used. Batch fermentation in a laboratory fermentor using 2% sulphit e pulp allowed the production of 0.61 unit of FPase, 145.0 units of xylanase and 2.72 units of fl-glucosidase. In a fed-batch fermentation on 6% final Avicel concentration FPase and fl-glucosidase were 3.0 and 2.4 times higher respectively than those in batch fermentation on 2% Avicel. The pH and temperature optima as well as pH and temperature stabilities of T. viride enzymes were found to be comparable to T. reesei and some other fungal enzymes.

Investigation of factors affecting xylanase activity from Trichoderma harzianum 1073 D3

“…Similarly, in other studies carried out with Trichoderma sp. it was concluded that the optimum temperature varied between 45 and 60°C (Dekker, 1983;Gomes et al, 1992). Besides, for fungal xylanases the optimum temperature was 60°C (Chandra and Chandra, 1995;Chandra and Chandra, 1996;Gandhi et al, 1994).…”

“…Our studies revealed that the xylanase activity was maximum around pH 5. In studies carried out with Trichoderma sp., it was also concluded that the most suitable pH value for xylanase activity was 5 (Dekker, 1983;Gomes et al, 1992;Royer and Nakkas, 1991). The optimum pH was also around 5 in similar experiments on fungal xylanases (Milagres et al, 1993;Gandhi et al, 1994;Sigoillot et al, 2002).…”

“…Xylan is a potential significant resource for renewable biomass, which can be utilized as a substrate for the preparation of many products such as fuels, solvents and pharmaceuticals. Corn, wheat stem, barley shells and similar material contain xylan (Gomes et al, 1992;Gawande and Kamat, 1999;Beg et al, 2001;Subramaniyan and Prema, 2002). On the other hand, xylanases (E.C.…”

Xylanase production with xylan rich lignocellulosic wastes by a local soil isolate of Trichoderma viride

“…The ability of various Trichoderma spp. to produce xylanases on various lignocellulosics has been reported (7,19,20,22). This paper presents the potential of a local soil isolate of Trichoderma viride (S 34), to produce xylanase on various feed stuffs like wheat straw, maize straw, bajra straw, oat hay, barseem hay and jowar straw under submerged fermentation conditions (SmF).…”

Production of a high level of cellulase-free xylanase by the thermophilic fungus Thermomyces lanuginosus in laboratory and pilot scales using lignocellulosic materials

“…Aspergillus, Gliocladium, Schizophyllum and Trichoderma, are not thermostable at 50°C or above, the hydrolytic efficiencies of these enzymes are low (Dekker and Richards 1976;Gomes et al 1989Gomes et al , 1992Poutanen 1988;Senior et al 1989;Steiner et al 1987). Moreover, all these fungi produce appreciable amounts of cellulase activity.…”