Production of C20 9S- and C22 11S-hydroxy fatty acids by cells expressing Shewanella hanedai arachidonate 9S-lipoxygenase

Kim, Min-Ju; Liu, Jin; Kim, Sueun; Shin, Kyung‐Chul; Oh, Deok‐Kun

doi:10.1007/s00253-022-12285-3

Cited by 5 publications

(3 citation statements)

References 47 publications

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“…The data for all bacteria included in this statistical analysis are provided in Table S3. The bar at the right shows total binding site bottom volumes of experimentally characterized LOXs [147,[149][150][151][152][153][154] along with their activities. "Human-related" and "plant-related" groups correspond (ω-6)-LOX activity while "marine-related" group is shifted towards (ω-12)-LOX activity.…”

Section: Vertebrate" Samples)mentioning

confidence: 99%

“…Compared with the binding site bottom volumes of experimentally characterized LOXs [147,[149][150][151][152][153][154] (calculated by the same method), the "human-related" and "plant-related" LOX groups appear to correspond to (ω-6)-LOXs. The Pseudomonas aeruginosa and Burkholderia thailandensis LOXs (both belonging to the "human/vertebrate" group in our statistics) have total binding site bottom volumes (472.2×10 -3 nm 3 and 475×10 -3 nm 3 , respectively) near to the mean of the "human-related" group.…”

Section: Vertebrate" Samples)mentioning

confidence: 99%

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Bacterial lipoxygenases are associated with host-microbe interactions and may provide cross-kingdom host jumps

Kurakin

2022

Preprint

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In this bioinformatic research, we studied the association of bacterial lipoxygenases (LOXs) with pathogenic and symbiotic traits by text networks analysis, phylogenetic analysis, and statistical analysis of molecular structure. We found that bacterial lipoxygenases are associated with a broad host range - from coral to plants and humans. In humans, bacterial LOXs are associated with opportunistic and nosocomial infections as well as with affecting specific patient populations like cystic fibrosis patients. Moreover, bacterial LOXs are associated with plant-human (or human-plant) host jumps in emerging pathogens. We also inferred a possible mechanism of such host jumps working via a host's oxylipin signalling "spoofing".

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Section: Vertebrate" Samples)mentioning

confidence: 99%

Section: Vertebrate" Samples)mentioning

confidence: 99%

Bacterial lipoxygenases are associated with host-microbe interactions and may provide cross-kingdom host jumps

Kurakin

2022

Preprint

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“…Nevertheless, the levels of RvE4 and its enantiomer produced by the recombinant cells were found to be low, indicating that the enhanced production via whole-cell biotransformation is needed to facilitate the industrial-scale synthesis of SPMs. Whole-cell biotransformation can be improved by increasing substrate solubility via the addition of solvents and polymers (Kim et al 2023) and by decreasing the toxicity of the substrate and product at high concentrations via the addition of an adsorbent resin (Kim et al 2020). To improve the biotransformation of EPA into RvE4 and its enantiomer, the addition of solvent, polymer, and resin of optimized concentrations and types is required.…”

mentioning

confidence: 99%

Biotransformation of eicosapentaenoic acid into the dihydroxyeicosapentaenoic acids resolvin E4 and its enantiomer by 15S- and 15R-lipoxygenases expressed in Escherichia coli

Lee,

Park,

Shin

et al. 2024

Preprint

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Objectives To enhance the production of resolvin E4 (RvE4) or its enantiomer from eicosapentaenoic acid (EPA), Archangium violaceum 15S-lipoxygenase (15S-LOX) or Sorangium cellulosum 15R-LOX was expressed in Escherichia coli with solvent, polymer, and adsorbent resin, respectively. Results The concentrations of cells and substrate and the types and concentrations of solvent, polymer, and resin were optimized for the biotransformation of EPA into RvE4 (5S,15S-dihydroxyeicosapentaenoic acid) and its enantiomer (5R,15R-dihydroxyeicosapentaenoic acid). Under optimized conditions, A. violaceum 15S-LOX and S. cellulosum 15R-LOX expressed in E. coli converted 6.0 mM (1.8 g L−1) EPA into 4.3 mM (1.4 g L−1) RvE4 and 5.8 mM (1.9 g L−1) RvE4 enantiomer in 60 min, with productivities of 4.3 and 5.8 mM h−1 and molar conversions of 72 and 97%, respectively. The concentrations of RvE4 and its enantiomer resulting from the conversion of EPA with solvent, polymer, and resin were 3.1- and 5.3-fold higher than those without additives, respectively. Conclusions The concentrations, productivities, and conversions of RvE4 and its enantiomer were increased by optimizing the concentrations of cells and substrate and the types and concentrations of solvent, polymer, and adsorbent resin.

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Efficient biotransformation of docosahexaenoic acid-rich oils into the lipid mediator resolvin D5 by cells expressing 15S-lipoxygenase using a bioreactor

Lee,

Park,

Shin

et al. 2023

Bioresource Technology

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Production of C20 9S- and C22 11S-hydroxy fatty acids by cells expressing Shewanella hanedai arachidonate 9S-lipoxygenase

Cited by 5 publications

References 47 publications

Bacterial lipoxygenases are associated with host-microbe interactions and may provide cross-kingdom host jumps

Bacterial lipoxygenases are associated with host-microbe interactions and may provide cross-kingdom host jumps

Biotransformation of eicosapentaenoic acid into the dihydroxyeicosapentaenoic acids resolvin E4 and its enantiomer by 15S- and 15R-lipoxygenases expressed in Escherichia coli

Efficient biotransformation of docosahexaenoic acid-rich oils into the lipid mediator resolvin D5 by cells expressing 15S-lipoxygenase using a bioreactor

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