Production of biomass and recombinant human-like collagen in Escherichia coli processes with different CO2 pulses

Xue, Wenjiao; Fan, Daming; Shang, Longan; Zhu, Chen H.; Xiao, Xu; Yu, Yuan Y.

doi:10.1007/s10529-008-9852-9

Cited by 9 publications

(3 citation statements)

References 18 publications

(21 reference statements)

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“…Expressing a fully functional prolyl hydroxylase in E. coli has also been very challenging [40]. Therefore, most work on E. coli produced collagen involves only small functional sections of human collagen, or is on human-like collagen [41][42][43][44]. Relatively little work on recombinant human collagen production has been performed in E. coli as compared with eukaryotic organisms, suggesting challenges, and hence opportunities for research in this area.…”

Section: Shortcomings Of Extracted Collagenmentioning

confidence: 99%

Production of recombinant collagen: state of the art and challenges

Wang

Lew

Jayaraman

et al. 2017

Engineering Biology

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Collagen, which is often used in healthcare materials and biomedical research, is largely extracted from animal sources. Recombinant human collagen has the potential to be a promising alternative to animal collagen which has many shortcomings, including immunogenicity and lack of biocompatibility. Currently, recombinant human collagen has been expressed in both eukaryotic and prokaryotic hosts with varying degrees of success. One issue with recombinant collagen across all hosts is the inability to achieve full length collagen with native amounts of post-translational modifications, prompting much exciting research in this direction. There has also been much effort in improving yield and biomimicry of recombinant collagen. This review discusses collagen structure and current methods for extracting animal collagen, before introducing current research in synthesising recombinant human collagen in various hosts, and finally highlighting challenges in the field.

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Section: Shortcomings Of Extracted Collagenmentioning

confidence: 99%

Production of recombinant collagen: state of the art and challenges

Wang

Lew

Jayaraman

et al. 2017

Engineering Biology

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“…Se han publicado algunos trabajos en donde se mejora esta tecnología, sobre todo para producir colágeno humano. Entre las estrategias empleadas podemos mencionar el aumento del crecimiento y expresión por medio de variaciones del CO 2 [20] , aumentando la presión para mejorar la transferencia de oxígeno y la optimización de la relación carbono/nitrógeno en el cultivo [21] .…”

Section: Colágenaunclassified

Recombinant Collagens for Tissue Engineering Scaffolds

Paredes-Puerto¹

2017

Rev. Mex. Ing. Biomed.

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Las colágenas son cada día más atractivas en la fabricación de andamios para Ingeniería de Tejidos, por su biocompatibilidad, manejo y capacidad de producirlas industrialmente. El objetivo del presente artículo fue presentar un análisis sobre el avance en la investigación, el desarrollo y producción de colágenas recombinantes de humano, los sistemas de producción y sus usos en Ingeniería de Tejidos. Se realizó una revisión de la literatura científica internacional arbitrada en bases de datos como Scopus, PubMed y Google Académico y se empleó aquella relevante a nuestro objetivo. Se encontró que el desarrollo de colágenas recombinantes de humano muestra un avance significativo y en la actualidad los sistemas de expresión, como bacterias y plantas, presentan ventajas sobre la calidad de la estructura y la biocompatibilidad, aunque con rendimientos todavía bajos. Mientras que existe escasa información sobre sus aplicaciones en Ingeniería de Tejidos, principalmente cartílago y hueso, en modelos animales y estudios clínicos. En las fuentes de información no se incluyeron patentes, por lo que nuestros hallazgos están limitados a publicaciones científicas. El presente trabajo, presenta los avances más recientes sobre la ingeniería de colágenas recombinantes y sus aplicaciones biomédicas en fabricación de tejidos con potencial uso clínico. Por lo que su factibilidad en la medicina regenerativa es prometedor y se requiere mayor investigación que permita su aplicación en un futuro cercano.

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“…More recently, there have been a number of scattered reports on optimization of E. coli for enhanced biomass production of recombinant human-like collagens. These include enhanced growth and expression by controlled CO 2 pulsing (Xue et al 2009), increasing the fermentor pressure and elevating the oxygen transfer rate (Ma et al 2010), medium optimization of the carbon/nitrogen ratio (Guo et al 2010) and assimilation of acetic acid production by glucose starvation (Xue et al 2010). In these limited studies from these Chinese groups, the increase in biomass and product yield has been significant.…”

Section: Expression Of Mammalian Collagenmentioning

confidence: 99%

Recombinant protein scaffolds for tissue engineering

2012

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New biological materials for tissue engineering are now being developed using common genetic engineering capabilities to clone and express a variety of genetic elements that allow cost-effective purification and scaffold fabrication from these recombinant proteins, peptides or from chimeric combinations of these. The field is limitless as long as the gene sequences are known. The utility is dependent on the ease, product yield and adaptability of these protein products to the biomedical field. The development of recombinant proteins as scaffolds, while still an emerging technology with respect to commercial products, is scientifically superior to current use of natural materials or synthetic polymer scaffolds, in terms of designing specific structures with desired degrees of biological complexities and motifs. In the field of tissue engineering, next generation scaffolds will be the key to directing appropriate tissue regeneration. The initial period of biodegradable synthetic scaffolds that provided shape and mechanical integrity, but no biological information, is phasing out. The era of protein scaffolds offers distinct advantages, particularly with the combination of powerful tools of molecular biology. These include, for example, the production of human proteins of uniform quality that are free of infectious agents and the ability to make suitable quantities of proteins that are found in low quantity or are hard to isolate from tissue. For the particular needs of tissue engineering scaffolds, fibrous proteins like collagens, elastin, silks and combinations of these offer further advantages of natural well-defined structural scaffolds as well as endless possibilities of controlling functionality by genetic manipulation.

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Production of biomass and recombinant human-like collagen in Escherichia coli processes with different CO2 pulses

Cited by 9 publications

References 18 publications

Production of recombinant collagen: state of the art and challenges

Production of recombinant collagen: state of the art and challenges

Recombinant Collagens for Tissue Engineering Scaffolds

Recombinant protein scaffolds for tissue engineering

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