Production of Biofuels (H2&CH4) from Food Leftovers via Dual-Stage Anaerobic Digestion: Enhancement of Bioenergy Production and Determination of Metabolic Fingerprinting of Microbial Communities

Abstract

Proper management of food waste has become a major cause of concern over the past few years in both developed and developing countries. This work aimed to properly treat food leftovers through 2-stage anaerobic fermentation at mesophilic temperature for biofuel production (H2 and CH4) and correlate this with the metabolic fingerprints of the most dominant bacterial strains in the two biofermenters. The diversity of bacterial communities in the surface, middle and bottom levels of the hydrogen fermenter as well as in the methane fermenter was examined. Moreover, the phenotypic identification and metabolic fingerprints for the bacterial strains was carried out using Biolog GEN III. The dominant bacterial strain responsible for hydrogen production was Bacillus amyloliquefaciens. While, Stenotrophomonas rhizophila was the most dominant in the methane fermenter. The total energy production was improved by 22.2% in case of increasing HRT for the first fermenter from 17h to 34h.

Maximizing Bio-Hydrogen and Energy Yields Obtained in a Self-Fermented Anaerobic Bioreactor by Screening of Different Sewage Sludge Pretreatment Methods

“…Anaerobic digestion (AD) is a common method for the co-digestion of sewage sludge with different substrates [5][6][7]. This process usually produces biogas that forms a mixture of methane (CH 4 ), carbon dioxide (CO 2 ), a small percent of hydrogen gas, hydrogen sulfide, and stabilized digestate [8].…”

A Comprehensive Approach to Azo Dichlorotriazine Dye Treatment: Assessing the Impact of Physical, Chemical, and Biological Treatment Methods through Statistical Analysis of Experimental Data

“…The biological treatment of wastewater has some advantages such as being environmentally friendly, producing less sludge, and being inexpensive, so it is often the most cost-effective alternative compared to other treatment solutions [14,15]. However, the biological treatment systems are not efficacious in the treatment of industrial effluents when utilized independently because these effluents, especially textile effluent, usually contain toxic and/or refractory organic chemicals that are complicated to treat so, the biological treatment should be combined or using dual of biological stage [16]. Consequently, the incorporation of chemical, photochemical, and biological treatment processes has been suggested for these instances.…”

“…Fenton is an efficient advanced treatment process. The hydroxyl radical (OH • ) is formed from the reaction between hydrated Fe +2 and hydrogen peroxide (H 2 O 2 ), which degrade the refractory and toxic organic contaminates present in wastewater [16]. There are many advantages of the Fenton process, such as high performance, inexpensive reagents, H 2 O 2 can decompose into environmentally harmless sorts such as H 2 O and O 2 , and it is conducted at ambient temperature and atmospheric pressure for the organic oxidation [20].…”

An application of advanced oxidation process on industrial crude oily wastewater treatment

“…Recent increases in human activity at the level of home sectors 1 , agricultural sectors 2 and municipal sectors 3 resulting increase in the organic contaminants in wastewater treatment facilities, which causes a discharge to water bodies that does not meet safety and environmental criteria. Due of its hazard, wastewater tainted with oil can actually harm the environment.…”