Sugar beet bagasse was pyrolyzed in a fixed-bed reactor. The temperature of pyrolysis, heating rate, and sweep gas flow rate were varied in the ranges of 350-550 ı C, 10-30 ı C min 1 , and 100-200 cm 3 min 1 , respectively. The maximum oil yield of 26.71% was obtained at a pyrolysis temperature of 500 ı C, heating rate of 30 ı C min 1 , and sweeping gas flow rate of 100 cm 3 min 1 . The elemental analyses and calorific values of the bio-oil were determined, and then the chemical compositions of the bio-oil were investigated using chromatographic and spectroscopic techniques, such as column chromatography and Fourier transform infrared.