Production of bioethanol from corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus

Nikolic, Slobodan; Mojović, Ljiljana; Pejin, Dušanka J.; Rakin, Marica; Vukašinović, Maja

doi:10.1016/j.biombioe.2010.04.008

Cited by 69 publications

(42 citation statements)

References 24 publications

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“…The ethanol productivity of all grafted CMC was higher than ungrafted CMC. Similar observation was also found in the previous report with S. cerevisiae [27,29].…”

Section: Effect Of Initial Glucose Concentration On Ethanol Productionsupporting

confidence: 90%

“…They reported that ethanol production was 8.9% (w/w) using 176 g/L initial glucose concentration by Ca-alginate immobilized S. cerevisiae. Ethanol productivity was determined in the range of 1.19-2.26 g/L h while fermentation time was in the range of 26-74 h [27]. Zhao and Xia immobilized recombinant S. cerevisiae on Ca-alginate beads for production of ethanol.…”

Section: Effect Of Initial Glucose Concentration On Ethanol Productionmentioning

confidence: 99%

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Immobilization of Saccharomyces cerevisiae on to modified carboxymethylcellulose for production of ethanol

Gökgöz

Yi̇ği̇toğlu

2011

Bioprocess Biosyst Eng

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In this work, modified carboxymethylcellulose (CMC) was used as a new support material for production of ethanol. Crosslinked graft copolymers of CMC with N-vinyl-2-pyrrolidone (N-VP) were prepared in different grafting yields. The beads material was characterized by means of fourier transform infrared spectroscopy (FT-IR), differential scanning calorimetry (DSC), scanning electron microscope (SEM) and swelling experiment. Saccharomyces cerevisiae was immobilized using entrapment method in the graft copolymers of carboxymethylcellulose-g-poly(N-vinyl-2-pyrrolidone) (CMC-g-PVP) for ethanol fermentation. The effects of grafting yield, initial glucose concentration and crosslinker concentration on the yield of ethanol process were investigated. Reusability of the immobilized yeasts was investigated and found that the materials can be used four times without losing their activity. Ethanol production increased to 59.3 g/L from 46.4 g/L when percentage of N-VP in the graft copolymer was increased. The highest ethanol productivity was found to be 1.75-2.25 g/L h. Fermentation time decreased with the decreasing of crosslinker concentration. The results suggest that the proposed method for immobilization of Saccharomyces cerevisiae has potential in industrial applications for ethanol process.

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“…The ethanol productivity of all grafted CMC was higher than ungrafted CMC. Similar observation was also found in the previous report with S. cerevisiae [27,29].…”

Section: Effect Of Initial Glucose Concentration On Ethanol Productionsupporting

confidence: 90%

Section: Effect Of Initial Glucose Concentration On Ethanol Productionmentioning

confidence: 99%

Immobilization of Saccharomyces cerevisiae on to modified carboxymethylcellulose for production of ethanol

Gökgöz

Yi̇ği̇toğlu

2011

Bioprocess Biosyst Eng

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“…Selection of a higher ethanol-tolerant yeast strain may prolong the fermentation cycle, which has the potential to increase bioethanol yield. To identify an optimal yeast strain, the viability of various yeast strains under diVerent fermentation conditions can be explored [3,10,18]. Therefore, developing a simple counting method for measuring yeast concentration and viability could be essential for optimizing bioethanol production.…”

Section: Introductionmentioning

confidence: 99%

Direct concentration and viability measurement of yeast in corn mash using a novel imaging cytometry method

Chan¹,

Lyettefi²,

Pirani³

et al. 2010

J Ind Microbiol Biotechnol

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Worldwide awareness of fossil-fuel depletion and global warming has been increasing over the last 30 years. Numerous countries, including the USA and Brazil, have introduced large-scale industrial fermentation facilities for bioethanol, biobutanol, or biodiesel production. Most of these biofuel facilities perform fermentation using standard baker's yeasts that ferment sugar present in corn mash, sugar cane, or other glucose media. In research and development in the biofuel industry, selection of yeast strains (for higher ethanol tolerance) and fermentation conditions (yeast concentration, temperature, pH, nutrients, etc.) can be studied to optimize fermentation performance. Yeast viability measurement is needed to identify higher ethanol-tolerant yeast strains, which may prolong the fermentation cycle and increase biofuel output. In addition, yeast concentration may be optimized to improve fermentation performance. Therefore, it is important to develop a simple method for concentration and viability measurement of fermenting yeast. In this work, we demonstrate an imaging cytometry method for concentration and viability measurements of yeast in corn mash directly from operating fermenters. It employs an automated cell counter, a dilution buffer, and staining solution from Nexcelom Bioscience to perform enumeration. The proposed method enables specific fluorescence detection of viable and nonviable yeasts, which can generate precise results for concentration and viability of yeast in corn mash. This method can provide an essential tool for research and development in the biofuel industry and may be incorporated into manufacturing to monitor yeast concentration and viability efficiently during the fermentation process.

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“…Bioethanol has low production costs, easy of operation and can be obtained from different raw materials materials (Cardona & Sánchez, 2007;Balat et al, 2008;Sage et al, 2009;Nikolic et al, 2010). Any sugar or starch raw material can be converted to ethanol by microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Modeling and kinetic study of bio-ethanol production from soy protein concentrate by-product

et al. 2016

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The aim of this work was to evaluate a non-agitated process of bioethanol production from soybean molasses and the kinetic parameters of fermentation using a strain of Saccharomyces cerevisiae (ATCC  2345). Kinetic experiment was conducted in medium with 30% (w v -1 ) of soluble solids without supplementation or pH adjustment. The maximum ethanol concentration was in 44 hours, the ethanol productivity was 0.946 g L -1 h -1, the yield over total initial sugars (Y 1 ) was 47.87%, over consumed sugars (Y 2 ) was 88.08% and specific cells production rate was 0.006 h -1 . The mathematical polynomial was adjusted to the experimental data and provided very similar parameters of yield and productivity. Based in this study, for one ton of soybean molasses can be produced 103 kg of anhydrous bioethanol.Keywords: alcoholic fermentation; bioethanol; soybean molasses; co-product valorization.Practical Application: Static fermentation showed high yield to ethanol production from soybean molasses.

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Production of bioethanol from corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus

Cited by 69 publications

References 24 publications

Immobilization of Saccharomyces cerevisiae on to modified carboxymethylcellulose for production of ethanol

Immobilization of Saccharomyces cerevisiae on to modified carboxymethylcellulose for production of ethanol

Direct concentration and viability measurement of yeast in corn mash using a novel imaging cytometry method

Modeling and kinetic study of bio-ethanol production from soy protein concentrate by-product

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