Production of biodegradable copolyesters of 3-hydroxybutyrate and 4-hydroxybutyrate by Alcaligenes eutrophus

Kunioka, Masao; Kawaguchi, Yasushi; Doi, Yoshiharu

doi:10.1007/bf00255361

Cited by 176 publications

(94 citation statements)

References 14 publications

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“…Synthesis of copolyesters containing 4HB was found in R. eutropha (11,12,36,38), Hydrogenophaga pseudoflava (5), in recombinant strains of E. coli (9), and in several other bacteria. The high amounts of the copolyester (up to 68.4% by weight) in E. coli JM109(pBPP1) made this strain competitive to other producers.…”

Section: Discussionmentioning

confidence: 99%

A Novel Genetically Engineered Pathway for Synthesis of Poly(Hydroxyalkanoic Acids) in Escherichia coli

Liu

Steinbüchel

2000

Appl Environ Microbiol

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A new pathway to synthesize poly(hydroxyalkanoic acids) (PHA) was constructed by simultaneously expressing butyrate kinase (Buk) and phosphotransbutyrylase (Ptb) genes of Clostridium acetobutylicum and the two PHA synthase genes (phaE and phaC) of Thiocapsa pfennigii in Escherichia coli. The four genes were cloned into the BamHI and EcoRI sites of pBR322, and the resulting hybrid plasmid, pBPP1, conferred activities of all three enzymes to E. coli JM109. Cells of this recombinant strain accumulated PHAs when hydroxyfatty acids were provided as carbon sources. Homopolyesters of 3-hydroxybutyrate (3HB), 4-hydroxybutyrate (4HB), or 4-hydroxyvalerate (4HV) were obtained from each of the corresponding hydroxyfatty acids. Various copolyesters of those hydroxyfatty acids were also obtained when two of these hydroxyfatty acids were fed at equal amounts: cells fed with 3HB and 4HB accumulated a copolyester consisting of 88 mol% 3HB and 12 mol% 4HB and contributing to 68.7% of the cell dry weight. Cells fed with 3HB and 4HV accumulated a copolyester consisting of 94 mol% 3HB and 6 mol% 4HV and contributing to 64.0% of the cell dry weight. Cells fed with 3HB, 4HB, and 4HV accumulated a terpolyester consisting of 85 mol% 3HB, 13 mol% 4HB, and 2 mol% 4HV and contributing to 68.4% of the cell dry weight.

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Section: Discussionmentioning

confidence: 99%

A Novel Genetically Engineered Pathway for Synthesis of Poly(Hydroxyalkanoic Acids) in Escherichia coli

Liu

Steinbüchel

2000

Appl Environ Microbiol

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“…During metabolism of GBL, the lactone ring is opened to yield 4-hydroxybutyric acid. Choi et al (1999) working with Hydrogenophaga pseudoflava [18] and Kunioka et al (1989) working with Cupriavidus necator [28] suggest that ring opening probably occurs within the cell. A pathway for P(3HB-co-4HB) synthesis proposed by , in Cupriavidus necator through the action of a PHA synthase.…”

Section: Effect Of the Glucose And Gbl Concentrations On The Copolymementioning

confidence: 99%

Production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) by Burkholderia sacchari using wheat straw hydrolysates and gamma-butyrolactone

Cesário

Raposo

Almeida

et al. 2014

International Journal of Biological Macromolecules

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Burkholderia sacchari DSM 17165 is able to grow and produce poly(3-hydroxybutyrate) both on hexoses and pentoses. In a previous study, wheat straw lignocellulosic hydrolysates (WSH) containing high C6 and C5 sugar concentrations were shown to be excellent carbon sources for P(3HB) production.Using a similar feeding strategy developed for P(3HB) production based on WSH, fedbatch cultures were developed aiming at the production of the copolymer P(3HB-co-4HB) (poly(3-hydroxybutyrate-co-4-hydroxybutyrate)) by B. sacchari. The ability of this * Corresponding author. Tel.: +351 21 8419137; Fax: +351 21 8419062.e-mail address: teresa.cesario@tecnico.ulisboa.pt 2 strain to synthesize P(3HB-co-4HB) was first shown in shake flasks using gammabutyrolactone (GBL) as precursor of the 4HB units.Fed-batch cultures using glucose as carbon source (control) and GBL were developed to achieve high copolymer productivities and 4HB incorporations. The attained P(3HB-co-4HB) productivity and 4HB molar % were 0.7 g/(L·h) and 4.7 molar %, respectively.The 4HB incorporation was improved to 6.3 and 11.8 molar % by addition of 2 g/L propionic and acetic acid, respectively. When WSH were used as carbon source under the same feeding conditions, the values achieved were 0.5 g/(L·h) and 5.0 molar %, respectively. Burkholderia sacchari, a strain able to produce biopolymers based on xylose-rich lignocellulosic hydrolysates, is for the first time reported to produce P(3HB-co-4HB) using gamma butyrolactone as precursor.

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“…P(3HB-co-4HB) was produced by R. eutropha in nitrogen-limited cultures containing 4-hydroxybutyric acid [1], g-butyrolactone [2] or 1,4-butanediol [3] as the carbon source, whose composition was varied from 0 to 34 mol% 4HB, depending on the composition of carbon substrates supplied in feed. Recently, random copolymers P(3HB-co-4HB) with a wide range of compositions varying from 0 to 83 mol% 4HB were produced by A. latus from the mixed carbon substrates of 3-hydroxybutyric and 4-hydroxybutyric acid [4], though these were expensive.…”

Section: Introductionmentioning

confidence: 99%

Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) produced by Comamonas acidovorans

Mitomo

Hsieh

Nishiwaki

et al. 2001

Polymer

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Poly(3-hydoxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] was biosynthesized by Comamonas acidovorans with mixed carbon sources of n-butyric acid and 1,4-butanediol. P(3HB) and P(3HB-co-94 mol% 4HB) were obtained by fermentation with sole carbon source of n-butyric acid and 1,4-butanediol, respectively. The content of 4HB component in P(3HB-co-4HB) increased as the composition of 1,4-butanediol in mixed carbon sources increased. These biosynthesized copolymers are not homogeneous, but mixtures of copolymers having different 4HB contents. Fractionation was carried out to obtain fractionated copolymers having a narrower distribution of composition. The change in structural and physical properties of the fractionated copolymers were investigated against the 4HB contents. Crystallinities of P(3HB-co-4HB)s were estimated using density values and the heat of fusion of P(3HB) measured was 125 J g 21 and that of P(4HB) was 76 J g 21 . q

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Production of biodegradable copolyesters of 3-hydroxybutyrate and 4-hydroxybutyrate by Alcaligenes eutrophus

Cited by 176 publications

References 14 publications

A Novel Genetically Engineered Pathway for Synthesis of Poly(Hydroxyalkanoic Acids) in Escherichia coli

A Novel Genetically Engineered Pathway for Synthesis of Poly(Hydroxyalkanoic Acids) in Escherichia coli

Production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) by Burkholderia sacchari using wheat straw hydrolysates and gamma-butyrolactone

Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) produced by Comamonas acidovorans

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