Production of bioactive triterpenes by Eriobotrya japonica calli

Taniguchi, Shoko; Imayoshi, Yoko; Kobayashi, Eri; Takamatsu, Yoshie; Ito, Hideyuki; Hatano, Tsutomu; Sakagami, Hiroshi; Tokuda, Harukuni; Nishino, Hoyoku; Sugita, Daigo; Shimura, Susumu; Yoshida, Takashi

doi:10.1016/s0031-9422(01)00455-1

Cited by 259 publications

(144 citation statements)

References 24 publications

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“…Alternative studies and our previous and present studies indicate that EGCG inhibits EBV lytic infection, though the mechanism is not well understood (26,(57)(58)(59). In our previous study, CNE1-LMP1 and B95.8 cells with EBV spontaneous lytic replication were used to mimic the natural state of infected cells, and it was indicated that EGCG inhibited EBV spontaneous lytic replication by inhibiting activation of MEK/ERK1/2 and PI3-K/Akt signaling (26).…”

Section: Discussionmentioning

confidence: 69%

(-)-Epigallocatechin‑3‑gallate inhibition of Epstein‑Barr virus spontaneous lytic infection involves downregulation of latent membrane protein 1

Liu

Tang

et al. 2017

Exp Ther Med

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Abstract. The Epstein-Barr virus (EBV) lytic cycle contributes to the development of EBV-associated diseases. EBV-encoded latent membrane protein 1 (LMP1) is key to EBV lytic replication, and our previous work indicated that epigallocatechin-3-gallate (EGCG) inhibited constitutive EBV lytic infection through the suppression of LMP1-activated phosphoinositide 3-kinase/Akt and mitogen-activated protein kinase kinase/extracellular signal-related protein kinase 1/2 signaling. The present study demonstrated that LMP1 in CNE-LMP1 constructed cells significantly induced the expression of the EBV lytic proteins BZLF1 (P<0.001) and BMRF1 (P<0.05) compared with CNE1 cells. Following treatment with a specific DNAzyme that targets LMP1, significantly reduced protein expression levels of BZLF1 and BMRF1 in EBV-associated epithelial carcinoma CNE1-LMP1 cells (P<0.001 and P<0.01, respectively) and lymphoma B95.8 cells (both P<0.01) were observed. Furthermore, EGCG significantly inhibited the mRNA and protein expression levels of LMP1 (P<0.05) in an apparent dose-dependent manner in CNE1-LMP1 and B95.8 cells. Thus, the present findings indicated that the molecular mechanism underlying EGCG inhibition of EBV lytic infection involves downregulation of LMP1. IntroductionEpstein-Barr virus (EBV) is a human herpes virus that infects over 90% of the human population worldwide (1). EBV is suggested to be an environmental factor associated with the development of several human malignancies, including nasopharyngeal carcinoma (NPC), Burkitt's lymphoma, Hodgkin's disease (HD), gastric cancer, natural killer/T-cell lymphoma, acquired immune deficiency syndrome-and transplantation-associated lymphoma (2,3), breast carcinoma, and hepatocellular carcinoma (4,5). EBV, as all other herpes viruses, may establish a latent or lytic infection in host cells (6). Notably, studies suggest that EBV reactivation into a lytic cycle may contribute to the pathogenesis of malignancies (7,8). EBV lytic infection in vivo has been identified by elevated antibody titers against EBV lytic antigens and by increased viral DNA load in the serum/plasma, and these observations correspond with advanced cancer stages, poor prognosis or tumor recurrence following therapy (9,10). Additionally, serological studies have indicated that EBV lytic infection may occur months or years prior to a clinical diagnosis of NPC, HD or Burkitt's lymphoma, which suggests EBV lytic infection may be a risk factor for cancer development (11-13).Green tea contains (-)-epigallocatechin-3-gallate (EGCG), which is reported to have antioxidant, antibacterial and antitumor effects (14,15). EGCG has been indicated to modulate multiple signaling pathways, including the phosphoinositide 3-kinase (PI3-K)/Akt and mitogen-activated protein kinase (MAPK) signaling pathways, which may enable it to exert its cancer chemopreventive and therapeutic effects (16,17).EBV encoded latent membrane protein 1 (LMP1), which is considered to have oncogenic properties, has been identified in 90% of patients with NPC...

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Section: Discussionmentioning

confidence: 69%

(-)-Epigallocatechin‑3‑gallate inhibition of Epstein‑Barr virus spontaneous lytic infection involves downregulation of latent membrane protein 1

Liu

Tang

et al. 2017

Exp Ther Med

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“…Although its activity was relatively weak in several studies (Njoku et al, 1997;Kim et al, 2000;Chiang et al, 2003b;Fu et al, 2005), it did show some selectivity (Taniguchi et al, 2002). In vitro studies into 83's effects indicated that it acted by inducing apoptosis (Fernandes et al, 2003;Huang et al, 2003b;Urech et al, 2005), but it also had other effects: it inhibited the invasive, adhesive, and migration abilities of lung cancer cells (Huang et al, 2003b); showed antiangiogenic activity, possibly by inhibiting proliferation of vascular endothelial cells (Sohn et al, 1995); and induced differentiation, which does not proceed normally in some cancer types (Umehara et al, 1992).…”

Section: Cancermentioning

confidence: 95%

“…Oleanolic acid (83), 3-acetoxy-oleanolic acid (84), and katononic acid (90) all inhibited TPA-induced EBV-EA (Ohigashi et al, 1986;Konoshima et al, 1987;Taniguchi et al, 2002;Ismail et al, 2003;Banno et al, 2004); 84 was more active than 83, and 90 was less active. Oleanolic acid (83) was a good inhibitor of the mutagenicity of benzo[a]pyrene in a bacterial assay (Niikawa et al, 1993).…”

Section: Cancermentioning

confidence: 99%

“…3β-Acetoxy-ursolic acid (103) was also antimutagenic in the umu test (Miyazawa et al, 2005) and had antitumor activity in vivo (Dominic and Subbaiyan, 1993) and some aromatase-inhibiting activity in vitro (Jeong et al, 2000). Although 2α-hydroxy-ursolic acid (104) inhibited TPA-induced EBV-EA (Banno et al, 2004) and showed good cytotoxicity to several cancer cell lines, seeming to be particularly effective against solid tumors Numata et al, 1989;Ahn et al, 1998;El-Hossary et al, 2000;Kim et al, 2000), in one study it was as cytotoxic to normal human fibroblasts as to two tumor cell lines (Taniguchi et al, 2002). The cytotoxicity seems to be related to the compound's ability to inhibit protein kinase C (EC 2.7.11.13) (Ahn et al, 1998).…”

Section: Insecticidal Activitymentioning

confidence: 99%

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Medicinal chemistry and pharmacology of genus Tripterygium (Celastraceae)

et al. 2007

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Plants in the genus Tripterygium, such as Tripterygium wilfordii Hook. f., have a long history of use in traditional Chinese medicine. In recent years there has been considerable interest in the use of Tripterygium extracts and of the main bioactive constituent, the diterpene triepoxide triptolide (1), to treat a variety of autoimmune and inflammation-related conditions. The main mode of action of the Tripterygium extracts and triptolide (1) is the inhibition of expression of proinflammatory genes such as those for interleukin-2 (IL-2), inducible nitric oxide synthase (iNOS), tumor necrosis factor-α (TNF-α), cyclooxygenase-2 (COX-2) and interferon-gamma (IFN-γ). The efficacy and safety of certain types of Tripterygium extracts were confirmed in human clinical trials in the US and abroad. Over 300 compounds have been identified in the genus Tripterygium, and many of these have been evaluated for biological activity. The overall activity of the extract is based on the interaction between its components. Therefore, the safety and efficacy of the extract cannot be fully mimicked by any individual constituent. This review discusses the biochemical composition and biological and pharmacological activities of Tripterygium extracts, and their main bioactive components.

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“…Ensaios farmacológicos têm sido conduzidos, a fim de pesquisar possíveis agentes antitumorais (ITO et al, 2000, antioxidantes , hipoglicemiantes (TOMMASI et al, 1991) e inibidores virais (TOMMASI et al, 1992b;TANIGUCHI et al, 2002) O presente trabalho objetivou estudar a morfo-anatomia das folhas de E. japonica, a fim de fornecer subsídios farmacognósticos ao controle de qualidade da droga vegetal e informações taxonômicas à família.…”

Section: Introductionunclassified

Morfo-anatomia das folhas da nespereira Eriobotrya japonica Lindl., Rosaceae

Souza¹,

Santos²,

Duarte³

et al. 2003

Rev. bras. farmacogn.

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RESUMO: Eriobotrya japonica, Rosaceae, é uma espécie conhecida popularmente como nespereira, cujas folhas são empregadas na medicina tradicional como agente hipoglicemiante e no tratamento de doenças da pele. O presente trabalho objetivou caracterizar morfo-anatomicamente as folhas, a fim de fornecer subsídios farmacognósticos ao controle de qualidade da droga vegetal e taxonômicos à família. O material botânico foi fixado e submetido às técnicas usuais de microscopia ótica e eletrônica de varredura. As folhas são simples, alternas, lanceoladas, de limbo inteiro e margem denteada. A epiderme foliar é uniestratificada e revestida por cutícula espessada e levemente estriada. Tricomas tectores unicelulares longos são encontrados em ambas as faces, sendo numerosos na abaxial. A folha é hipoestomática, revelando estômatos anomocíticos. O mesofilo é dorsiventral e os feixes vasculares de pequeno porte são colaterais e podem apresentar extensão de bainha. A nervura principal possui formato biconvexo e o pecíolo circular, sendo ambos percorridos por feixes vasculares anficrivais. Foram observados idioblastos contendo cristais de oxalato de cálcio, mucilagem e compostos fenólicos. UNITERMOS: Eriobotrya japonica; Rosaceae; nespereira; ameixeira-japonesa; morfo-anatomia.ABSTRACT: Leaf morpho-anatomy of loquat Eriobotrya japonica Lindl., Rosaceae. The leaves of Eriobotrya japonica, Rosaceae, popularly known as loquat, are used in folk medicine for their hypoglycemic properties and to treat cutaneous diseases. The aim of this work was to study the leaf morpho-anatomy of the drug for pharmacognostic and taxonomic purposes. The botanical material was prepared for the usual optical and scanning microtechniques. The leaves are simple, alternate and lanceolate, with entire blade and dentate margins. The epidermis is uniseriate and coated by a thick and slightly striated cuticle. Both surfaces are hairy, particularly the lower, with long unicellular non-glandular trichomes. Anomocytic stomata occur exclusively on the lower surface. The mesophyll is dorsiventral and the small bundles are collateral and may present sheath extensions. The midrib has got biconvex shape and the petiole round contour, both showing amphicrival bundles. Idioblasts containing calcium oxalate crystals, mucilage and phenolic compounds were observed.

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Production of bioactive triterpenes by Eriobotrya japonica calli

Cited by 259 publications

References 24 publications

(-)-Epigallocatechin‑3‑gallate inhibition of Epstein‑Barr virus spontaneous lytic infection involves downregulation of latent membrane protein 1

(-)-Epigallocatechin‑3‑gallate inhibition of Epstein‑Barr virus spontaneous lytic infection involves downregulation of latent membrane protein 1

Medicinal chemistry and pharmacology of genus Tripterygium (Celastraceae)

Morfo-anatomia das folhas da nespereira Eriobotrya japonica Lindl., Rosaceae

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