Production of Bioactive Substances to Alleviates Hangover and Ethanol-Induced Liver Damage through Fermentation of Oenanthe javanica Using Lactiplantibacillus plantarum

Abstract

The purpose of this study is to evaluate the effect of the bioconversion products of Oenanthe javanica extract fermented by Lactiplantibacillus plantarum (OEFL) on relieving hangovers and improving liver function. In addition, the bioactive substance of the OEFL, which alleviates hangover and ethanol-induced liver damage, was identified and its bioactive property was verified through in vivo experiments. In major substances analysis using high-performance liquid chromatography, OEFL produced 9.5-fold higher p-coumaric acid than the O. Javanica extract (OE). In addition, considering that quinic acid, which is not present in the OE, was produced in the OEFL it was confirmed that chlorogenic acid was decomposed into quinic acid by bioconversion. In the in vivo experiment using Sprague-Dawley rats, the OEFL and p-coumaric acid diets reduced blood ethanol, acetaldehyde, GPT, and ALP concentrations, increasing blood albumin concentrations compared to ethanol-administered groups, demonstrating that OEFL and p-coumaric acid, the main substance in the OEFL, improved ethanol-induced liver damage. Furthermore, the OEFL and its main bioactive substance, p-coumaric acid, alleviated liver fibrosis by downregulating TGF-β, SMAD-2, SMAD-4, α-SMA, and upregulating MMP-1. Therefore, OEFL is expected to be used as a functional food or pharmaceutical material as it has been confirmed to effectively relieve hangovers, prevent liver damage, and delay liver fibrosis in ethanol-induced liver damages.

Lactobacillus plantarum J26 Alleviating Alcohol-Induced Liver Inflammation by Maintaining the Intestinal Barrier and Regulating MAPK Signaling Pathways

“…At the same time, pre-intake of L. plantarum J26 also improved alcohol-induced liver pathological changes, and the morphological structure of liver tissue in the HLP group was the most similar to that in the NC group. Kirpich et al [ 21 ] reduced ALT and AST activity in serum of patients with ALD by administering Bifidobacterium bifidum and Lactobacillus plantarum 8PA3 Da et al [ 22 ] used lactic acid bacteria to ferment cress, and the products obtained could also inhibit the increase of ALT, AST, and ALP activities in the serum of alcohol-exposed mice, and ameliorated alcohol-induced histopathological alterations in the liver. Based on the enzymatic evaluation of liver function, anatomical and pathological observations, it was tentatively concluded that L. plantarum J26 had a significant improvement effect on alcoholic liver injury.…”

Enhancement of bioactive compounds through bioconversion of Oenanthe javanica using Lactiplantibacillus plantarum

“…According to a previous study by Gam et al (2022), chlorogenic acid was bioconverted into caffeic acid as evaluated via HPLC, and they intended to confirm it using HPLC-MS/MS. We compared the distribution of molecular weights by HPLC-MS/MS to determine the main bioactive compounds in OJE and BOE.…”